Pediatric Nephrology
Pediatr Nephrol (1991) 5:582-586 9 IPNA 1991
Original article Mieroheterogeneity of urinary albumin and tubular proteinuria in juvenile diabetes mellitus Martin Riesl, Karl Schiirerl, Reinhard Wartha 1, Hildgund Schmidt 1, and Dieter Gekle2 Divisions of PediatricNephrologyand MetabolicDisorders, 1UniversityChildren'sHospital,Im NeuenheimerFeld 150, W-6900 Heidelberg, FederalRepublicof Germany,2 UniversityChildren'sHospital,WNzburg, FederalRepublic of Germany ReceivedJanuary3, 1990;receivedin revisedform August 23, December 18, 1990; acceptedJanuary29, 1991
Abstract. We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, ~2-microglobulin and molecular size distribution of urinary proteins were measured, the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein (P
Pediatr Nephrol (1991) 5:582-586 9 IPNA 1991
Original article Mieroheterogeneity of urinary albumin and tubular proteinuria in juvenile diabetes mellitus Martin Riesl, Karl Schiirerl, Reinhard Wartha 1, Hildgund Schmidt 1, and Dieter Gekle2 Divisions of PediatricNephrologyand MetabolicDisorders, 1UniversityChildren'sHospital,Im NeuenheimerFeld 150, W-6900 Heidelberg, FederalRepublicof Germany,2 UniversityChildren'sHospital,WNzburg, FederalRepublic of Germany ReceivedJanuary3, 1990;receivedin revisedform August 23, December 18, 1990; acceptedJanuary29, 1991
Abstract. We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, ~2-microglobulin and molecular size distribution of urinary proteins were measured, the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein (P
