Mutagenesis vol. 29 no. 6 pp. 427–431 Advance Access publication 16 September 2014
doi:10.1093/mutage/geu044
Micronuclei and nuclear abnormalities in buccal mucosa cells in patients with anorexia and bulimia nervosa
This is the first report in women with BN, which should be studied in the future.
Grupo Internacional Facultad de Medicina, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan, Jalisco 45129, México, 2Maestría en Nutrición Clínica, Universidad del Valle de Atemajac, Av. Tepeyac 4800, Fraccionamiento Prados Tepeyac, Zapopan, Jalisco 45050, México, 3Dirección de Educación e Investigación, UMAE Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Belisario Dominguez 1000, Colonia Independencia, Guadalajara, Jalisco 44340, México, 4Departamento de Salud Pública, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez 2100, Nextipac, Zapopan, Jalisco 54110, México, 5Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Hospital 308, El Retiro, Guadalajara, Jalisco 44280, México and 6División de Neurociencias, Centro de Investigación Biomédica de Occidente, IMSS, Sierra Mojada 800, Colonia Independencia, Guadalajara, Jalisco 44320, México
Introduction
1
*To whom correspondence should be addressed. Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Hospital 308, Colonia El Retiro, CP 44280 Guadalajara, Jalisco, México. Tel/Fax: 52(33)36145568; Email: [email protected] Received on June 4, 2014; revised on July 11, 2014; accepted on August 6, 2014
The aim of this study is to assess the frequency of micronucleated cell (MNC) and nuclear abnormalities (NA) in the buccal mucosa cells of females with anorexia nervosa (AN) or bulimia nervosa (BN), compared with healthy women. Individuals with AN and BN have inadequate feeding and compensatory behaviour to avoid weight gain. These behaviours can cause extreme body stress, thereby inducing DNA damage. In a cross-sectional study, we assessed the frequency of MNC and NA in the buccal mucosa cells of female participants with AN or BN. All of these patients had been admitted to a private clinic for the treatment of eating disorders after diagnosis with AN (n = 10) or BN (n = 7) according to the DSM-IV. Age-matched healthy female participants (n = 17) composed the control group. Oral mucosa samples were collected, fixed, stained by acetoorcein/fast green and microscopically examined. Normal cells, MNC and NAs were counted within a 2000 cell sample. The results were analyzed with the Kruskal–Wallis and Mann–Whitney tests. Differences were observed in the frequency of MNC in healthy females (1.2 ± 0.9) versus that of patients with AN (3.4 ± 1.5) (P
doi:10.1093/mutage/geu044
Micronuclei and nuclear abnormalities in buccal mucosa cells in patients with anorexia and bulimia nervosa
This is the first report in women with BN, which should be studied in the future.
Grupo Internacional Facultad de Medicina, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan, Jalisco 45129, México, 2Maestría en Nutrición Clínica, Universidad del Valle de Atemajac, Av. Tepeyac 4800, Fraccionamiento Prados Tepeyac, Zapopan, Jalisco 45050, México, 3Dirección de Educación e Investigación, UMAE Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Belisario Dominguez 1000, Colonia Independencia, Guadalajara, Jalisco 44340, México, 4Departamento de Salud Pública, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez 2100, Nextipac, Zapopan, Jalisco 54110, México, 5Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Hospital 308, El Retiro, Guadalajara, Jalisco 44280, México and 6División de Neurociencias, Centro de Investigación Biomédica de Occidente, IMSS, Sierra Mojada 800, Colonia Independencia, Guadalajara, Jalisco 44320, México
Introduction
1
*To whom correspondence should be addressed. Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Hospital 308, Colonia El Retiro, CP 44280 Guadalajara, Jalisco, México. Tel/Fax: 52(33)36145568; Email: [email protected] Received on June 4, 2014; revised on July 11, 2014; accepted on August 6, 2014
The aim of this study is to assess the frequency of micronucleated cell (MNC) and nuclear abnormalities (NA) in the buccal mucosa cells of females with anorexia nervosa (AN) or bulimia nervosa (BN), compared with healthy women. Individuals with AN and BN have inadequate feeding and compensatory behaviour to avoid weight gain. These behaviours can cause extreme body stress, thereby inducing DNA damage. In a cross-sectional study, we assessed the frequency of MNC and NA in the buccal mucosa cells of female participants with AN or BN. All of these patients had been admitted to a private clinic for the treatment of eating disorders after diagnosis with AN (n = 10) or BN (n = 7) according to the DSM-IV. Age-matched healthy female participants (n = 17) composed the control group. Oral mucosa samples were collected, fixed, stained by acetoorcein/fast green and microscopically examined. Normal cells, MNC and NAs were counted within a 2000 cell sample. The results were analyzed with the Kruskal–Wallis and Mann–Whitney tests. Differences were observed in the frequency of MNC in healthy females (1.2 ± 0.9) versus that of patients with AN (3.4 ± 1.5) (P
