ONCOLOGY LETTERS 13: 1386-1392, 2017
1386
MicroRNA-146a affects the chemotherapeutic sensitivity and prognosis of advanced gastric cancer through the regulation of LIN52 ZHIFEN LUO1*, XIQING LI1*, ZUNLAN ZHAO1, XINGLONG YANG2, SHENGJUN XIAO3 and YUN ZHOU1 1
Department of Oncology, Henan Provincial People's Hospital, Zhengzhou, Henan 450003; 2Cancer Institute, Southern Medical University, Guangzhou, Guangdong 510515; 3Department of Pathology, Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China Received July 15, 2015; Accepted November 7, 2016 DOI: 10.3892/ol.2016.5536
Abstract. The present study aimed to evaluate the correlation between the expression of microRNA‑146a (miR‑146a) and its target gene, LIN52, in advanced gastric cancer, and determine their potential effects on chemotherapeutic sensitivity and prognosis. Total RNA was extracted from 93 tissue samples of advanced gastric cancer and corresponding adjacent non‑tumor tissues to quantify the relative expression levels of miR‑146a using reverse transcription‑quantitative polymerase chain reaction analysis. The expression of LIN52 was detected in tumors and normal tissues using immunohistochemical analysis. Correlation analysis was performed to assess the correlation between the expression of miR‑146a and LIN52 and clinicopathological parameters of gastric cancer, including clinical diagnostic specificity, clinical tumor‑necrosis‑metastasis staging, lymph node metastasis, differentiation grade, chemotherapeutic sensitivity and prognosis. The expression of miR‑146a in advanced gastric cancer tissues was lower, compared with that in the adjacent non‑tumor tissues, and was negatively correlated with lymph node metastasis (P
1386
MicroRNA-146a affects the chemotherapeutic sensitivity and prognosis of advanced gastric cancer through the regulation of LIN52 ZHIFEN LUO1*, XIQING LI1*, ZUNLAN ZHAO1, XINGLONG YANG2, SHENGJUN XIAO3 and YUN ZHOU1 1
Department of Oncology, Henan Provincial People's Hospital, Zhengzhou, Henan 450003; 2Cancer Institute, Southern Medical University, Guangzhou, Guangdong 510515; 3Department of Pathology, Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China Received July 15, 2015; Accepted November 7, 2016 DOI: 10.3892/ol.2016.5536
Abstract. The present study aimed to evaluate the correlation between the expression of microRNA‑146a (miR‑146a) and its target gene, LIN52, in advanced gastric cancer, and determine their potential effects on chemotherapeutic sensitivity and prognosis. Total RNA was extracted from 93 tissue samples of advanced gastric cancer and corresponding adjacent non‑tumor tissues to quantify the relative expression levels of miR‑146a using reverse transcription‑quantitative polymerase chain reaction analysis. The expression of LIN52 was detected in tumors and normal tissues using immunohistochemical analysis. Correlation analysis was performed to assess the correlation between the expression of miR‑146a and LIN52 and clinicopathological parameters of gastric cancer, including clinical diagnostic specificity, clinical tumor‑necrosis‑metastasis staging, lymph node metastasis, differentiation grade, chemotherapeutic sensitivity and prognosis. The expression of miR‑146a in advanced gastric cancer tissues was lower, compared with that in the adjacent non‑tumor tissues, and was negatively correlated with lymph node metastasis (P
