Journal of Medical Microbiology Papers in Press. Published January 16, 2015 as doi:10.1099/jmm.0.000024

Journal of Medical Microbiology Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China --Manuscript Draft-Manuscript Number:

JMM-D-14-00040R2

Full Title:

Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China

Short Title:

Identification and susceptibility of vaginal Candida

Article Type:

Standard

Section/Category:

Antimicrobial agents and chemotherapy

Corresponding Author:

Yi-Ming Fan Affiliated Hospital of Guangdong Medical College Zhanjiang, Guangdong CHINA

First Author:

Xiao-Yu Shi

Order of Authors:

Xiao-Yu Shi Yan-Ping Yang Ying Zhang Wen Li Jie-Di Wang Wen-Ming Huang Yi-Ming Fan

Abstract:

　There is limited information regarding molecular epidemiology and antifungal susceptibilities of Candida isolates using Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18-50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and D1/D2 domain. Antifungal susceptibility testing of seven antifungal agents was performed using Neo-Sensitabs tablet diffusion method. Candida species were isolated from 186 cases (61.79%). The most common pathogen was C. albicans (91.4%), followed by C. glabrata (4.3%), C. tropicalis (3.2%), and C. parapsilosis (1.1%). The susceptible rates of C. albicans were higher for caspofungin, voriconazole and fluconazole than those for itraconazole, miconazole ketoconazole and terbinafine (P