INTERNATIONAL JOURNAL OF ONCOLOGY 44: 1041-1055, 2014
Molecular profiling of chordoma STEFANIE SCHEIL-BERTRAM1,2, ROLAND KAPPLER3, ALEXANDRA VON BAER4, ERICH HARTWIG5, MICHAEL SARKAR6, MASSIMO SERRA7, SILKE BRÜDERLEIN1, BETTINA WESTHOFF8, INGO MELZNER1, BIRGIT BASSALY9, JOCHEN HERMS10, HEINZ-HERMANN HUGO11, MICHAEL SCHULTE12 and PETER MÖLLER1 1
Institute of Pathology, University Hospitals of Ulm; 2Institute of Pathology and Cytology, Dr. Horst Schmidt Clinic, Academic Teaching Hospital of University of Mainz, Wiesbaden; 3Department of Pediatric Surgery, Dr. von Hauner Children's Hospital, Ludwig-Maximilian University of Munich, Munich; 4 Department of Orthopedic Trauma, Hand and Reconstructive Surgery, University Hospitals of Ulm; 5 Department of Trauma, Hand and Reconstructive Surgery, Ev. Diakonissenanstalt, Karlsruhe; 6Department of Trauma and Reconstructive Surgery, Karl-Olga-Krankenhaus, Stuttgart, Germany; 7Laboratory of Experimental Oncology, Orthopedic Rizzoli Institute, Bologna, Italy; 8Department of Orthopedics, University of Düsseldorf; 9 Institute of Pathology, University of Giessen; 10Department of Translational Brain Research, DZNE (German Center for Neurodegenerative Diseases) and Ludwig-Maximilian University of Munich, Munich; 11Department of Neurosurgery, University of Kiel, Kiel; 12Department of Trauma and Orthopedic Surgery, Diakoniekrankenhaus, Rotenburg (Wümme), Germany Received October 30, 2013; Accepted December 3, 2013 DOI: 10.3892/ijo.2014.2268 Abstract. The molecular basis of chordoma is still poorly understood, particularly with respect to differentially expressed genes involved in the primary origin of chordoma. In this study, therefore, we compared the transcriptional expression profile of one sacral chordoma recurrence, two chordoma cell lines (U-CH1 and U-CH2) and one chondrosarcoma cell line (U-CS2) with vertebral disc using a high-density oligonucleotide array. The expression of 65 genes whose mRNA levels differed significantly (p2 for 98% of the time. Based on the observations, robust changes can be identified by selecting transcripts with a fold change of >2 for increases and
Molecular profiling of chordoma STEFANIE SCHEIL-BERTRAM1,2, ROLAND KAPPLER3, ALEXANDRA VON BAER4, ERICH HARTWIG5, MICHAEL SARKAR6, MASSIMO SERRA7, SILKE BRÜDERLEIN1, BETTINA WESTHOFF8, INGO MELZNER1, BIRGIT BASSALY9, JOCHEN HERMS10, HEINZ-HERMANN HUGO11, MICHAEL SCHULTE12 and PETER MÖLLER1 1
Institute of Pathology, University Hospitals of Ulm; 2Institute of Pathology and Cytology, Dr. Horst Schmidt Clinic, Academic Teaching Hospital of University of Mainz, Wiesbaden; 3Department of Pediatric Surgery, Dr. von Hauner Children's Hospital, Ludwig-Maximilian University of Munich, Munich; 4 Department of Orthopedic Trauma, Hand and Reconstructive Surgery, University Hospitals of Ulm; 5 Department of Trauma, Hand and Reconstructive Surgery, Ev. Diakonissenanstalt, Karlsruhe; 6Department of Trauma and Reconstructive Surgery, Karl-Olga-Krankenhaus, Stuttgart, Germany; 7Laboratory of Experimental Oncology, Orthopedic Rizzoli Institute, Bologna, Italy; 8Department of Orthopedics, University of Düsseldorf; 9 Institute of Pathology, University of Giessen; 10Department of Translational Brain Research, DZNE (German Center for Neurodegenerative Diseases) and Ludwig-Maximilian University of Munich, Munich; 11Department of Neurosurgery, University of Kiel, Kiel; 12Department of Trauma and Orthopedic Surgery, Diakoniekrankenhaus, Rotenburg (Wümme), Germany Received October 30, 2013; Accepted December 3, 2013 DOI: 10.3892/ijo.2014.2268 Abstract. The molecular basis of chordoma is still poorly understood, particularly with respect to differentially expressed genes involved in the primary origin of chordoma. In this study, therefore, we compared the transcriptional expression profile of one sacral chordoma recurrence, two chordoma cell lines (U-CH1 and U-CH2) and one chondrosarcoma cell line (U-CS2) with vertebral disc using a high-density oligonucleotide array. The expression of 65 genes whose mRNA levels differed significantly (p2 for 98% of the time. Based on the observations, robust changes can be identified by selecting transcripts with a fold change of >2 for increases and
