Mammalian Genome 3: $206-$219, 1992
~enome 9 Springer-VerlagNew York Inc. 1992
Mouse Chromosome 14 Joseph H. Nadeau 1'* and Roger Cox 2 1The Jackson Laboratory, Bar Harbor, Maine 04609, USA; 2Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, UK Received April 24, 1992
Introduction Since the previous report (Nadeau et al. 1991a), considerable progress has been made in the development of the genetic map for Chromosome (Chr) 14. Map density has doubled, and several new loci in the distal half of the chromosome have been identified. In this report, we reevaluate the status of genetic, cytogenetic, physical, and comparative maps.
Original description of Chr 14 loci A list of loci located on Chr 14, together with references to the original description, is provided in Table 1. Also included are 'old' gene symbols that have been replaced, recommended reference loci, genetic map localizations, locus type, methods used for assessing the phenotype, symbol and location of the human homolog, and selected mapping references.
Cytogenetic map A list of reciprocal translocations and Robertsonian translocations is provided in Fig. 1. There are no new listings or new data about these aberrations. Cytogenetic assignments are also illustrated in Fig. 1. The new assignments are Ctla-7 distal to Tcra by in situ hybridization (Jenne et al. 1991) and Rarb to band A (Mattei et al. 1991). Both localizations are consistent with linkage assignments.
Synteny assignments and somatic cell resources New synteny assignments are Cchlla2 (Chin et al. 1991), Gjb-2 (Hsieh et al. 1991b), Glud (Tzimagiorgis
*Chair of Committee for Mouse Chromosome 14
et al. 1991), Gnrh (Williamson et al. 1991), and Htr-2 (Hsieh et al. 1991a; Sparkes et al. 1991). The synteny assignments of Nfl and Tcra have been confirmed (Williamson et al. 1991). No new somatic cell resources have been reported. New data make the synteny assignment for Trp53ps ambiguous. Although somatic cell hybridization analysis suggests a location on Chr 14 (Czosnek et al., 1984), a Trp53-ps simple sequence length polymorphism shows linkage to several loci on Chr 17 in the BXD RI strains (Aitman et al. 1991). The basis for this ambiguity is uncertain.
Recombinant inbred strains The strain distribution for each Chr 14 locus that has been typed in recombinant inbred (RI) strains is listed in Table 2. Some lines appear to have double crossovers involving a single locus only. In our experience, these sometimes represent mistyping (Nadeau et al. 1991b). We recommend removal of two loci, amd (Traystman et al. 1991) and D14J! (Nadeau et al. 1992a), from the Chr 14 map. When the proper statistical test is used (Silver and Buckler 1986), the evidence for linkage to other Chr 14 in the AXB and BXA RI strains is not statistically significant.
Congenic strains A remarkable observation has been made with a strain congenic for a Chr 17 locus. B10.A(18)R is a congenic strain in which the H-2D a allele was genetically transferred from the 129/J strain to the C57BL/10 host strain. This strain also has a portion of the Tcra-W locus from 129/J (Nakajima et al. 1992). This 129derived segment does not extend to the closely linked gene Rib-1 (Fig. 2). This appears to be one of the few examples of a strain that is coincidentally congenic for
*
*
*
* * * * * * * * * * * * * * * * * * *
*
*
Aa 8
*
Esterase D (may be the same as Es-lO) Form~maidase-5 (E.C. 3.5.1.9) Withdrawn, now Glud Withdrawn, now Glud Gap junction membrane channel protein beta-2 Glutamate ddaydroge~tase (E,C. 1,4.1.3) Withdrawn, now Glud
F_.ad
&"
hph-I
Gm4J H-8 Hap hog
gm
G/ud G/ud/
Gdh-X Gjb-2
For-5
Disorganization Estcrase-10
Da Es-IO
Gonadotropin-releasing hormone (= hpg) Histocomtmtibility-8 Withdrawn, now Rarb Withdrawn, now Gm'h Hypexphenylalaninemla Hairless (= Pray-43)
Crunmetal
D N A ~graent, MIT-2
DNA segmont, MIT-3 DNA segmont, MIT-4 DNA segmont, MIT-5 DNA sesmem, MIT-6 DNA segment, M1T-7 DNA segment, M1T-8 DNA segment, MIT-9 DNA segment, Nafield Dept. of Surgery (= Plau) DNA segment,lhtsteur-I DNA segment, Pasteur-2 DNA segment, Roswell Park-4
DNA segment, Bxigham Yotmg Univeality-2 DNA segment, Btishtm Young University-3 DNA segment, Bzigham Youn$ Univc~mity-4 DNA seSment. Bril~am Young Univ~'sity-5 DNA sesmont, Elizabeth H. Lacy-I DNA se4Fnent, Jaekaon-1 DNA segnumt, M e C l ~ d - 1 DNA segmont, M/T-1
CytotoxicT-lymphocy~ ~tigen.7 D N A segment,Brigham Young Univcrsity-I
1 1
I
1
1
37.0 26.5 7.3 37.0 26.5 37.6
24.9
38.6 38.6 22.3 22.3 S 22.3 22,3
31.9 38.6
26.5 26.5 26.5 35.2 42.8 On Chr 14? 39.6 7.1 9.0 17.1 21.7 30.0 35.2 39.6 45.6 54.6 8.8 14.8 69.0 S
7.1
57.0 30.3 36.7 24.4 19.0 28.8 28.8 28.8 28.8
Withdrawn, now Tyrp-2 B lymphdd kinase Bone moqlogon prince- I Bone motghogen protein 2b-I Calcium channel, L type, 1A1 ~bunit CytotoxieT-lymphocyte antigen-1 Cytotoxie T-lymphoey~ antigen-5 Cytotoxic T-lymphocyte ~atiSen.6
28.8 S 37.6 On Chr 147
M (eM)
26.5
A
Angtogenin
Aeetyleholine receptor alpha-2 neural Adeumine k i r t l e (E.C. 2.7,1,20) Agitans (pro~bly extlnet) Autoimmune myocardial disease
Gene rumae
Dl4Byu2 Dl4Byu3 Dl4Bywr Dl4Byu5 Dl4Ehll Di4JI D14McCI D14Mitl D14Mit2 D14Mit3 DI4MiI4 Dl4Mit5 D14Mit6 Dl4Mit7 Dl4Mit8 DI4MiO D14Ndal DI4PaM Dl4Pas2 DI4Rp4
Ct/a-5 Caa-6 Caa.7 Dl4Byu, l
Blk Bmp-I Bmp2b-I CcMla2 Ctla-I
bg
Acra-2 Adk q amd
New Iamm
TaMe 1. Locus list for mouse Chr 14.
L,S R L C,L
B,D,V V D,V
L
S L,R,S
L L,R
L L,R,S
R R R R L R R L,R L,R L L L,R L,R L,R L L L R L R
R
L L L L,S I,L L L,R C,L
R
L S L R
Method
B,D,V B
V
B,D B,D
B,D B
V B,D
D D D D D D D D D D D D D D D D D D D D
B,D D
D B,D B,D B,D B,D B,D B,D
D
B,D B.D V V
T
LHRH
GJB2 GLUD
ESD
CCHLIA2 CTLA1
BMP1
ANG
ADK
H. symbol
8p21-pll.2
13
1oq23~z4
13ql 4.1-q14.2
3p26 or 3p21 14ql 1-ql 3
g
loq11424
H. location
C o n t i n u e d on next p a g e
78 10,11 4,16,20,47,67,70,86,100,101, 116, 163
40, 42, 70, 71 78, 157, 177, 187 164
82 8, 76,128, 157, 158,181
45, 94 22, 191 42, 129,191 27 8, 17, 22, 32, 76, 126, 128, 156 32, 112 32,112 32, 89,113 190 190 190 190 190 87 130 184 43 43 43 43 43 43 43 43 43 177 157 157 48 84, 85 36, 56, 95, 107, 114, 153, 172, 188,189 172 33
8, 79 107, 153 80 179 171
Refeamce
e"
t-~
7~
63
63 o
70
r
g
Z
Wc wl Wnt-SA Xmv-I 9
Xe~otropie-MCF lmkemia virus-19
Wingless-related M M T V integrationsite-5A
1
Td Toll Tad Tpi-9 Trlx$3-ps T~r#-2 Vcl
D,V
SFTPI SFTP2
RARB RB1 RBP3
PLAU
NFL NPI
MYH6
HTR2
H. symbol
10ql 1.2~ter
14ql 1.2 14ql 1.2
10q21
~enome 9 Springer-VerlagNew York Inc. 1992
Mouse Chromosome 14 Joseph H. Nadeau 1'* and Roger Cox 2 1The Jackson Laboratory, Bar Harbor, Maine 04609, USA; 2Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, UK Received April 24, 1992
Introduction Since the previous report (Nadeau et al. 1991a), considerable progress has been made in the development of the genetic map for Chromosome (Chr) 14. Map density has doubled, and several new loci in the distal half of the chromosome have been identified. In this report, we reevaluate the status of genetic, cytogenetic, physical, and comparative maps.
Original description of Chr 14 loci A list of loci located on Chr 14, together with references to the original description, is provided in Table 1. Also included are 'old' gene symbols that have been replaced, recommended reference loci, genetic map localizations, locus type, methods used for assessing the phenotype, symbol and location of the human homolog, and selected mapping references.
Cytogenetic map A list of reciprocal translocations and Robertsonian translocations is provided in Fig. 1. There are no new listings or new data about these aberrations. Cytogenetic assignments are also illustrated in Fig. 1. The new assignments are Ctla-7 distal to Tcra by in situ hybridization (Jenne et al. 1991) and Rarb to band A (Mattei et al. 1991). Both localizations are consistent with linkage assignments.
Synteny assignments and somatic cell resources New synteny assignments are Cchlla2 (Chin et al. 1991), Gjb-2 (Hsieh et al. 1991b), Glud (Tzimagiorgis
*Chair of Committee for Mouse Chromosome 14
et al. 1991), Gnrh (Williamson et al. 1991), and Htr-2 (Hsieh et al. 1991a; Sparkes et al. 1991). The synteny assignments of Nfl and Tcra have been confirmed (Williamson et al. 1991). No new somatic cell resources have been reported. New data make the synteny assignment for Trp53ps ambiguous. Although somatic cell hybridization analysis suggests a location on Chr 14 (Czosnek et al., 1984), a Trp53-ps simple sequence length polymorphism shows linkage to several loci on Chr 17 in the BXD RI strains (Aitman et al. 1991). The basis for this ambiguity is uncertain.
Recombinant inbred strains The strain distribution for each Chr 14 locus that has been typed in recombinant inbred (RI) strains is listed in Table 2. Some lines appear to have double crossovers involving a single locus only. In our experience, these sometimes represent mistyping (Nadeau et al. 1991b). We recommend removal of two loci, amd (Traystman et al. 1991) and D14J! (Nadeau et al. 1992a), from the Chr 14 map. When the proper statistical test is used (Silver and Buckler 1986), the evidence for linkage to other Chr 14 in the AXB and BXA RI strains is not statistically significant.
Congenic strains A remarkable observation has been made with a strain congenic for a Chr 17 locus. B10.A(18)R is a congenic strain in which the H-2D a allele was genetically transferred from the 129/J strain to the C57BL/10 host strain. This strain also has a portion of the Tcra-W locus from 129/J (Nakajima et al. 1992). This 129derived segment does not extend to the closely linked gene Rib-1 (Fig. 2). This appears to be one of the few examples of a strain that is coincidentally congenic for
*
*
*
* * * * * * * * * * * * * * * * * * *
*
*
Aa 8
*
Esterase D (may be the same as Es-lO) Form~maidase-5 (E.C. 3.5.1.9) Withdrawn, now Glud Withdrawn, now Glud Gap junction membrane channel protein beta-2 Glutamate ddaydroge~tase (E,C. 1,4.1.3) Withdrawn, now Glud
F_.ad
&"
hph-I
Gm4J H-8 Hap hog
gm
G/ud G/ud/
Gdh-X Gjb-2
For-5
Disorganization Estcrase-10
Da Es-IO
Gonadotropin-releasing hormone (= hpg) Histocomtmtibility-8 Withdrawn, now Rarb Withdrawn, now Gm'h Hypexphenylalaninemla Hairless (= Pray-43)
Crunmetal
D N A ~graent, MIT-2
DNA segmont, MIT-3 DNA segmont, MIT-4 DNA segmont, MIT-5 DNA sesmem, MIT-6 DNA segment, M1T-7 DNA segment, M1T-8 DNA segment, MIT-9 DNA segment, Nafield Dept. of Surgery (= Plau) DNA segment,lhtsteur-I DNA segment, Pasteur-2 DNA segment, Roswell Park-4
DNA segment, Bxigham Yotmg Univeality-2 DNA segment, Btishtm Young University-3 DNA segment, Bzigham Youn$ Univc~mity-4 DNA seSment. Bril~am Young Univ~'sity-5 DNA sesmont, Elizabeth H. Lacy-I DNA se4Fnent, Jaekaon-1 DNA segnumt, M e C l ~ d - 1 DNA segmont, M/T-1
CytotoxicT-lymphocy~ ~tigen.7 D N A segment,Brigham Young Univcrsity-I
1 1
I
1
1
37.0 26.5 7.3 37.0 26.5 37.6
24.9
38.6 38.6 22.3 22.3 S 22.3 22,3
31.9 38.6
26.5 26.5 26.5 35.2 42.8 On Chr 14? 39.6 7.1 9.0 17.1 21.7 30.0 35.2 39.6 45.6 54.6 8.8 14.8 69.0 S
7.1
57.0 30.3 36.7 24.4 19.0 28.8 28.8 28.8 28.8
Withdrawn, now Tyrp-2 B lymphdd kinase Bone moqlogon prince- I Bone motghogen protein 2b-I Calcium channel, L type, 1A1 ~bunit CytotoxieT-lymphocyte antigen-1 Cytotoxie T-lymphoey~ antigen-5 Cytotoxic T-lymphocyte ~atiSen.6
28.8 S 37.6 On Chr 147
M (eM)
26.5
A
Angtogenin
Aeetyleholine receptor alpha-2 neural Adeumine k i r t l e (E.C. 2.7,1,20) Agitans (pro~bly extlnet) Autoimmune myocardial disease
Gene rumae
Dl4Byu2 Dl4Byu3 Dl4Bywr Dl4Byu5 Dl4Ehll Di4JI D14McCI D14Mitl D14Mit2 D14Mit3 DI4MiI4 Dl4Mit5 D14Mit6 Dl4Mit7 Dl4Mit8 DI4MiO D14Ndal DI4PaM Dl4Pas2 DI4Rp4
Ct/a-5 Caa-6 Caa.7 Dl4Byu, l
Blk Bmp-I Bmp2b-I CcMla2 Ctla-I
bg
Acra-2 Adk q amd
New Iamm
TaMe 1. Locus list for mouse Chr 14.
L,S R L C,L
B,D,V V D,V
L
S L,R,S
L L,R
L L,R,S
R R R R L R R L,R L,R L L L,R L,R L,R L L L R L R
R
L L L L,S I,L L L,R C,L
R
L S L R
Method
B,D,V B
V
B,D B,D
B,D B
V B,D
D D D D D D D D D D D D D D D D D D D D
B,D D
D B,D B,D B,D B,D B,D B,D
D
B,D B.D V V
T
LHRH
GJB2 GLUD
ESD
CCHLIA2 CTLA1
BMP1
ANG
ADK
H. symbol
8p21-pll.2
13
1oq23~z4
13ql 4.1-q14.2
3p26 or 3p21 14ql 1-ql 3
g
loq11424
H. location
C o n t i n u e d on next p a g e
78 10,11 4,16,20,47,67,70,86,100,101, 116, 163
40, 42, 70, 71 78, 157, 177, 187 164
82 8, 76,128, 157, 158,181
45, 94 22, 191 42, 129,191 27 8, 17, 22, 32, 76, 126, 128, 156 32, 112 32,112 32, 89,113 190 190 190 190 190 87 130 184 43 43 43 43 43 43 43 43 43 177 157 157 48 84, 85 36, 56, 95, 107, 114, 153, 172, 188,189 172 33
8, 79 107, 153 80 179 171
Refeamce
e"
t-~
7~
63
63 o
70
r
g
Z
Wc wl Wnt-SA Xmv-I 9
Xe~otropie-MCF lmkemia virus-19
Wingless-related M M T V integrationsite-5A
1
Td Toll Tad Tpi-9 Trlx$3-ps T~r#-2 Vcl
D,V
SFTPI SFTP2
RARB RB1 RBP3
PLAU
NFL NPI
MYH6
HTR2
H. symbol
10ql 1.2~ter
14ql 1.2 14ql 1.2
10q21
