Accepted Manuscript ® LC-MS/MS profiling and neuroprotective effect of Mentat against transient global ischemia and reperfusion-induced brain injury in rats Gollapalle Lakshminarayanashasthri Viswanatha, Lakkavalli Mohan Sharath kumar, Mohamed Rafiq, Kethaganahalli Jayaramaiah Kavya, Agadi Hiremath Thippeswamy, Huvinamadu Chandrashekarappa Yuvaraj, Mohammed Azeemuddin, Suryakanth Dattatreya Anturlikar, Pralhad Sadashiv Patki, Uddagiri Venkanna Babu, Shyam Ramakrishnan PII:
S0899-9007(15)00084-2
DOI:
10.1016/j.nut.2015.02.009
Reference:
NUT 9476
To appear in:
Nutrition
Received Date: 10 August 2014 Revised Date:
2 February 2015
Accepted Date: 6 February 2015
Please cite this article as: Viswanatha GL, Sharath kumar LM, Rafiq M, Kavya KJ, Thippeswamy AH, Chandrashekarappa Yuvaraj H, Azeemuddin M, Anturlikar SD, Patki PS, Babu UV, Ramakrishnan ® S, LC-MS/MS profiling and neuroprotective effect of Mentat against transient global ischemia and reperfusion-induced brain injury in rats, Nutrition (2015), doi: 10.1016/j.nut.2015.02.009. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
RI PT
Neurological Deficit score Memory Impairment Motor activity Transient
M AN U
SC
Ischemia/ Reperfusion
TE D
Infarct Size Cerebral edema Lipid peroxidation
EP AC C
In vitro antioxidant
7 Days Pretreatment Mentat Granules In vivo Evaluation
DPPH Assay– Assay– 52.6 ± 4.6 (IC50 value) FRAP Assay –14064 ± 895.3 (FRAP value) ORAC assay –615 ± 52.5 (ORAC value)
Histological Damage
ACCEPTED MANUSCRIPT
LC-MS/MS profiling and neuroprotective effect of Mentat® against transient global ischemia and reperfusion-induced brain injury in rats
RI PT
Gollapalle Lakshminarayanashasthri Viswanatha1, Lakkavalli Mohan Sharath kumar2, Mohamed Rafiq1*, Kethaganahalli Jayaramaiah Kavya1, Agadi Hiremath Thippeswamy1, Huvinamadu Chandrashekarappa Yuvaraj1, Mohammed
SC
Azeemuddin1, Suryakanth Dattatreya Anturlikar1, Pralhad Sadashiv Patki3,
1
2
3
M AN U
Uddagiri Venkanna Babu2, Shyam Ramakrishnan4
Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India
Department of Phytochemistry, R&D Center, The Himalaya Drug Company, Bangalore, India
Medical Services and Clinical Trials, R&D Center, The Himalaya Drug Company, Bangalore, India Chief Scientific Officer, The Himalaya Drug Company, Bangalore, India
EP
TE D
4
AC C
*Address for correspondence: Dr. Mohamed Rafiq Department of Pharmacology, R&D Center, The Himalaya Drug Company, Makali, Bangalore 562 162, Karnataka, India Email: [email protected] Mob: +91 9844108957 Phone no.: +91 8023714444 Fax: +91 8023714471
1
ACCEPTED MANUSCRIPT
LC-MS/MS profiling and neuroprotective effect of Mentat® against
Abstract
RI PT
transient global ischemia and reperfusion-induced brain injury in rats
Objective: This study was designed to evaluate the possible beneficial effect of Mentat against transient global ischemia-induced brain injury in rats.
SC
Methods: The Neuroprotective effect of Mentat was evaluated against transient global ischemia and reperfusion-induced brain injury in rats. Various neurobehavioral and
M AN U
biochemical parameters were carried out, followed by morphological and histopathological evaluation of brain tissue was performed to conclude the protective effect of Mentat. In addition, in vitro antioxidant assays were performed to explore the antioxidant capacity of Mentat and detailed Liquid Chromatography-Mass Spectrometry (LC-MS/MS) profiling was
Mentat.
TE D
carried out to identify the active phytoconstituents responsible for the protective effect of
Results: Sixty minutes of transient global ischemia followed by 24 h reperfusion (I/R) caused significant alterations in the cognitive and neurological functions in the ischemia control
EP
group (p
S0899-9007(15)00084-2
DOI:
10.1016/j.nut.2015.02.009
Reference:
NUT 9476
To appear in:
Nutrition
Received Date: 10 August 2014 Revised Date:
2 February 2015
Accepted Date: 6 February 2015
Please cite this article as: Viswanatha GL, Sharath kumar LM, Rafiq M, Kavya KJ, Thippeswamy AH, Chandrashekarappa Yuvaraj H, Azeemuddin M, Anturlikar SD, Patki PS, Babu UV, Ramakrishnan ® S, LC-MS/MS profiling and neuroprotective effect of Mentat against transient global ischemia and reperfusion-induced brain injury in rats, Nutrition (2015), doi: 10.1016/j.nut.2015.02.009. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
RI PT
Neurological Deficit score Memory Impairment Motor activity Transient
M AN U
SC
Ischemia/ Reperfusion
TE D
Infarct Size Cerebral edema Lipid peroxidation
EP AC C
In vitro antioxidant
7 Days Pretreatment Mentat Granules In vivo Evaluation
DPPH Assay– Assay– 52.6 ± 4.6 (IC50 value) FRAP Assay –14064 ± 895.3 (FRAP value) ORAC assay –615 ± 52.5 (ORAC value)
Histological Damage
ACCEPTED MANUSCRIPT
LC-MS/MS profiling and neuroprotective effect of Mentat® against transient global ischemia and reperfusion-induced brain injury in rats
RI PT
Gollapalle Lakshminarayanashasthri Viswanatha1, Lakkavalli Mohan Sharath kumar2, Mohamed Rafiq1*, Kethaganahalli Jayaramaiah Kavya1, Agadi Hiremath Thippeswamy1, Huvinamadu Chandrashekarappa Yuvaraj1, Mohammed
SC
Azeemuddin1, Suryakanth Dattatreya Anturlikar1, Pralhad Sadashiv Patki3,
1
2
3
M AN U
Uddagiri Venkanna Babu2, Shyam Ramakrishnan4
Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India
Department of Phytochemistry, R&D Center, The Himalaya Drug Company, Bangalore, India
Medical Services and Clinical Trials, R&D Center, The Himalaya Drug Company, Bangalore, India Chief Scientific Officer, The Himalaya Drug Company, Bangalore, India
EP
TE D
4
AC C
*Address for correspondence: Dr. Mohamed Rafiq Department of Pharmacology, R&D Center, The Himalaya Drug Company, Makali, Bangalore 562 162, Karnataka, India Email: [email protected] Mob: +91 9844108957 Phone no.: +91 8023714444 Fax: +91 8023714471
1
ACCEPTED MANUSCRIPT
LC-MS/MS profiling and neuroprotective effect of Mentat® against
Abstract
RI PT
transient global ischemia and reperfusion-induced brain injury in rats
Objective: This study was designed to evaluate the possible beneficial effect of Mentat against transient global ischemia-induced brain injury in rats.
SC
Methods: The Neuroprotective effect of Mentat was evaluated against transient global ischemia and reperfusion-induced brain injury in rats. Various neurobehavioral and
M AN U
biochemical parameters were carried out, followed by morphological and histopathological evaluation of brain tissue was performed to conclude the protective effect of Mentat. In addition, in vitro antioxidant assays were performed to explore the antioxidant capacity of Mentat and detailed Liquid Chromatography-Mass Spectrometry (LC-MS/MS) profiling was
Mentat.
TE D
carried out to identify the active phytoconstituents responsible for the protective effect of
Results: Sixty minutes of transient global ischemia followed by 24 h reperfusion (I/R) caused significant alterations in the cognitive and neurological functions in the ischemia control
EP
group (p
