©) 1990 Oxford University Press

Nucleic Acids Research, Vol. 18, No. 7 1907

Murine polyubiquitin mRNA

sequence

J.S.Finch*, K.Bonham, P.Krieg and G.T.Bowden Department of Radiation Oncology, University of Arizona Medical School, Tucson, AZ 85724, USA Submitted February 16, 1990

EMBL accession no. X51703

A cDNA clone, p-mal-3, was isolated from a squamous cell carcinoma library based on its overexpression in mouse skin papillomas and squamous cell carcinomas compared to normal epidermis (1). Using an oligonucleotide to the 3' end of p-mal-3 as a probe, a nearly full length cDNA clone of this message was isolated from a XgtlO library made from the transformed murine epidermal cell line PDV-C57 (2). Sequencing revealed a predicted amino acid sequence of four tandem repeats of the conserved protein ubiquitin (3). The clone 3JSF includes 62 5' untranslated bases, the four ubiquitin tandem repeats, 152 3' untranslated bases including the polyadenylation signal, and a poly A tail of at least 35 bases. 34 more 5' untranslated bases were determined by primer extension to within 1-2 bases of the cap site. Independently, another clone of this cDNA was isolated from

a lymphocyte library (Dr. Thomas St. John, Fred Hutchinson Cancer Research Center, Personal Communication).

ACKNOWLEDGEMENT Research was supported by USPHS Grant CA40584.

REFERENCES 1. Melber,K., Krieg,P., Furstenberger,G. and Marks,F. (1986) Carcinogenesis 7, 317-322. 2. Fusenig,N.E., Samsel,W., Thon,W. and Worst,P.K.M. (1973) INSERM 19,

219-228. 3. Schlesinger,D.H., Goldstein,G. and Niall,H.D. (1975b) Biochemistry 14, 2214-2218.

Polyubiquitin mRNA 96 bp 5'

228 bp Coding I

228 bp Codn I

152 bp 44 bP 13'Untransl. AAA

231 bp

228 bp

CodingII

Coding IV

-96 TNACGAGAGGCT

-62 TTGTCCGGTTCGGCGGTCTTTCTGTGAGGG

TGTGAGGGTGTTTCGACGCGCTGGGCGGTTTGTGCTTTCATCACATTTGTTAACAGGTCAAA 1

ATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCC T K V P M Q F L T G K I T T L E V E E N V K A I S D T I AAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTC I O E K G I K I D R L F A G A D K L E D G R T Q 0 P P Q TCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGC S D N Y I Q K E S T L H L V L R L R G G 229 ATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCCTGGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCC F M Q I E N V K A V K T L T G K T I T L E V E P S D T I AAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTC K R T I Q D K E G I P P D Q Q R L I F A G K L E D G A TCTGATTACAACATCCAGAAAGAGTCAACCCTGCACCTGGTCCTCCGTCTGAGGGGTGGC S D Y N I K T L H L V L R L G *E S R G Q 457 ATGCAGATCTTCGTGAAGACCCTGACTGGCAAGACCATCACCCTGGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCC T M Q F V K T K T T L E D I E N I L T G I V K A V E P S AAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAACAGCTGGAAGATGGCCGCACCCTC K I Q D K E G I P P D Q Q R L I F A G K Q L E D G R T A TCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGC S D N Y I Q K E S T L H L V L R L R G G 685

ATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCTTGGAGGTGGAGCCCAGTGACACCATCGAGAATGTGAAGGCC M

Q

I

F

V

K

T

L

T

G

K

T

I

T

L

E

V

E

P

S

D

T

I

E

N

V

K

A

AAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTAGAAGATGGCCGCACTCTC I 0 K K E D G I P P D Q Q R L I F A G K Q L TCTGATTACAACATCCAGAAAGAGTCGACCCTGCACCTGGTCCTCCGTCTGAGGGGTGGCTAT D S Y N I Q K E S T L H L V L R L R G G 916

E

D

G

R

T

A

TAATTATTCGGTCTGCATTCCCAGTGGGCAGTGATGGCATTACTCTGCACTCTAGCCACTTGCCCCAATTTAAGTTTAGAAATT ACAAGTTTCAATAATAGCTGAACCTCTGTTAAAAAATGTTAATAAAGGTTTTGTTGCATGGTAAGCATAAAA

*

To whom correspondence should be addressed