C a se Report Acta Haematol 1992;87:153-155
M.P. Chowa K.J. Sunb C.H. Yunga H.Y. Hu ' J.L. Tzeng3 T.D. Leec
Neonatal Alloimmune Thrombocytopenia Due to HLA-A2 Antibody
Blood Bank, Department of Medicine, and Department of Pediatrics, Veterans General Hospital, Taipei, Republic of China; American Red Cross Blood Services, Holland Laboratory, Rockville, Md.. USA
Key Words
Abstract
HLA antibody Neonatal alloimmune thrombocytopenia Platelet alloantibody
A male, full-term baby with thrombocytopenia was born by a G3P2A1 mother who was not associated with autoimmune disease. Platelet antibody screening was positive by using lymphocytotoxicity test, platelet suspension immunofluo rescence test and solid-phase red cell adherence test. The identified HLA anti body was of A2 specificity. It was confirmed by testing the m other’s and the ba by’s sera against the lymphocytes and platelets of 10 HLA-A2-positive donors. The possibility of platelet-specific antibody as the cause of neonatal alloimmune thrombocytopenia was ruled out by testing against platelets of 10 HLAA2-negative donors and the known platelet-specific antigens utilizing immobi lized, purified platelet glycoprotein as targets. The mother’s serum reacted strongly with both the father’s and the baby’s platelets and lymphocytes. This neonatal thrombocytopenia was most likely due to the maternal HLA anti body, which was induced by her antecedent gestations.
Introduction
Case Report
Received: December 31,1990 Accepted: October 8. 1991
A male baby of 15 days was referred to our hospital on account of thrombocytopenia. He was the 2nd child of a mother with a gestation history of G3P2A1. He was full term with vacuum delivery. His birth weight was 3.2 kg with an immediate Apgar score of 8. The mother was of good health with no history of previous transfusion, medica tion or autoimmune disorder. Her platelet count was normal. The pa tient’s sister had no thrombocytopenia. Physical examination re vealed jaundice and cephalohcmatoma. No purpura or ecchymosis was noted. He remained afebrile without evidence of sepsis. The blood groups of the baby and his parents were ‘A’. Complete blood count showed: Hb 15.6 g/dl, WBC 3.8 x 10‘Vl, and platelet count 81 x 1071. Prothrombin time was 150 sand partial thromboplastin time
Mo-Ping Chow, MD Blood Bank. Department of Medicine Veterans General 1lospital-Taipci Shih-pai Taipei, Taiwan (Republic of China)
© 1992 S. Karger AG, Basel
M.P. Chowa K.J. Sunb C.H. Yunga H.Y. Hu ' J.L. Tzeng3 T.D. Leec
Neonatal Alloimmune Thrombocytopenia Due to HLA-A2 Antibody
Blood Bank, Department of Medicine, and Department of Pediatrics, Veterans General Hospital, Taipei, Republic of China; American Red Cross Blood Services, Holland Laboratory, Rockville, Md.. USA
Key Words
Abstract
HLA antibody Neonatal alloimmune thrombocytopenia Platelet alloantibody
A male, full-term baby with thrombocytopenia was born by a G3P2A1 mother who was not associated with autoimmune disease. Platelet antibody screening was positive by using lymphocytotoxicity test, platelet suspension immunofluo rescence test and solid-phase red cell adherence test. The identified HLA anti body was of A2 specificity. It was confirmed by testing the m other’s and the ba by’s sera against the lymphocytes and platelets of 10 HLA-A2-positive donors. The possibility of platelet-specific antibody as the cause of neonatal alloimmune thrombocytopenia was ruled out by testing against platelets of 10 HLAA2-negative donors and the known platelet-specific antigens utilizing immobi lized, purified platelet glycoprotein as targets. The mother’s serum reacted strongly with both the father’s and the baby’s platelets and lymphocytes. This neonatal thrombocytopenia was most likely due to the maternal HLA anti body, which was induced by her antecedent gestations.
Introduction
Case Report
Received: December 31,1990 Accepted: October 8. 1991
A male baby of 15 days was referred to our hospital on account of thrombocytopenia. He was the 2nd child of a mother with a gestation history of G3P2A1. He was full term with vacuum delivery. His birth weight was 3.2 kg with an immediate Apgar score of 8. The mother was of good health with no history of previous transfusion, medica tion or autoimmune disorder. Her platelet count was normal. The pa tient’s sister had no thrombocytopenia. Physical examination re vealed jaundice and cephalohcmatoma. No purpura or ecchymosis was noted. He remained afebrile without evidence of sepsis. The blood groups of the baby and his parents were ‘A’. Complete blood count showed: Hb 15.6 g/dl, WBC 3.8 x 10‘Vl, and platelet count 81 x 1071. Prothrombin time was 150 sand partial thromboplastin time
Mo-Ping Chow, MD Blood Bank. Department of Medicine Veterans General 1lospital-Taipci Shih-pai Taipei, Taiwan (Republic of China)
© 1992 S. Karger AG, Basel
