Original Article

Noncoding RNA small nucleolar RNA host gene 1 promote cell proliferation in nonsmall cell lung cancer You J§, Fang N§, Gu J, Zhang Y, Li X, Zu L, Zhou Q Tianjin Key Labotatory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China §

You J and Fang N contributed equally to this work

Correspondence to: Prof. You Ja, E-mail: [email protected], Prof. Zhou Qb, E-mail: [email protected]

Abstract

BACKGROUND: Nonsmall cell lung cancer (NSCLC) is the major cause of cancer death worldwide. Increasing evidence shows that noncoding

RNAs (ncRNAs) are widely involved in the development and progression of NSCLC. ncRNA small nucleolar RNA host gene 1 (SNHG1) has not been studied in cancer, especially its role in lung cancer remains unknown. Our studies were designed to investigate the expression and biological significance of SNHG1 in lung cancer. SNHG1 may be a novel ncRNA in early diagnosis in lung cancer. METHODS: Noncoding RNA SNHG1 expression in 7 lung cancer cell lines was measured by quantitative real‑time polymerase chain reaction. RNA interference approaches were used to find the biological functions of SNHG1. The effect of SNHG1 on proliferation was evaluated by cell count and crystal violet stains. RESULTS: Noncoding RNA SNHG1 expression was significantly upregulated in lung cancer cells when compared with normal bronchial epithelial cells. In addition, in vitro assays our results indicated that knockdown of SNHG1 inhibited cell proliferation. CONCLUSIONS: Our data indicated that ncRNA SNHG1 is significantly upregulated in NSCLC cell lines and may represent a new biomarker and a potential therapeutic target for NSCLC intervention. Key Words: Nonsmall cell lung cancer, proliferation, small nucleolar RNA host gene 1

Introduction Lung cancer is the most common cause of cancer death, and the majority of lung cancer cases are nonsmall cell lung cancer (NSCLC), which accounts for approximately 80% of lung cancer. [1,2] Though the enormous improvements made in chemotherapy and radiotherapy over the past few decades, the prognosis of lung cancer is unfavorable. [3] The 5‑year overall survival rate is still 

Noncoding RNA small nucleolar RNA host gene 1 promote cell proliferation in nonsmall cell lung cancer.

Nonsmall cell lung cancer (NSCLC) is the major cause of cancer death worldwide. Increasing evidence shows that noncoding RNAs (ncRNAs) are widely invo...
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