Journal of Periodontology; Copyright 2015
DOI: 10.1902/jop.2015.140528
Novel Coating of Surgical Suture Confers Antimicrobial Activity Against Porphyromonas Gingivalis and Enterococcus Faecalis Mohamed M Meghil1, Frederick Rueggeberg2, Ahmed EL-Awady5, Brodie Miles3, Franklin Tay4, David Pashley1, and Christopher W Cutler5 1 2 3
Department of Oral Biology, Georgia Regents University, Augusta, GA.
Department of Oral Rehabilitation, Georgia Regents University, Augusta, GA.
Division of Infectious Diseases, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO. 4
Department of Endodontics, Georgia Regents University, Augusta, GA.
5
Department of Periodontics, Georgia Regents University, Augusta, GA.
Background. The oral cavity is colonized by over 109 bacteria, many of which can increase heart disease risk when seeded into the bloodstream. Most dentoalveolar surgeries require the use of surgical sutures. Suture placement and removal can increase risk of postoperative infection and bacteremia. The aim of this study was to evaluate the antimicrobial activity of a novel quaternary ammonium compound, K21, when coated on different suture materials. Methods. The periodontal pathogen Porphyromonas gingivalis and the endodontic species Enterococcus faecalis were grown to early log phase and inoculated on enriched Brucella blood agar, on which were placed identical lengths of surgical suture (Chromic Gut, Ethibond Excel, Silk, and Ethilon [Ethicon, Inc.]) and control unwaxed dental floss impregnated with K21 at 5%, 10%, 20% and 25% v/v in ethanol vehicle. Controls included: sutures treated with vehicle, untreated sutures and unwaxed floss. Zones of inhibition in mm were measured at five randomized sites per suture/floss for each concentration and material used. Means + standard deviations of zones of inhibition were calculated and analysis of variance (ANOVA, p
DOI: 10.1902/jop.2015.140528
Novel Coating of Surgical Suture Confers Antimicrobial Activity Against Porphyromonas Gingivalis and Enterococcus Faecalis Mohamed M Meghil1, Frederick Rueggeberg2, Ahmed EL-Awady5, Brodie Miles3, Franklin Tay4, David Pashley1, and Christopher W Cutler5 1 2 3
Department of Oral Biology, Georgia Regents University, Augusta, GA.
Department of Oral Rehabilitation, Georgia Regents University, Augusta, GA.
Division of Infectious Diseases, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO. 4
Department of Endodontics, Georgia Regents University, Augusta, GA.
5
Department of Periodontics, Georgia Regents University, Augusta, GA.
Background. The oral cavity is colonized by over 109 bacteria, many of which can increase heart disease risk when seeded into the bloodstream. Most dentoalveolar surgeries require the use of surgical sutures. Suture placement and removal can increase risk of postoperative infection and bacteremia. The aim of this study was to evaluate the antimicrobial activity of a novel quaternary ammonium compound, K21, when coated on different suture materials. Methods. The periodontal pathogen Porphyromonas gingivalis and the endodontic species Enterococcus faecalis were grown to early log phase and inoculated on enriched Brucella blood agar, on which were placed identical lengths of surgical suture (Chromic Gut, Ethibond Excel, Silk, and Ethilon [Ethicon, Inc.]) and control unwaxed dental floss impregnated with K21 at 5%, 10%, 20% and 25% v/v in ethanol vehicle. Controls included: sutures treated with vehicle, untreated sutures and unwaxed floss. Zones of inhibition in mm were measured at five randomized sites per suture/floss for each concentration and material used. Means + standard deviations of zones of inhibition were calculated and analysis of variance (ANOVA, p
