1904 Nucleic Acids Research, Vol. 18, No. 7
*@
%.. 1990 Oxford University Press
Nucleotide and encoded amino acid sequences of the capsid protein gene of three dengue-2 viruses isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome or dengue fever S.Samuel, C.L.Koh', T.Pang and S.K.Lam Department of Medical Microbiology and 'Department of Genetics and Cellular Biology, University of Malaya, 59100 Kuala Lumpur, Malaysia Submitted February 20, 1990
EMBL accession nos X51708, X51709, X51710
ACKNOWLEDGEMENTS
The nucleotide sequences encoding the capsid protein of three dengue-2 (DEN-2) viruses, Ml, M2 and M3, isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome and dengue fever, respectively, were determined by direct RNA sequencing by primer extension with pentadecamer primers and the dideoxyribonucleotide chain termination method. The primers were based on the published nucleotide sequence of DEN-2 (Jamaica) (Deubel et al., 1986).
WHO Regional Office for the Western Pacific, Philippines; International Development Research Centre, Canada; Ministry of Science, Technology and Environment, Malaysia; and University of Malaysia.
REFERENCE 1. Deubel,V. et al. (1986) Virology 155, 365-377.
1 AUGAAUGACCAACGAAAAAA GGCGAGAAGUACGCCUUUCA AUAUGCUGAA M1 M2 U-.- --AA-es.-.*..o**.-. M3 51 GCGCGAGAGAAACCGCGUGU CAACUGUACAACAGCUGACA AAGAGAUUCU M1 o*eoo--Go99o-Uooo- *@@@o*s@@o A............... M2 --.... *GM3 M1 101 CCCUUGGAAUGCUGCAAGGG CGAGGACCAUUAAAACUGUU CAUGGCGCUU *A.*vs@@A M2 &* ..... @ .*X*c..U.* M3 M1 151 GUGGCGUUCCUUCGUUUCCU AACAAUCCCACCAACAGCAG GGAUACUGAA 009** M2 .XX** .*e*vv---*-**---*v-M3 m1 201 AAGAUGGGGAACGAUCAAAA AAUCAAAAGCUAUCAAUGUU UUGAGAGGGU 0000-0*0.0 M2 .G ..*U.##...oo M3 M1 251 UCAGGAAAGAGAUUGGAAGG AUGCUGAACAUCUUAAAUAG GAGACGCAGG ...... ...... M2 G M3 M1 301 ACAGCAGGCGUGAUUAUUAU GUUGAUUCCAACAGUGAUGG CG M2 . . . ***A
..
*-
0@
*s**-*@-@
A
...
*
seat*** ...
***vvvv.....
M3
...
*
...**....... 0
00 0
C*0
* ..
.
**.
***
............................
..o
0
*
*
-
.... -***... ....
..
**@*so-*
..
The three nucleotide sequences encode the following amino acid sequences:
Ml M2 M3
Ml
1 MNDQRKKARSTPFNMLKRER NRVSTVQQLTKRFSLGMLQG RGPLKLFMAL
. ..LNI....LN ..............TKKNRRI *-N Nl K 51 VAFLRFLTIPPTAGILKRWG TIKKSKAINVLRGFRKEIGR MLNILMRRRR
M2
143
M1 M2 M3
101 TAGVIIMLIPTVMA . . .1414
.
...
*es ............
0........... o
*.*@s-
*@
%.. 1990 Oxford University Press
Nucleotide and encoded amino acid sequences of the capsid protein gene of three dengue-2 viruses isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome or dengue fever S.Samuel, C.L.Koh', T.Pang and S.K.Lam Department of Medical Microbiology and 'Department of Genetics and Cellular Biology, University of Malaya, 59100 Kuala Lumpur, Malaysia Submitted February 20, 1990
EMBL accession nos X51708, X51709, X51710
ACKNOWLEDGEMENTS
The nucleotide sequences encoding the capsid protein of three dengue-2 (DEN-2) viruses, Ml, M2 and M3, isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome and dengue fever, respectively, were determined by direct RNA sequencing by primer extension with pentadecamer primers and the dideoxyribonucleotide chain termination method. The primers were based on the published nucleotide sequence of DEN-2 (Jamaica) (Deubel et al., 1986).
WHO Regional Office for the Western Pacific, Philippines; International Development Research Centre, Canada; Ministry of Science, Technology and Environment, Malaysia; and University of Malaysia.
REFERENCE 1. Deubel,V. et al. (1986) Virology 155, 365-377.
1 AUGAAUGACCAACGAAAAAA GGCGAGAAGUACGCCUUUCA AUAUGCUGAA M1 M2 U-.- --AA-es.-.*..o**.-. M3 51 GCGCGAGAGAAACCGCGUGU CAACUGUACAACAGCUGACA AAGAGAUUCU M1 o*eoo--Go99o-Uooo- *@@@o*s@@o A............... M2 --.... *GM3 M1 101 CCCUUGGAAUGCUGCAAGGG CGAGGACCAUUAAAACUGUU CAUGGCGCUU *A.*vs@@A M2 &* ..... @ .*X*c..U.* M3 M1 151 GUGGCGUUCCUUCGUUUCCU AACAAUCCCACCAACAGCAG GGAUACUGAA 009** M2 .XX** .*e*vv---*-**---*v-M3 m1 201 AAGAUGGGGAACGAUCAAAA AAUCAAAAGCUAUCAAUGUU UUGAGAGGGU 0000-0*0.0 M2 .G ..*U.##...oo M3 M1 251 UCAGGAAAGAGAUUGGAAGG AUGCUGAACAUCUUAAAUAG GAGACGCAGG ...... ...... M2 G M3 M1 301 ACAGCAGGCGUGAUUAUUAU GUUGAUUCCAACAGUGAUGG CG M2 . . . ***A
..
*-
0@
*s**-*@-@
A
...
*
seat*** ...
***vvvv.....
M3
...
*
...**....... 0
00 0
C*0
* ..
.
**.
***
............................
..o
0
*
*
-
.... -***... ....
..
**@*so-*
..
The three nucleotide sequences encode the following amino acid sequences:
Ml M2 M3
Ml
1 MNDQRKKARSTPFNMLKRER NRVSTVQQLTKRFSLGMLQG RGPLKLFMAL
. ..LNI....LN ..............TKKNRRI *-N Nl K 51 VAFLRFLTIPPTAGILKRWG TIKKSKAINVLRGFRKEIGR MLNILMRRRR
M2
143
M1 M2 M3
101 TAGVIIMLIPTVMA . . .1414
.
...
*es ............
0........... o
*.*@s-
