Nucleic Acids Research, Vol. 18, No. 11 3399
Nucleotide cDNA and complete deduced amino acid sequence of a Trypanosoma cruzi ribosomal P protein (P-JL5) Alejandro G.Schijman, Nelson J.Dusetti, Martin P.Vazquez, Sonia Lafon, Patricia Levy-Yeyati and Mariano J.Levin* Instituto de Investigaciones en Ingenieri'a Gen6tica y Biologi'a Molecular (INGEBI-CONICET), Obligado 2490, 1428 Buenos Aires, Argentina Submitted April 26, 1990
EMBL accession no. X52323
The isolation of a T. cruzi Xgtl 1 recombinant cDNA clone, JL5, which reacted predominantly with chronic Chagas heart disease sera has been reported (1). The nucleotide and deduced amino acid sequence allowed identification as the C-terminal portion of a T. cruzi ribosomal P protein (1, 2). Radioactive JL5 cDNA probes were used to screen a T. cruzi Tul2 stock epimastigote cDNA library. A recombinant was isolated, P-JL5, that encodes the complete amino acid sequence of the JL5 P protein. The underlined 17 bases at the 5' end represent the 3' region of the mini-exon sequence. The ORF of 107 amino acids begins at position 30. The first 7 N-terminal residues are 100% homologous to the yeast A2 P protein (3). The last 10 C-terminal residues define the distinctive consensus P protein motif. The serine at position 84 differs from the glycine reported for the JL5 sequence (1), due to the nucleotide change A(P-JL5) to
G(JL5).
ACKNOWLEDGEMENTS French-Argentinian Cooperation Program (INSERMCONICET); Regional Program of Biotechnology for Latin America and the Caribbean (UNDP/UNESCO); UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases; SECyT (PNB) and CONICET (Argentina).
REFERENCES 1. Levin,M.J., Mesri,E., Benarous,R., Levitus,G., Schijman,A., LevyYeyati,P., Chiale,P., Ruiz,A., Kahn,A., Rosenbaum,M., Torres,H.N. and Segura,E. (1989) Am. J. Trop. Med. Hyg. 41, 530-539. 2. Liljas,A., Thirup,S. and Matheson,A.T. (1986) Chemica Scripta 26B, 109-119. 3. Mitsui,K. and Tsurugi,K. (1988) Nucl. Acids Res. 16, 3575.
cDNA NUCLEOTIDE SEQUENCE: 1 61 121 181 241 301 361 421
GTTTCTGTAC GCCTCTCAGG TTCCTGTCGA ATACGGTATG CCACCGCTTC CAGCTGCGGA TGCTTATTTT AAAAAAAAAA
TATATTGTTG AGGGACCCCC TCCTTCTCGC CACTGAAGGC TGCACCCACT AGAGGAGGAG ATTTTATTTT AAAA
TCTTGTCAGA TCAAAGTCTG GTGGATGCAC AAATCGAAGC GCCGCTGCCG
TGAAGTACCT CAGTTGAGGC TTTTTGCCGA TTGTAGGAGG CCGCCTCTAG GACGATGACA TGGGATTTGG TGATTTTGTT TCTGTGTTGT
TGCCGCTTAC CGTATTGAAG GTTTGCAGGC GGTTACACGT TGGTGCCGCC TCTTTTTGAC CCATGAAGAG
GCTCTTGTTG GCTGCCGGCG AAGGACTTTG CCCAATGCTG GCGCCTGCTG TAAAGACAAA GCGCTAAAAA
COMPLETE DEDUCED AMINO ACID SEQUENCE:
1 61
*
MKYLAAYALV GLSGGTPSKS AVEAVLKAAG VPVDPSRVDA LFAEFAGKDF DTVCTEGKSK LVGGVTRPNA ATASAPTAAA AASSGAAAPA AAAEEEEDDD MGFGLFD
To whom correspondence should be addressed
Nucleotide cDNA and complete deduced amino acid sequence of a Trypanosoma cruzi ribosomal P protein (P-JL5) Alejandro G.Schijman, Nelson J.Dusetti, Martin P.Vazquez, Sonia Lafon, Patricia Levy-Yeyati and Mariano J.Levin* Instituto de Investigaciones en Ingenieri'a Gen6tica y Biologi'a Molecular (INGEBI-CONICET), Obligado 2490, 1428 Buenos Aires, Argentina Submitted April 26, 1990
EMBL accession no. X52323
The isolation of a T. cruzi Xgtl 1 recombinant cDNA clone, JL5, which reacted predominantly with chronic Chagas heart disease sera has been reported (1). The nucleotide and deduced amino acid sequence allowed identification as the C-terminal portion of a T. cruzi ribosomal P protein (1, 2). Radioactive JL5 cDNA probes were used to screen a T. cruzi Tul2 stock epimastigote cDNA library. A recombinant was isolated, P-JL5, that encodes the complete amino acid sequence of the JL5 P protein. The underlined 17 bases at the 5' end represent the 3' region of the mini-exon sequence. The ORF of 107 amino acids begins at position 30. The first 7 N-terminal residues are 100% homologous to the yeast A2 P protein (3). The last 10 C-terminal residues define the distinctive consensus P protein motif. The serine at position 84 differs from the glycine reported for the JL5 sequence (1), due to the nucleotide change A(P-JL5) to
G(JL5).
ACKNOWLEDGEMENTS French-Argentinian Cooperation Program (INSERMCONICET); Regional Program of Biotechnology for Latin America and the Caribbean (UNDP/UNESCO); UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases; SECyT (PNB) and CONICET (Argentina).
REFERENCES 1. Levin,M.J., Mesri,E., Benarous,R., Levitus,G., Schijman,A., LevyYeyati,P., Chiale,P., Ruiz,A., Kahn,A., Rosenbaum,M., Torres,H.N. and Segura,E. (1989) Am. J. Trop. Med. Hyg. 41, 530-539. 2. Liljas,A., Thirup,S. and Matheson,A.T. (1986) Chemica Scripta 26B, 109-119. 3. Mitsui,K. and Tsurugi,K. (1988) Nucl. Acids Res. 16, 3575.
cDNA NUCLEOTIDE SEQUENCE: 1 61 121 181 241 301 361 421
GTTTCTGTAC GCCTCTCAGG TTCCTGTCGA ATACGGTATG CCACCGCTTC CAGCTGCGGA TGCTTATTTT AAAAAAAAAA
TATATTGTTG AGGGACCCCC TCCTTCTCGC CACTGAAGGC TGCACCCACT AGAGGAGGAG ATTTTATTTT AAAA
TCTTGTCAGA TCAAAGTCTG GTGGATGCAC AAATCGAAGC GCCGCTGCCG
TGAAGTACCT CAGTTGAGGC TTTTTGCCGA TTGTAGGAGG CCGCCTCTAG GACGATGACA TGGGATTTGG TGATTTTGTT TCTGTGTTGT
TGCCGCTTAC CGTATTGAAG GTTTGCAGGC GGTTACACGT TGGTGCCGCC TCTTTTTGAC CCATGAAGAG
GCTCTTGTTG GCTGCCGGCG AAGGACTTTG CCCAATGCTG GCGCCTGCTG TAAAGACAAA GCGCTAAAAA
COMPLETE DEDUCED AMINO ACID SEQUENCE:
1 61
*
MKYLAAYALV GLSGGTPSKS AVEAVLKAAG VPVDPSRVDA LFAEFAGKDF DTVCTEGKSK LVGGVTRPNA ATASAPTAAA AASSGAAAPA AAAEEEEDDD MGFGLFD
To whom correspondence should be addressed
