680 Nucleic Acids Research, Vol. 18, No. 3
Nucleotide sequence of a cDNA clone of Xenopus platelet-derived growth factor A-chain Bruce E.Bejcek, Dean Y.Li and Thomas F.Deuel* Jewish Hospital of St Louis, 216 S. Kingshighway, St Louis, MO 63110, USA Submitted December 12, 1989
The nucleotide sequence of a cDNA clone homologous to the A-chain of the human platelet derived growth factor and isolated from a Xenopus oocyte cDNA library (1) is presented. The leader peptide is coded for by nucleotides 14-79, prepro sequences are coded for by nucleotides 80-286 and the mature protein is coded for by nucleotides 287-658. This clone is homologous
1 GCAGCAGGAC GCAATGAGGA TCTGGGCTTG 61 CCTGTCTCCT TCATTGGGCG AGGAAGCAGA 121 TCACAGCGAA ATCCGCAGCA TCAGCGACCT 181 AGGAGGCGAG GATGCTTCTG CAGCCAACAT 241 TAGGCTGGTG CCAGAAAAGC GCTCCGTTCC 301 AGTCCCTGCT ATCTGTAAAA CAAGAACTGT 361 TCCAACATCT GCCAATTTTC TGATCTGGCC 421 ATGCTGCAAC ACCAGCAGTG TCAAGTGCCA 481 GGTGGCAAAA GTAGAATATG TAAGAAAGAA 541 AGAGGAACAT CTGGAATGCA CGTGTACAGC 601 AACTGGAAGG ACTAGGGAAA CAGGTAAAAA 661 ATAACCGTCT GGAAAGTAAT TTTGAACGGC 721 AATGGAAAAA AGGCATTGAG CAGATCCAAA 781 TTTATGGTTT ATGGTTTATT TTCATGATAT 841 TTTGGTCAGC TGGAAAATCA AATTCTGTTG
*
To whom correspondence should be addressed
EMBL accession no. X17545
to the long form previously described (2) but it is missing a 33 nucleotide insertion beginning at nucleotide 604.
REFERENCES 1. Rabagliati,M.R. and Melton,D.A. (1987) Cell 48, 599-605. 2. Mercola,M., Melton,D.A. and Stiles,C.D. (1988) Science 243, 800-804.
GATTCTGCTG GATCCCCCAG GCAGCGTCTC AAGATCACAA CAGTCGCAGA TATATACGAG TCCATGCGTG GCCATCAAGG ACCCAAATTA AAATT'CTAAT ACGGAAAAGA AGGATAAACA GATTACTCAG TATTTTTGCA CAAAGCAAGT
CTAAGCGTCT GAGCTGATCG CTGGACATTG AAACACGACT AAAAGAAGTG ATACCTCGTA GAGGTGAAAC ATACACCACA AAAGAAGTTC TCAGATTATC AAAAAACTAA GTATCCAATT CCATCAAATC GATTCCTTTT A
GCT'GCTCTTA AGAGGCTGGC ATTCCGTAGG TCCATCATAA TTGAAGAAGC GCCAAATIGA
GATGCACGGG GGAGT'GTCAA TTGTGCGGTT GAGAAGAAGA AGCCAACTTA TTGAAGT'GGA TGITTTATGG AGGTCATATT'
Nucleotide sequence of a cDNA clone of Xenopus platelet-derived growth factor A-chain Bruce E.Bejcek, Dean Y.Li and Thomas F.Deuel* Jewish Hospital of St Louis, 216 S. Kingshighway, St Louis, MO 63110, USA Submitted December 12, 1989
The nucleotide sequence of a cDNA clone homologous to the A-chain of the human platelet derived growth factor and isolated from a Xenopus oocyte cDNA library (1) is presented. The leader peptide is coded for by nucleotides 14-79, prepro sequences are coded for by nucleotides 80-286 and the mature protein is coded for by nucleotides 287-658. This clone is homologous
1 GCAGCAGGAC GCAATGAGGA TCTGGGCTTG 61 CCTGTCTCCT TCATTGGGCG AGGAAGCAGA 121 TCACAGCGAA ATCCGCAGCA TCAGCGACCT 181 AGGAGGCGAG GATGCTTCTG CAGCCAACAT 241 TAGGCTGGTG CCAGAAAAGC GCTCCGTTCC 301 AGTCCCTGCT ATCTGTAAAA CAAGAACTGT 361 TCCAACATCT GCCAATTTTC TGATCTGGCC 421 ATGCTGCAAC ACCAGCAGTG TCAAGTGCCA 481 GGTGGCAAAA GTAGAATATG TAAGAAAGAA 541 AGAGGAACAT CTGGAATGCA CGTGTACAGC 601 AACTGGAAGG ACTAGGGAAA CAGGTAAAAA 661 ATAACCGTCT GGAAAGTAAT TTTGAACGGC 721 AATGGAAAAA AGGCATTGAG CAGATCCAAA 781 TTTATGGTTT ATGGTTTATT TTCATGATAT 841 TTTGGTCAGC TGGAAAATCA AATTCTGTTG
*
To whom correspondence should be addressed
EMBL accession no. X17545
to the long form previously described (2) but it is missing a 33 nucleotide insertion beginning at nucleotide 604.
REFERENCES 1. Rabagliati,M.R. and Melton,D.A. (1987) Cell 48, 599-605. 2. Mercola,M., Melton,D.A. and Stiles,C.D. (1988) Science 243, 800-804.
GATTCTGCTG GATCCCCCAG GCAGCGTCTC AAGATCACAA CAGTCGCAGA TATATACGAG TCCATGCGTG GCCATCAAGG ACCCAAATTA AAATT'CTAAT ACGGAAAAGA AGGATAAACA GATTACTCAG TATTTTTGCA CAAAGCAAGT
CTAAGCGTCT GAGCTGATCG CTGGACATTG AAACACGACT AAAAGAAGTG ATACCTCGTA GAGGTGAAAC ATACACCACA AAAGAAGTTC TCAGATTATC AAAAAACTAA GTATCCAATT CCATCAAATC GATTCCTTTT A
GCT'GCTCTTA AGAGGCTGGC ATTCCGTAGG TCCATCATAA TTGAAGAAGC GCCAAATIGA
GATGCACGGG GGAGT'GTCAA TTGTGCGGTT GAGAAGAAGA AGCCAACTTA TTGAAGT'GGA TGITTTATGG AGGTCATATT'
