_ GtnCAr
1056 Nucleic Acids Research, Vol. 18, No. 4
Nucleotide oxidase III
sequence
.::) Oxford Universiiy Press 1990
of carp mitochondrial cytochrome
C
Chang Jen Huang1'2, Fore Lien Huang 1,3, Yea Sha Chang1'2, Yuan Jang Tsai3 and Tung Bin Lo ,2,* 'Institute of Biological Chemistry, Academia Sinica, 2Graduate Institute of Biochemical Sciences and 3Department of Zoology, National Taiwan University, Taipei, Taiwan EMBL accession no. Xl 7006
Submitted January 11, 1990
We have reported several cDNA clones encoding a and (3 subunits of carp gonadotropin from a pituitary gland cDNA library (1). However, there is a weak positive cDNA clone, pc 1258, whose DNA and deduced amino acid sequences shown below have high degree of homology with the sequences of mitochrondrial cytochrome C oxidase subunit Ill found in many species (2-7). The percentage of homology of DNA and amino acid sequence is 76 and 81.2, respectively, as compared to the human molecule; 75.4 and 81.2 to bovine; 77.4 and 80.0 to mouse; 79.5 and 90.0 to Xenopus; 64.6 and 65.5 to Drosophila; 55.2 and 45 to yeast. The first 29 bp, underlined in the figure, was absent in pc 1258 and these sequences were obtained by a synthetic primer, complementary and reverse to the sequence from bpl30 to bpl55 underlined in the figure, in a subclone of
4.3 kb EcoRI fragment of carp mitochondrial DNA. Determination of the rest of the whole mitochondrial DNA sequence is currently being undertaken.
REFERENCES
1. Chang,Y.S., Huang,C.J., Huang,F.L. and Lo,T.B. (1988) Int. J. Peptide Protein Res. 32, 556-564. 2. Anderson,S. et al. (1981) Nature 290, 457-465. 3. Anderson,S. et al. (1982) J. Mol. Biol. 156, 683-717. 4. Bibb,M.J. et al. (1981) Cell 26, 167-180. 5. Clary,D.O. et al. (1982) Nucl. Acids Res. 10, 6619-6637. 6. Roe,B.A., Ma,D.-P., Wilson,R.K. and Wong,J.F.-H. (1985) J. Biol. Chem. 260, 9759-9774. 7. Thalenfeld,B.E. and Tzagoloff,A. (1980) J. Biol. Chem. 255, 6174-6180.
75
M A H Q A H A Y H M V D P S P W P L T G A I A A L
~
L I T S G L A I W F H F H S T T L I T L G L I lI C L L T M Y Q W W R D I I R E G T F Q G H H T P CAMAA Q K G L R Y G M I L F I T S E V F F F L G F F _ TICTACCAGAA=AX3GAX3GA: Iu F Y H S S L A P T P E L G G C W P P T G M T P _ A g =AACC E A P F E V P L L N T A V L L A S G V T V T W A H >_k > ~ ____ *A I M E G D R K Q A I Q S L A L T I L L G L Y F
7150
L L 225
P V
300
W A
375
L D H S ~ T A
450 525 ~~~~~~~~~~~~
600
L Q A M E Y Y E A P F T I A D G V Y G S T F F V A IC:AATAC 675 T G F H G L H V I I G S T F L A V C L L R Q I Q Y CAC[TIACA]CACA35I(IIt;zmAAC:ZA.lG 750 H F T S E H H F G F E A A A W Y W H F V D V V W L TCIACGA[CAA[ L. 809 F L Y V S I Y W W G S
*
To whom correspondence should be addressed
1056 Nucleic Acids Research, Vol. 18, No. 4
Nucleotide oxidase III
sequence
.::) Oxford Universiiy Press 1990
of carp mitochondrial cytochrome
C
Chang Jen Huang1'2, Fore Lien Huang 1,3, Yea Sha Chang1'2, Yuan Jang Tsai3 and Tung Bin Lo ,2,* 'Institute of Biological Chemistry, Academia Sinica, 2Graduate Institute of Biochemical Sciences and 3Department of Zoology, National Taiwan University, Taipei, Taiwan EMBL accession no. Xl 7006
Submitted January 11, 1990
We have reported several cDNA clones encoding a and (3 subunits of carp gonadotropin from a pituitary gland cDNA library (1). However, there is a weak positive cDNA clone, pc 1258, whose DNA and deduced amino acid sequences shown below have high degree of homology with the sequences of mitochrondrial cytochrome C oxidase subunit Ill found in many species (2-7). The percentage of homology of DNA and amino acid sequence is 76 and 81.2, respectively, as compared to the human molecule; 75.4 and 81.2 to bovine; 77.4 and 80.0 to mouse; 79.5 and 90.0 to Xenopus; 64.6 and 65.5 to Drosophila; 55.2 and 45 to yeast. The first 29 bp, underlined in the figure, was absent in pc 1258 and these sequences were obtained by a synthetic primer, complementary and reverse to the sequence from bpl30 to bpl55 underlined in the figure, in a subclone of
4.3 kb EcoRI fragment of carp mitochondrial DNA. Determination of the rest of the whole mitochondrial DNA sequence is currently being undertaken.
REFERENCES
1. Chang,Y.S., Huang,C.J., Huang,F.L. and Lo,T.B. (1988) Int. J. Peptide Protein Res. 32, 556-564. 2. Anderson,S. et al. (1981) Nature 290, 457-465. 3. Anderson,S. et al. (1982) J. Mol. Biol. 156, 683-717. 4. Bibb,M.J. et al. (1981) Cell 26, 167-180. 5. Clary,D.O. et al. (1982) Nucl. Acids Res. 10, 6619-6637. 6. Roe,B.A., Ma,D.-P., Wilson,R.K. and Wong,J.F.-H. (1985) J. Biol. Chem. 260, 9759-9774. 7. Thalenfeld,B.E. and Tzagoloff,A. (1980) J. Biol. Chem. 255, 6174-6180.
75
M A H Q A H A Y H M V D P S P W P L T G A I A A L
~
L I T S G L A I W F H F H S T T L I T L G L I lI C L L T M Y Q W W R D I I R E G T F Q G H H T P CAMAA Q K G L R Y G M I L F I T S E V F F F L G F F _ TICTACCAGAA=AX3GAX3GA: Iu F Y H S S L A P T P E L G G C W P P T G M T P _ A g =AACC E A P F E V P L L N T A V L L A S G V T V T W A H >_k > ~ ____ *A I M E G D R K Q A I Q S L A L T I L L G L Y F
7150
L L 225
P V
300
W A
375
L D H S ~ T A
450 525 ~~~~~~~~~~~~
600
L Q A M E Y Y E A P F T I A D G V Y G S T F F V A IC:AATAC 675 T G F H G L H V I I G S T F L A V C L L R Q I Q Y CAC[TIACA]CACA35I(IIt;zmAAC:ZA.lG 750 H F T S E H H F G F E A A A W Y W H F V D V V W L TCIACGA[CAA[ L. 809 F L Y V S I Y W W G S
*
To whom correspondence should be addressed
