5898 Nucleic Acids Research, Vol. 18, No. 19
Nucleotide sequence of rice waxy gene Zong-yang Wang, Zhi-liang Wu, Yan-yan Xing, Fei-gin Zheng, Xiao-Ii Guo, Wei-guo Zhang and Meng-min Hong Shanghai Institute of Plant Physiology, Academia Sinica, 300 Fenglin Road, Shanghai 200032, China Submitted on August 9, 1990
Rice waxy gene codes for the starch granule-bound starch synthase (EC 2.4.1.11) (1). To characterize its molecular structure, a rice (Oryza sativa subsp. japonica Hengfeng cultivar) genomic library was constructed in XEMBL-3 vector. Two overlapping genomic clones containing waxy gene sequence were screened and identified from this library by using maize waxy gene DNA as probe (kindly provided by Dr N.Fedoroff). The nucleotide sequences of rice waxy gene and its 5' and 3' flanking regions (5499 bp) are presented here. Alignment of nucleotide sequence of waxy gene from rice with that of maize (2) and barley (3) revealed the presence of 13 introns and 14 exons. The fulllength of waxy preprotein is 609 amino acid residues.
EMBL
accession no.
X53694
ACKNOWLEDGEMENTS We are deeply grateful to Dr Wu Mm for her helpful discussion. This study was supported by grants from the High Technology Development Programme of China, and the Rockefeller Foundation.
REFERENCES 1.
Preiss,J. (1988) 7he Biochemistry of Plants, Academic Press, Vol. 14, pp. 181-254.
2. Klosgen,R.B., Gierl,A., Schwarz-Sommer,Z. and Saedler,H. (1986) Mol. Gen. Genet. 203, 237-244.
3. Rohde,W., Becker,D. and Salamini,F. (1988) Nucl. Acids Res. 16, 7185-7186.
CAGCAGAGGAAAAATGGCGAGGGAGAAAGGTGTTGCCCGAAAATGCCACGGCGAGGTATCTGCTTCTGTGTGTGTGTNATACTGTGATGTGATCGATCACATGCATCTTTCATTGCTCGTTTTTCCTTACAAGTAGTC TCATACATGCTAATTTCTGTAAGGTGTTGGGCTGGAAATTAATTAATTAATTAATTGACTTGCCAAGATCCATATATATGTCCTGATATTAAATCTTCGTTCGTTATGTTTGGTTAGGCTGATCAATGTTATTCTAGAGT CTAGAGAAACACACCCAGGGGTTTTCCAACTAGCTCCACAAGATGGTGGGCTAGCTGACCTAGATTTGAAGTCTCACTCCTTATAATTATTTTATATTAGATCATTTTCTAATATTCGTGTCTTTTTTTATTCTAGAGTC TAGATCTTGTGTTCAACTCTCGTTAAATCATGTCTCTCGCCACTGGAGAAACAGATCAGGAGGGTTTATTTTGGGTATAGGTCAAAGCTAAGATTGAAATTCACAAATAGTAAAATCAGAATCCAACCAATTTTAGTAGC CGAGTTGGTCAAAGGAAAATGTATATAGCTAGATTTATTGTTTTGGCAAAAAAAAATCTGAATATGCAAAATACTTGTATATCTTTGTATTAAGAAGATGAAAATAAGTAGCAGAAAATTAAAAAATGGATTATATTTCC TGGGCTAAAAGAATTGTTGATTTGGCACAATTAAATTCAGTGTCAAGGTTTTGTGCAAGAATTCAGTGTGAAGGAATAGATTCTCTTCAAAACAATTTAATCATTCATCTGATCTGCTCAAAGCTCTGTGCATCTCCGGG
TGCAACGGCCAGGATATTTATTGTGCAGTAAAAAAATGTCATATCCCCTAGCCACCCAAGAAACTGCTCCTTAAGTCCTTATAAGCACATATGGCATTGTAATATATATGTTTGAGTTTTAGCGACAATTTTTTTAAAAA CTTTTGGTCCTTTTTATGAACGTTTTAAGTTTCACTGTCTTTTTTTTTCGAATTTTAAATGTA-GCTTCAAATTCTAATCCCCAATCCAAATTGTAATAAACTTCAATTCTCCTAATTAACATCTTAATTCATTTATTTGA AAACCAGTTCAAATTCTTTTAGGCTCACCAAACCTTAAACAATTCAATTCAGTGCAGAGATCTTCCACAGAACAGCTAGACAACCACCATGTCGGCTCTCACCACGTCCCAGCTCGCCACCTCGGCCACCGGCTTCGGCA TCGCCGACAGGTCGGCGCCGTCGTCGCTGCTCCGCCACGGGTTCCAGGGCCTCAAGCCCCGCAGCCCCGCCGGCGGCGACGCGACGTCGCTCAGCGTGACGACCAGCGCGCGCGCGACGCCCAAGCAGCAGCGGTCGGTG CAGCGTGGCAGCCGGAGGTTCCCCTCCGTCGTCGTGTACGCCACCGGCGCCGGCATGAACGTCGTGTTCGTCGGCGCCGAGATGGCCCCCTGGAGCAAGACCGGCGGCCTCGGTGACGTCCTCGGTGGCCTCCCCCCTGC CATGGCTg taagcacacacaaac ttcgatcgctcgtcgtcgctgaccg tcgtcgtct tcaac tg ttc ttgatcagtgcat tggatggatg tgtaatgttgtgt tc ttgtgt tc t ttgcagGCGAATGGCCACAGGGTCAT GGTGATCTCTCCTCGGTACGACCAGTACAAGGACGCTTGGGATACCAGCGTTGTGGCTGAGg taggagc atat gcg tgat cagat cat cac aagat cgat tagct t tagat ga tt tg t taca tt t cgcaaga tt t taac c caagt tt tgtggtgcaat tcattgcagATCAAGGTTGCAGACAGGTACGAGAGGGTGAGGTTTTTCCATTGCTACAAGCGTGGAGTCGACCGTGTGTTCATCGACCATCCGTCATTCCTGGAGAAGg tggagtcatcat
tag t ttacct tt t ttg tt t ttac tgaat tat taacag tgcat ttagcag ttggac tgagc ttagc ttccac tgg9tgat ttcagGTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATTACAAA GACAACCAGATGCGTTTCAGCCTTCTTTGCCAGgtcagtgat tac ttc tatc tgatgatgg ttggaagcatcacgagt ttaccatagtatgtatggat tcataac taat tcgtg tat tgatgc tacc tgcagGCAGCACT CGAGGCTCCTAGGATCCTAAACCTCAACAACAACCCATACTTCAAAGGAACTTATGgtgagt tacaat tgatctcaagatc ttataact ttct tcgaaggaatccatgatgatcagactaat tcc ttccggt ttgt tac t tc tatgc tatg t tc t tgccataccaac tcaa gacaacagGTGAGGATGTTGTGTTCGTCTGCAACGACTGGCACACTGGCCCACTGGCGAGCTACCTGAAGAACAACTACCAGCCCAATGGCATCTACAGGAATGCAAAGg at c tgcatgc acac tgc att ct gt t cagaaac tgac t gtc tgaatc tt tt t cac tgcagGTTGCTTTCTGCATCCACAACATCTCCTACCAGGGCCGTTTCGCTTTCGAGGATTACCCTGAGCTGAACCTCTCCGAGAGG TTCAGGTCATCCTTCGATTTCATCGACGGgtatgagtaagat tc taagagtaac ttac tg tcaat tcgccatatatcgat tcaatccaagatcct t ttgagc tgacaaccc tgcac tac tgtccatcgt tcaaatccggt
t aaa tt t cagGTATGACACGCCGGTGGAGGGCAGGAAGATCAACTGGATGAAGGCCGGAATCCTGGAAGCCGACAGGGTGCTCACCGTGAGCCCGTACTACGCCGAGGAGCTCATCTCCGGCATCGCCAGGGGATGCGAG CTCGACAACATCATGCGGCTCACCGGCATCACCGGCATCGTCAACGGCATGGACGTCAGCGAGTGGGATCCTAGCAAGGACAAGTACATCACCGCCAAGTACGACGCAACCACGg taag aac gaat gca tt ct t cac aag atatgcaatctgaattttctttgaaaaagaaattatcatctgtcacttcttgattgattctgacaaggcaagaatgagtgacaaatttcagGCAATCGAGGCGAAGGCGCTGAACAAGGAGGCGTTGCAGGCGGAGGCGG GTCTCCCGGTCGACAGGAAAATCCCACTGATCGCGTTCATCGGCAGGCTGGAGGAACAGAAGGGCCCTGACGTCATGGCCGCCGCCATCCCGGAGCTCATGCAGGAGGACGTCCAGATCGTTCTTCTGgtataatataat acactacaagacacact tgcacgatatgccaaaaat tcagaacaaat tcagtggcaaaaaaaaaac tcgaatat taataatatcaaataat
tagggaaggacc
tagaaggggtgaggc t ttgaacccagatcgtctagtc
caccacc ttgtggag ttagccggaacacc tc tgagcat ttctcaat tcagtggcaaatgatg tg tataat t ttgatccgtgtg tgt ttcagGGTACTGGAAAGAAGAAGTTCGAGAAGCTGCTCAAGAGCATGGAGGAGA
AGTAT CCGGGCAAGGTGAGGGC CGTGGTGAAGTTCAACGCGCCGCTTGCT CAT CT CAT CATGG CCGGAGC CGACGTGCT CGCCGTC C CCAGCCGCTT CGAGCC CTGTGGACT CAT CCAGCTGCAGGGGATGAGATACGGA ACGgaatacaatt tccatc tatcaattcgattgt tcgat ttcatc tt tgtgcaatgcaatgcaat tgcaaatgcaaatgcatgatgat t ttcc ttgt tgat ttctccagCCCTGTGCTTGCGCGTCCACCGGTGGGCTCG TGGACACGGTCATCGAAGGCAAGACTGGTTTCCACATGGGCCGTCTCAGCGTCGACg taagcc tatacat ttacataacaatcagatat gacacatcc taataccgataag tcgg tacac tac tacacat ttacatggt t gc tgg9tt a tatg9g9t tt tt t tggcagTGCAAGGTGGTGGAGCCAAGCGACGTGAAGAAGGTGGCGGCCACCCTGAAGCGCGCCATCAAGGTCGTCGGCACGCCGGCGTACGAGGAGATGGTCAGGAACTGCATGAACCAGG ACCTCTCCTGGAAGgtataaat tacgaaacaaat ttaacccaaacatatac tatatac tccc tccgc ttc taaatat tcaacgccg ttgtc t ttt ttaaatatat ttgaccat tcgtc ttat taaaaaaat taaataat t ataaattctt ttcctatcat ttgat tcattgttaaatatac ttatatgtatacatatagt t ttacatat ttcataaaat tt t ttgaacaagacgaacggtcaaacatgtgc taaaaagt taacggtgtcgaatat tcaga aacggagggagtataaacg tc ttgt tcagaag ttcagagat tcacc tg tctgatgc tgatgatgat taat tg t ttgcaacatggat ttcagGGGCCTGCGAAGAACTGGGAGAATGTGCTCCTGGGCCTGGGCGTCGCCG
GCAGCGCGCCGGGGATCGAAGGCGACGAGATCGCGCCGCTCGCCAAGGAGAACGTGGCTGCTCCTTGAAGAGCCTGAGATCTACATATGGAGTGATTAATTAATATAGCAGTATATGGATGAGAGACGAATGAACCAGTG GTTTGTTTGTTGTAGTGAATTTGTAGCTATAGCCAATTATATAGGCTAATAAGTTTGATGTTGTACTCTTCTGGGTGTGCTTAAGTATCTTATCGGACCCTGAATTTATGTGTGTGGCTTATTGCCAATAATATTAAGTA ATAAAGGGTTTATTATATTATTATATATGTTATATTATACTTCCCCTGTTCCATATTATACCATGCCATTTTTGTTTTATGCCAAGTCAAACTTTTTATATTTAACCAAATTTATAAAAATAAATATAGCAACATTTGTA ATACTGAACTATTTTTTTGTTAGACAGACTGTCAAAACTTAAATTATAGGTACTATATTTGTCTCAAAATATAATAACTTTTAGTTATGTATCTGGGTATGTGTCTGTCTATATGTGTAGCT-AAAAGTTGTTTTGTGTCA AAAAAAATGTTATTATATTTTTTTTATAAATTTATTTAAGTTTGAAGGAGCAGTAGTTTGACTCAGGATAAGATGTAAAATAATTTATAATATACT CT CTCGT CCCATTTTAAATGCAACCAAAACTTTGAT CGTTTAT C TTATTTATTTTTTTATAATTAATACTTTTATTGTTATGAGATAATAAAACATGAATAGTACTTTATACATGACTTATGTTTTTAATTTTTTTAAATAAAACGAATGATTAAAATTATGCACGAAAAATTATAGTTGCACT TAAAATGTGACGGAGGGAGTGGATACGAAGGAACTAGGT
Figure. Complete nucleotide sequence of rice Wx gene. The intervening sequences are given in small letters. The translation start and stop codons, potential polyadenylation signals as well as putative TATA box, Agga box and enhancer-like sequence are indicated by underline.
Nucleotide sequence of rice waxy gene Zong-yang Wang, Zhi-liang Wu, Yan-yan Xing, Fei-gin Zheng, Xiao-Ii Guo, Wei-guo Zhang and Meng-min Hong Shanghai Institute of Plant Physiology, Academia Sinica, 300 Fenglin Road, Shanghai 200032, China Submitted on August 9, 1990
Rice waxy gene codes for the starch granule-bound starch synthase (EC 2.4.1.11) (1). To characterize its molecular structure, a rice (Oryza sativa subsp. japonica Hengfeng cultivar) genomic library was constructed in XEMBL-3 vector. Two overlapping genomic clones containing waxy gene sequence were screened and identified from this library by using maize waxy gene DNA as probe (kindly provided by Dr N.Fedoroff). The nucleotide sequences of rice waxy gene and its 5' and 3' flanking regions (5499 bp) are presented here. Alignment of nucleotide sequence of waxy gene from rice with that of maize (2) and barley (3) revealed the presence of 13 introns and 14 exons. The fulllength of waxy preprotein is 609 amino acid residues.
EMBL
accession no.
X53694
ACKNOWLEDGEMENTS We are deeply grateful to Dr Wu Mm for her helpful discussion. This study was supported by grants from the High Technology Development Programme of China, and the Rockefeller Foundation.
REFERENCES 1.
Preiss,J. (1988) 7he Biochemistry of Plants, Academic Press, Vol. 14, pp. 181-254.
2. Klosgen,R.B., Gierl,A., Schwarz-Sommer,Z. and Saedler,H. (1986) Mol. Gen. Genet. 203, 237-244.
3. Rohde,W., Becker,D. and Salamini,F. (1988) Nucl. Acids Res. 16, 7185-7186.
CAGCAGAGGAAAAATGGCGAGGGAGAAAGGTGTTGCCCGAAAATGCCACGGCGAGGTATCTGCTTCTGTGTGTGTGTNATACTGTGATGTGATCGATCACATGCATCTTTCATTGCTCGTTTTTCCTTACAAGTAGTC TCATACATGCTAATTTCTGTAAGGTGTTGGGCTGGAAATTAATTAATTAATTAATTGACTTGCCAAGATCCATATATATGTCCTGATATTAAATCTTCGTTCGTTATGTTTGGTTAGGCTGATCAATGTTATTCTAGAGT CTAGAGAAACACACCCAGGGGTTTTCCAACTAGCTCCACAAGATGGTGGGCTAGCTGACCTAGATTTGAAGTCTCACTCCTTATAATTATTTTATATTAGATCATTTTCTAATATTCGTGTCTTTTTTTATTCTAGAGTC TAGATCTTGTGTTCAACTCTCGTTAAATCATGTCTCTCGCCACTGGAGAAACAGATCAGGAGGGTTTATTTTGGGTATAGGTCAAAGCTAAGATTGAAATTCACAAATAGTAAAATCAGAATCCAACCAATTTTAGTAGC CGAGTTGGTCAAAGGAAAATGTATATAGCTAGATTTATTGTTTTGGCAAAAAAAAATCTGAATATGCAAAATACTTGTATATCTTTGTATTAAGAAGATGAAAATAAGTAGCAGAAAATTAAAAAATGGATTATATTTCC TGGGCTAAAAGAATTGTTGATTTGGCACAATTAAATTCAGTGTCAAGGTTTTGTGCAAGAATTCAGTGTGAAGGAATAGATTCTCTTCAAAACAATTTAATCATTCATCTGATCTGCTCAAAGCTCTGTGCATCTCCGGG
TGCAACGGCCAGGATATTTATTGTGCAGTAAAAAAATGTCATATCCCCTAGCCACCCAAGAAACTGCTCCTTAAGTCCTTATAAGCACATATGGCATTGTAATATATATGTTTGAGTTTTAGCGACAATTTTTTTAAAAA CTTTTGGTCCTTTTTATGAACGTTTTAAGTTTCACTGTCTTTTTTTTTCGAATTTTAAATGTA-GCTTCAAATTCTAATCCCCAATCCAAATTGTAATAAACTTCAATTCTCCTAATTAACATCTTAATTCATTTATTTGA AAACCAGTTCAAATTCTTTTAGGCTCACCAAACCTTAAACAATTCAATTCAGTGCAGAGATCTTCCACAGAACAGCTAGACAACCACCATGTCGGCTCTCACCACGTCCCAGCTCGCCACCTCGGCCACCGGCTTCGGCA TCGCCGACAGGTCGGCGCCGTCGTCGCTGCTCCGCCACGGGTTCCAGGGCCTCAAGCCCCGCAGCCCCGCCGGCGGCGACGCGACGTCGCTCAGCGTGACGACCAGCGCGCGCGCGACGCCCAAGCAGCAGCGGTCGGTG CAGCGTGGCAGCCGGAGGTTCCCCTCCGTCGTCGTGTACGCCACCGGCGCCGGCATGAACGTCGTGTTCGTCGGCGCCGAGATGGCCCCCTGGAGCAAGACCGGCGGCCTCGGTGACGTCCTCGGTGGCCTCCCCCCTGC CATGGCTg taagcacacacaaac ttcgatcgctcgtcgtcgctgaccg tcgtcgtct tcaac tg ttc ttgatcagtgcat tggatggatg tgtaatgttgtgt tc ttgtgt tc t ttgcagGCGAATGGCCACAGGGTCAT GGTGATCTCTCCTCGGTACGACCAGTACAAGGACGCTTGGGATACCAGCGTTGTGGCTGAGg taggagc atat gcg tgat cagat cat cac aagat cgat tagct t tagat ga tt tg t taca tt t cgcaaga tt t taac c caagt tt tgtggtgcaat tcattgcagATCAAGGTTGCAGACAGGTACGAGAGGGTGAGGTTTTTCCATTGCTACAAGCGTGGAGTCGACCGTGTGTTCATCGACCATCCGTCATTCCTGGAGAAGg tggagtcatcat
tag t ttacct tt t ttg tt t ttac tgaat tat taacag tgcat ttagcag ttggac tgagc ttagc ttccac tgg9tgat ttcagGTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATTACAAA GACAACCAGATGCGTTTCAGCCTTCTTTGCCAGgtcagtgat tac ttc tatc tgatgatgg ttggaagcatcacgagt ttaccatagtatgtatggat tcataac taat tcgtg tat tgatgc tacc tgcagGCAGCACT CGAGGCTCCTAGGATCCTAAACCTCAACAACAACCCATACTTCAAAGGAACTTATGgtgagt tacaat tgatctcaagatc ttataact ttct tcgaaggaatccatgatgatcagactaat tcc ttccggt ttgt tac t tc tatgc tatg t tc t tgccataccaac tcaa gacaacagGTGAGGATGTTGTGTTCGTCTGCAACGACTGGCACACTGGCCCACTGGCGAGCTACCTGAAGAACAACTACCAGCCCAATGGCATCTACAGGAATGCAAAGg at c tgcatgc acac tgc att ct gt t cagaaac tgac t gtc tgaatc tt tt t cac tgcagGTTGCTTTCTGCATCCACAACATCTCCTACCAGGGCCGTTTCGCTTTCGAGGATTACCCTGAGCTGAACCTCTCCGAGAGG TTCAGGTCATCCTTCGATTTCATCGACGGgtatgagtaagat tc taagagtaac ttac tg tcaat tcgccatatatcgat tcaatccaagatcct t ttgagc tgacaaccc tgcac tac tgtccatcgt tcaaatccggt
t aaa tt t cagGTATGACACGCCGGTGGAGGGCAGGAAGATCAACTGGATGAAGGCCGGAATCCTGGAAGCCGACAGGGTGCTCACCGTGAGCCCGTACTACGCCGAGGAGCTCATCTCCGGCATCGCCAGGGGATGCGAG CTCGACAACATCATGCGGCTCACCGGCATCACCGGCATCGTCAACGGCATGGACGTCAGCGAGTGGGATCCTAGCAAGGACAAGTACATCACCGCCAAGTACGACGCAACCACGg taag aac gaat gca tt ct t cac aag atatgcaatctgaattttctttgaaaaagaaattatcatctgtcacttcttgattgattctgacaaggcaagaatgagtgacaaatttcagGCAATCGAGGCGAAGGCGCTGAACAAGGAGGCGTTGCAGGCGGAGGCGG GTCTCCCGGTCGACAGGAAAATCCCACTGATCGCGTTCATCGGCAGGCTGGAGGAACAGAAGGGCCCTGACGTCATGGCCGCCGCCATCCCGGAGCTCATGCAGGAGGACGTCCAGATCGTTCTTCTGgtataatataat acactacaagacacact tgcacgatatgccaaaaat tcagaacaaat tcagtggcaaaaaaaaaac tcgaatat taataatatcaaataat
tagggaaggacc
tagaaggggtgaggc t ttgaacccagatcgtctagtc
caccacc ttgtggag ttagccggaacacc tc tgagcat ttctcaat tcagtggcaaatgatg tg tataat t ttgatccgtgtg tgt ttcagGGTACTGGAAAGAAGAAGTTCGAGAAGCTGCTCAAGAGCATGGAGGAGA
AGTAT CCGGGCAAGGTGAGGGC CGTGGTGAAGTTCAACGCGCCGCTTGCT CAT CT CAT CATGG CCGGAGC CGACGTGCT CGCCGTC C CCAGCCGCTT CGAGCC CTGTGGACT CAT CCAGCTGCAGGGGATGAGATACGGA ACGgaatacaatt tccatc tatcaattcgattgt tcgat ttcatc tt tgtgcaatgcaatgcaat tgcaaatgcaaatgcatgatgat t ttcc ttgt tgat ttctccagCCCTGTGCTTGCGCGTCCACCGGTGGGCTCG TGGACACGGTCATCGAAGGCAAGACTGGTTTCCACATGGGCCGTCTCAGCGTCGACg taagcc tatacat ttacataacaatcagatat gacacatcc taataccgataag tcgg tacac tac tacacat ttacatggt t gc tgg9tt a tatg9g9t tt tt t tggcagTGCAAGGTGGTGGAGCCAAGCGACGTGAAGAAGGTGGCGGCCACCCTGAAGCGCGCCATCAAGGTCGTCGGCACGCCGGCGTACGAGGAGATGGTCAGGAACTGCATGAACCAGG ACCTCTCCTGGAAGgtataaat tacgaaacaaat ttaacccaaacatatac tatatac tccc tccgc ttc taaatat tcaacgccg ttgtc t ttt ttaaatatat ttgaccat tcgtc ttat taaaaaaat taaataat t ataaattctt ttcctatcat ttgat tcattgttaaatatac ttatatgtatacatatagt t ttacatat ttcataaaat tt t ttgaacaagacgaacggtcaaacatgtgc taaaaagt taacggtgtcgaatat tcaga aacggagggagtataaacg tc ttgt tcagaag ttcagagat tcacc tg tctgatgc tgatgatgat taat tg t ttgcaacatggat ttcagGGGCCTGCGAAGAACTGGGAGAATGTGCTCCTGGGCCTGGGCGTCGCCG
GCAGCGCGCCGGGGATCGAAGGCGACGAGATCGCGCCGCTCGCCAAGGAGAACGTGGCTGCTCCTTGAAGAGCCTGAGATCTACATATGGAGTGATTAATTAATATAGCAGTATATGGATGAGAGACGAATGAACCAGTG GTTTGTTTGTTGTAGTGAATTTGTAGCTATAGCCAATTATATAGGCTAATAAGTTTGATGTTGTACTCTTCTGGGTGTGCTTAAGTATCTTATCGGACCCTGAATTTATGTGTGTGGCTTATTGCCAATAATATTAAGTA ATAAAGGGTTTATTATATTATTATATATGTTATATTATACTTCCCCTGTTCCATATTATACCATGCCATTTTTGTTTTATGCCAAGTCAAACTTTTTATATTTAACCAAATTTATAAAAATAAATATAGCAACATTTGTA ATACTGAACTATTTTTTTGTTAGACAGACTGTCAAAACTTAAATTATAGGTACTATATTTGTCTCAAAATATAATAACTTTTAGTTATGTATCTGGGTATGTGTCTGTCTATATGTGTAGCT-AAAAGTTGTTTTGTGTCA AAAAAAATGTTATTATATTTTTTTTATAAATTTATTTAAGTTTGAAGGAGCAGTAGTTTGACTCAGGATAAGATGTAAAATAATTTATAATATACT CT CTCGT CCCATTTTAAATGCAACCAAAACTTTGAT CGTTTAT C TTATTTATTTTTTTATAATTAATACTTTTATTGTTATGAGATAATAAAACATGAATAGTACTTTATACATGACTTATGTTTTTAATTTTTTTAAATAAAACGAATGATTAAAATTATGCACGAAAAATTATAGTTGCACT TAAAATGTGACGGAGGGAGTGGATACGAAGGAACTAGGT
Figure. Complete nucleotide sequence of rice Wx gene. The intervening sequences are given in small letters. The translation start and stop codons, potential polyadenylation signals as well as putative TATA box, Agga box and enhancer-like sequence are indicated by underline.
