6420
Nucleic Acids Research, Vol. 18, No. 21
Nucleotide sequence of the Adh lebanonensis
gene
of Drosophila
E.Juan, M.Papaceit and A.Quintana Departament de Genetica, Universitat de Barcelona, 08071 Barcelona, Spain Submitted October 3, 1990
D. lebanonensis belongs to the victoria group of the subgenus Scaptodrosophila. The Adh gene was isolated from a partial BSKS+ plasmid library constructed with the 12Kb XbaI genomic fraction, the fragment XbaI-EcoRI was subcloned in BSKS (+) and BSKS (-) and a series of 14 or 12 nested deletions (obtained by the Exo-Mung method) for the non-coding and coding strand respectively were single strand sequenced with T7 DNA polymerase (Sequenase). The two introns in the coding region were unambiguously identified since the protein has been sequenced (Villarroya et al., 1989). Three putative TATA boxes have been identified, one of them with a change in the sequence,
1 101 201 301 401 501 601 701 801 901 1001 1101 1201 1301 1401 1501 1601 1701 1801 1901 2001 2101 2201
2301 2401
2501 2601 2701
2801 2901 3001
EMBL accession
no.
X53429
compared to the Drosophila species with only one Adh gene which have two promoters. To assign the initiation start sites correctly mRNA analysis is in progress. ACKNOWLEDGEMENT Supported by DGICYT PB87-0136 REFERENCE 1. Vilarroya,A., Juan,E., Egestad,B. and Jomvall,H. (1989) Eur. J. Biochem. 180, 191-197.
tcgaggtcctagaaacacactcagtgcsccctagttgagaaactgtactcgtaagtcaagtgctgacaataatctacacacatctacttgtacatattta caaatacgttgcatcagcgactctcagjagcacatgctcgtattacatatttacatacgagtatgtactaagtacatatatagtacatagtatgcgaacac acagcactatacaagtataatgcaaactacatttatacttgt.atgtaaatgagaaatctactcttttcttggcattgagcactggcgtccaatctgttgg cacagagctctgctggcatcaaataccggtcattcccattcctaggcatgttacaattattggatttgcatacctccgctctattgccatataagcttag tttttgtgctcagccttgagtggttcgctattaaaaaggcagctgctcacatcccctggatgactggggtcggctctggtacaatgtgcacctactaggg gcctgtttgtgagctcggcgcacccccaatcttttaaattgaaggtgatcttctggtcgctctgttgctgccctgcgcttatttctttgacattcactga aggtgaaaacagcatgtgccgggcccggcgggcccggacccgagcgccggtcgcttgtttacatatagttcatacacatacgatacatacgagtatgtac atacgtgggcaattgtataagcattattacaaaatatagaatgcaaaatacacacgagtcgtcgggttggcgatgctttcagctcttccgcgttcatctg gctgggtatttaaagcgcgttataccctaggacgacgattattgtcagtgcagtggtggcgtcgtgttcagcagcgatcgagaccaacggctaattctaa cggaattacgagtatttacaaaatctattattggaaacggtgagttgagtgctagaagcgacagtgaagtgtgggagaactttagaaagcagtgataaaa
gagaactttaaataaccgaaaataatcaacaaattgccgataaagaacaattaaagtaatagctgtaaaaagctctacacttcgtatggaatttgtagtt aatttctacgactacttttgccagcaaaatagtgatagacaccattcttgatttgttcgttcatctactatgactgcgagaatttttaatgtctaggaca atgatagagcgataaaagcgactagacactcttccaaacaattagcgactagacacagactacacactcttcctagtaattgcataattttttttttaaa tttaataattaaagtcagacgataagttatcgtctgaaaagcattgaaaaagaaagcccaggcgacgctatagacatcgaactagaactagaaaaagtaa gcgtaactatgaccaagcgattttcatatcgttctctcagcaaattcaaatatacacgaatttaacgaactagtactaactataaataaagtctggcgcg cgtctttctgtttgctgcccgcccggctgatagcaacaaaatgcttgccggccgctcggtcggcataacgtagaagattataatgctgtggaaaaaacca aaacaaataatatgctggcgaagtactattttcagaatattcttttcgttgcgcgacttcggctagtgtttatcgagcacatgtagagagctacgtaatc gctattaacagagctctgttaacttaacatacatacatacatacatacatacttatgtgtgtatatgatcgtttgataagacatgaatggtgagcgtgtt aatgttgtaccgaaaagaaataaatacatacagacgtttacataaaacaaacgacgcgacagtgacgaggttgtttgctatttgtagcgataagaggcga cgcgatgtgtcgaaattttcacataaatagcccgactagctaaccagatctcattcgcatttcagcagccagcaaacaaacagttagaggcacaagatgg atttgaccaacaagaacgttattttcgttgccgctctgggcggtattggtctcgacacgagtcgggagctcgtcaagcgtaatctgaaggtgagtccaac D L T N K N V I F V A A L G G I G L D T S R E L V K R N L K cgtaatctctggctggagatgaaacgatttaaacgattctgcgtccacagaattttgtcatcttggacagagttgagaacccgactgctcttgctgagct gaaggcaataaatcccaaggtgaacatcaccttccatacctacgatgtgaccgtacccgttgctgagtcgaagaagctgctgaagaagattttcgatcag A I N. P K V N I T F H T Y D V T V P V A E S K K L L K K I F D Q ctgaaaaccgttgacattctgatcaatggtgctggtattttggatgaccatcagattgagcgcaccattgccatcaacttcactggtctggtcaacacca L K T V D I L I N G A G I L D D H Q I E R T I A I N F T G L V N T ccaccgctattttggacttttgggacaagcgtaagggcggtcctggtggcatcattgccaacatttgctccgttactggcttcaatgccatccaccaggt T T A I L D F W D K R K G G P G G I I A N I C S V T G F N A I H Q gcctgtctactcggcatccaaggctgctgttgtcagcttcacaaattccctggcggtaaggagtatagcattggattcctcttatataatattaatgaac P V Y S A S K A A V V S F T N S L A atttataatgtttttatatagaaactggctcccatcactggcgttactgcctattcgatcaaccctggcatcaccaggacccccttggtgcacactttca K L A P I T G V T A T S I N P G I T
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F
actcctggctggatgtagagccacgtgttgccgaactgctgctgtcccatccaacccagacctccgagcaatgcggtcagaactttgtcaaggccattga N S W L D V E P R V A E L L L S H P T Q T S E Q C G Q N F V K A I Eggccaacaagaatggcgccatatggaaattggatctgggcacccttgaggccattgagt*ggaccaagcactgggactcgcacatctaagcatttccacgc A N K N G A I W K L D L G T L E A I E W T K H W D S H I cattgtgataagatagacgtgtatacggaattgaacaattaatatgaagtaaggctgattgataaacgttccatgttgtcgaacgctgaatggtcgaatt tgttatttttatgaaaatatatcgaagagaattc
Nucleic Acids Research, Vol. 18, No. 21
Nucleotide sequence of the Adh lebanonensis
gene
of Drosophila
E.Juan, M.Papaceit and A.Quintana Departament de Genetica, Universitat de Barcelona, 08071 Barcelona, Spain Submitted October 3, 1990
D. lebanonensis belongs to the victoria group of the subgenus Scaptodrosophila. The Adh gene was isolated from a partial BSKS+ plasmid library constructed with the 12Kb XbaI genomic fraction, the fragment XbaI-EcoRI was subcloned in BSKS (+) and BSKS (-) and a series of 14 or 12 nested deletions (obtained by the Exo-Mung method) for the non-coding and coding strand respectively were single strand sequenced with T7 DNA polymerase (Sequenase). The two introns in the coding region were unambiguously identified since the protein has been sequenced (Villarroya et al., 1989). Three putative TATA boxes have been identified, one of them with a change in the sequence,
1 101 201 301 401 501 601 701 801 901 1001 1101 1201 1301 1401 1501 1601 1701 1801 1901 2001 2101 2201
2301 2401
2501 2601 2701
2801 2901 3001
EMBL accession
no.
X53429
compared to the Drosophila species with only one Adh gene which have two promoters. To assign the initiation start sites correctly mRNA analysis is in progress. ACKNOWLEDGEMENT Supported by DGICYT PB87-0136 REFERENCE 1. Vilarroya,A., Juan,E., Egestad,B. and Jomvall,H. (1989) Eur. J. Biochem. 180, 191-197.
tcgaggtcctagaaacacactcagtgcsccctagttgagaaactgtactcgtaagtcaagtgctgacaataatctacacacatctacttgtacatattta caaatacgttgcatcagcgactctcagjagcacatgctcgtattacatatttacatacgagtatgtactaagtacatatatagtacatagtatgcgaacac acagcactatacaagtataatgcaaactacatttatacttgt.atgtaaatgagaaatctactcttttcttggcattgagcactggcgtccaatctgttgg cacagagctctgctggcatcaaataccggtcattcccattcctaggcatgttacaattattggatttgcatacctccgctctattgccatataagcttag tttttgtgctcagccttgagtggttcgctattaaaaaggcagctgctcacatcccctggatgactggggtcggctctggtacaatgtgcacctactaggg gcctgtttgtgagctcggcgcacccccaatcttttaaattgaaggtgatcttctggtcgctctgttgctgccctgcgcttatttctttgacattcactga aggtgaaaacagcatgtgccgggcccggcgggcccggacccgagcgccggtcgcttgtttacatatagttcatacacatacgatacatacgagtatgtac atacgtgggcaattgtataagcattattacaaaatatagaatgcaaaatacacacgagtcgtcgggttggcgatgctttcagctcttccgcgttcatctg gctgggtatttaaagcgcgttataccctaggacgacgattattgtcagtgcagtggtggcgtcgtgttcagcagcgatcgagaccaacggctaattctaa cggaattacgagtatttacaaaatctattattggaaacggtgagttgagtgctagaagcgacagtgaagtgtgggagaactttagaaagcagtgataaaa
gagaactttaaataaccgaaaataatcaacaaattgccgataaagaacaattaaagtaatagctgtaaaaagctctacacttcgtatggaatttgtagtt aatttctacgactacttttgccagcaaaatagtgatagacaccattcttgatttgttcgttcatctactatgactgcgagaatttttaatgtctaggaca atgatagagcgataaaagcgactagacactcttccaaacaattagcgactagacacagactacacactcttcctagtaattgcataattttttttttaaa tttaataattaaagtcagacgataagttatcgtctgaaaagcattgaaaaagaaagcccaggcgacgctatagacatcgaactagaactagaaaaagtaa gcgtaactatgaccaagcgattttcatatcgttctctcagcaaattcaaatatacacgaatttaacgaactagtactaactataaataaagtctggcgcg cgtctttctgtttgctgcccgcccggctgatagcaacaaaatgcttgccggccgctcggtcggcataacgtagaagattataatgctgtggaaaaaacca aaacaaataatatgctggcgaagtactattttcagaatattcttttcgttgcgcgacttcggctagtgtttatcgagcacatgtagagagctacgtaatc gctattaacagagctctgttaacttaacatacatacatacatacatacatacttatgtgtgtatatgatcgtttgataagacatgaatggtgagcgtgtt aatgttgtaccgaaaagaaataaatacatacagacgtttacataaaacaaacgacgcgacagtgacgaggttgtttgctatttgtagcgataagaggcga cgcgatgtgtcgaaattttcacataaatagcccgactagctaaccagatctcattcgcatttcagcagccagcaaacaaacagttagaggcacaagatgg atttgaccaacaagaacgttattttcgttgccgctctgggcggtattggtctcgacacgagtcgggagctcgtcaagcgtaatctgaaggtgagtccaac D L T N K N V I F V A A L G G I G L D T S R E L V K R N L K cgtaatctctggctggagatgaaacgatttaaacgattctgcgtccacagaattttgtcatcttggacagagttgagaacccgactgctcttgctgagct gaaggcaataaatcccaaggtgaacatcaccttccatacctacgatgtgaccgtacccgttgctgagtcgaagaagctgctgaagaagattttcgatcag A I N. P K V N I T F H T Y D V T V P V A E S K K L L K K I F D Q ctgaaaaccgttgacattctgatcaatggtgctggtattttggatgaccatcagattgagcgcaccattgccatcaacttcactggtctggtcaacacca L K T V D I L I N G A G I L D D H Q I E R T I A I N F T G L V N T ccaccgctattttggacttttgggacaagcgtaagggcggtcctggtggcatcattgccaacatttgctccgttactggcttcaatgccatccaccaggt T T A I L D F W D K R K G G P G G I I A N I C S V T G F N A I H Q gcctgtctactcggcatccaaggctgctgttgtcagcttcacaaattccctggcggtaaggagtatagcattggattcctcttatataatattaatgaac P V Y S A S K A A V V S F T N S L A atttataatgtttttatatagaaactggctcccatcactggcgttactgcctattcgatcaaccctggcatcaccaggacccccttggtgcacactttca K L A P I T G V T A T S I N P G I T
R
T
P
L
V
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T
F
actcctggctggatgtagagccacgtgttgccgaactgctgctgtcccatccaacccagacctccgagcaatgcggtcagaactttgtcaaggccattga N S W L D V E P R V A E L L L S H P T Q T S E Q C G Q N F V K A I Eggccaacaagaatggcgccatatggaaattggatctgggcacccttgaggccattgagt*ggaccaagcactgggactcgcacatctaagcatttccacgc A N K N G A I W K L D L G T L E A I E W T K H W D S H I cattgtgataagatagacgtgtatacggaattgaacaattaatatgaagtaaggctgattgataaacgttccatgttgtcgaacgctgaatggtcgaatt tgttatttttatgaaaatatatcgaagagaattc
