.=) 1990 Oxford University Press
3644 Nucleic Acids Research, Vol. 18, No. 12
Nucleotide sequence of the psaC gene of the cyanobacteriumn Synechococcus vulcanus T.Shimizu, T.Hiyama*, M.lkeuchi1, H.Koike1 and Y.lnoue1 Department of Biochemistry, Saitama University, Urawa 338 and 'Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351 -01, Japan DDBJ accession no. D00590
Submitted May 11, 1990 The psaC is found in chloroplast genomes of higher plants, tobacco (1), liverwort (2), barley (3), maize (4), pea (5) and wheat (5). It encodes a photosystem-I associated polypeptide, designated as Subunit VII, 9 kDa subunit, PsaC, etc., presumably the apoprotein for iron-sulfur clusters, Centers A and B. We previously reported a partial N-terminal amino acid sequence of Subunit VII of a thermophilic cyanobacterium, Synechococcus vulcanus (6). Here, we report the isolation and the nucleotide sequence of a Synechococcus genomic library cloned into lambdadash, which has homology to a tobacco psaC DNA probe. Nucleotide sequencing of a fragment revealed that the Synechococcus vulcanus psaC gene consists of 81 codons, the same length as the tobacco gene that we previously reported (1) as well as other higher plants (2-5). Some of the features include: the -35 box, 34-39; the -10 box, 57-62; the Shine -Dalgarno sequence, 115-119. The psaC gene starts from 130 and ends at 372. The deduced amino acid sequence indicates an 87.5% homology compared with the tobacco sequence. The nucleotide sequence homology is 73.2% of that of the tobacco gene. The GC content of this thermophilic prokaryote gene is
54.1 % which is considerably higher than those of mesophilic plants (less than 42%, calculated from ref. 1 -5).
ACKNOWLEDGEMENTS The tobacco chloroplast genomic library was kindly provided by Prof. M.Sugiura, Nagoya University. The work was supported partly by a grant from the Japanese Ministry of Education, Science and Culture (No. 01621503) to T.H. and by a grant on Solar Energy Conversion by Means of Photosynthesis awarded to RIKEN by the Science and Technology Agency of Japan.
REFERENCES 1. 2. 3. 4. 5. 6.
Hayashida,N. et al. (1987) Curr. Gen. 12, 247-250. Oh-oka,K. et al. (1987) FEBS Lett. 218, 52-54. Hoj,P.B. et al. (1987) J. Biol. Chem. 262, 12676-12684. Schantz,R. and Bogorad,L. (1988) Ptant Mol. Biol. 11, 239-247. Dunn,P.P.J. and Gray,J.C. (1988) Plant Mol. Biol. 11, 311-319. Koike,H. et al. (1989) FEBS Lett. 253, 257-263.
1 GAGTCACTGCGGTATCCTAAATCTCGGTATTTCTCTCCAGACTGCCCTTATTGTCGTAGCTTAGGGGCTTCCTGAGTTGGCGGCAGGGCA
91 TCCACCTGTCATCCTGATCGTTTTAGGAGCGGTTACTACATGGCTCACACTGTCAAAATTTACGATACTTGCATTGGCTGCACCCAGTGT HetAlaHisThrValLysIleTyrAspThrCysIleGlyCysThrGlnCys
181 GTGCGGGCCTGCCCCACCGATGTGCTGGAAATGGTGCCTTGGGATGGTTGCAAAGCTGGCCAAATTGCCTCCTCCCCTCGCACGGAAGAT
ValArgAlaCysProThrAspValLeuGluMetValProTrpAspGlyCysLysAlaGlyGlnIleAlaSerSerProArgThrGluAsp 271 IGCGTTGGCTGCAAACGCTGTGAAACGGCCTGCCCAACTGACTTTTTGAGTATTCGTGTCTAMGGGTGCAGAAACCACCCGCAGCATG
CysVa1G1yCysLysArgCysGluThrAlaCysProThrAspPheLeuSerIleArgValTyrLeuGlyAlaGluThrThrArgSerMfet 361 GGTCTAGCCTACTAGTCCCCTATTTTCGTGAAACTTTCGGCAAATTCTTGACTAGGCTTGAGGCAACATCCCTACGTCTCAAGTCTTTTA
GlyLeuAlaTyr*** 451 TTTGTTTATTGGTTGTCCTTAGCACCAACCAACCCCTACTCACCTACAACTCAGGGCTGGTCTTCGTTACTAAGGCGGCTTTGTAGGTCA 541 TCCACTGCTTCATTGATTTGGGCAATT-TAGCCTCTAGCTCTAGGCGCATCGCCTCCATCACAGGGTCATCATTCCCCTGCAGGGGAGTG 631 GATCGCTGATCTTTAATCCGTGGCCTGTTGTCCGTGGCGGCGGCGGCAACTCTCTCCTTGAGTAGGAGCGTGACCAGAGAGCCAGTGATG 721 CCGCCAAAGAAGGCGCCCACCAGAAACCCCCAGCCAAACCCATTGCCACGATT *
To whom correspondence should be addressed
3644 Nucleic Acids Research, Vol. 18, No. 12
Nucleotide sequence of the psaC gene of the cyanobacteriumn Synechococcus vulcanus T.Shimizu, T.Hiyama*, M.lkeuchi1, H.Koike1 and Y.lnoue1 Department of Biochemistry, Saitama University, Urawa 338 and 'Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351 -01, Japan DDBJ accession no. D00590
Submitted May 11, 1990 The psaC is found in chloroplast genomes of higher plants, tobacco (1), liverwort (2), barley (3), maize (4), pea (5) and wheat (5). It encodes a photosystem-I associated polypeptide, designated as Subunit VII, 9 kDa subunit, PsaC, etc., presumably the apoprotein for iron-sulfur clusters, Centers A and B. We previously reported a partial N-terminal amino acid sequence of Subunit VII of a thermophilic cyanobacterium, Synechococcus vulcanus (6). Here, we report the isolation and the nucleotide sequence of a Synechococcus genomic library cloned into lambdadash, which has homology to a tobacco psaC DNA probe. Nucleotide sequencing of a fragment revealed that the Synechococcus vulcanus psaC gene consists of 81 codons, the same length as the tobacco gene that we previously reported (1) as well as other higher plants (2-5). Some of the features include: the -35 box, 34-39; the -10 box, 57-62; the Shine -Dalgarno sequence, 115-119. The psaC gene starts from 130 and ends at 372. The deduced amino acid sequence indicates an 87.5% homology compared with the tobacco sequence. The nucleotide sequence homology is 73.2% of that of the tobacco gene. The GC content of this thermophilic prokaryote gene is
54.1 % which is considerably higher than those of mesophilic plants (less than 42%, calculated from ref. 1 -5).
ACKNOWLEDGEMENTS The tobacco chloroplast genomic library was kindly provided by Prof. M.Sugiura, Nagoya University. The work was supported partly by a grant from the Japanese Ministry of Education, Science and Culture (No. 01621503) to T.H. and by a grant on Solar Energy Conversion by Means of Photosynthesis awarded to RIKEN by the Science and Technology Agency of Japan.
REFERENCES 1. 2. 3. 4. 5. 6.
Hayashida,N. et al. (1987) Curr. Gen. 12, 247-250. Oh-oka,K. et al. (1987) FEBS Lett. 218, 52-54. Hoj,P.B. et al. (1987) J. Biol. Chem. 262, 12676-12684. Schantz,R. and Bogorad,L. (1988) Ptant Mol. Biol. 11, 239-247. Dunn,P.P.J. and Gray,J.C. (1988) Plant Mol. Biol. 11, 311-319. Koike,H. et al. (1989) FEBS Lett. 253, 257-263.
1 GAGTCACTGCGGTATCCTAAATCTCGGTATTTCTCTCCAGACTGCCCTTATTGTCGTAGCTTAGGGGCTTCCTGAGTTGGCGGCAGGGCA
91 TCCACCTGTCATCCTGATCGTTTTAGGAGCGGTTACTACATGGCTCACACTGTCAAAATTTACGATACTTGCATTGGCTGCACCCAGTGT HetAlaHisThrValLysIleTyrAspThrCysIleGlyCysThrGlnCys
181 GTGCGGGCCTGCCCCACCGATGTGCTGGAAATGGTGCCTTGGGATGGTTGCAAAGCTGGCCAAATTGCCTCCTCCCCTCGCACGGAAGAT
ValArgAlaCysProThrAspValLeuGluMetValProTrpAspGlyCysLysAlaGlyGlnIleAlaSerSerProArgThrGluAsp 271 IGCGTTGGCTGCAAACGCTGTGAAACGGCCTGCCCAACTGACTTTTTGAGTATTCGTGTCTAMGGGTGCAGAAACCACCCGCAGCATG
CysVa1G1yCysLysArgCysGluThrAlaCysProThrAspPheLeuSerIleArgValTyrLeuGlyAlaGluThrThrArgSerMfet 361 GGTCTAGCCTACTAGTCCCCTATTTTCGTGAAACTTTCGGCAAATTCTTGACTAGGCTTGAGGCAACATCCCTACGTCTCAAGTCTTTTA
GlyLeuAlaTyr*** 451 TTTGTTTATTGGTTGTCCTTAGCACCAACCAACCCCTACTCACCTACAACTCAGGGCTGGTCTTCGTTACTAAGGCGGCTTTGTAGGTCA 541 TCCACTGCTTCATTGATTTGGGCAATT-TAGCCTCTAGCTCTAGGCGCATCGCCTCCATCACAGGGTCATCATTCCCCTGCAGGGGAGTG 631 GATCGCTGATCTTTAATCCGTGGCCTGTTGTCCGTGGCGGCGGCGGCAACTCTCTCCTTGAGTAGGAGCGTGACCAGAGAGCCAGTGATG 721 CCGCCAAAGAAGGCGCCCACCAGAAACCCCCAGCCAAACCCATTGCCACGATT *
To whom correspondence should be addressed
