Nucleic Acids Research, Vol. 18, No. 14 4243
Nucleotide pertussis
sequence
of the recA
gene
of Bordetella
Didier Favre* and Jean-Franqois Viret Swiss Serum and Vaccine Institute, PO Box 2707, 3001 Berne, Switzerland Submitted June 19, 1990
EMBL accession
ACKNOWLEDGEMENT We thank J.Jelk for skilful technical assistance. REFERENCES 1. Walker,G.C. (1984) Microbiol. Rev. 48, 60-93. 2. Sancar,A., Stachelek,C., Koningsberg,W. and Rupp,W.D. (1980) Proc. Natl. Acad. Sci. USA 77, 2611-2615. 3. Wang,W.B. and Tessman,E.S. (1986) J. Bacteriol. 168, 901-910.
t;AUUATlA Xl;TT;SCACT;lAGG lTATlACGTG;GAAATATAT ATCGGElTITT ATCC CCAATGCCATAATCGCAAAAGc ;TTCGAGITlil
t:T(GAC,ACGCTGCTGCTTCGCACCCT TGCAGGCGCCGGCTTCAATACAGg§ CTTTACATGGACGACAAAACCAGCAAGGCCGCCGC'GGCCGAGAAAGCCAAGGCGCTGGCC M D D K T S X. A A Ak A E K A K TA.L A GCCGCGCTGTCGCAGATCGAAAAGCAGTTCGGCAALGGGCTCGATCATGCGCTATGGCGAC A
L
S O I
E
K O F
G
KC
G
S
I
N
R
Y
fz
D
L
D
I
A
AACGAGGTCGAACACGACATCCAGGTCGTGTCCAC'CGGTTCGCTGGGGCTGGACATCGCG N
E
V
E
H
D
I
Q
V
V
S
Tr
G
S
LG
CTGGGCGTCGGCGGCCTGCCGGCAGCCGTGATCGA LGGTCTACGGCCCCGAGTCGTCCGGC L G V G G L P A A V I E V Y G P E S S G AAGACCACGCTCACGCTGCAGGTCATCGCCGAAAT 'GCAGAAGCTGGGCGGCACCTGCGCC K T T L T L O V IA E M Q K L G G T C A TTCGTCGACGCCGAACACGCGCTGGACGTGCAATA LCGCCTCCAAGCTGGGGGTCAACCTG F
V
D
A
E
H
A
L
D
V
Q
Xr A S K L G V N L
ACCGACCTGCTGATCTCCCAGCCGGACACGGGCGA LGCAGGCCCTCGAGATCACCGACGCG T
D
L
L
I
S
O P
D
T
G
E
O
A
L
E
I
T
D
N O
I
R M K
I
A
V
M
To whom correspondence should be addressed
300 360 420 480 540
600 660
720
780 840
F
G N P E T T T G GGCAACGCGCTCAAGTTCTATTCCTCGGTGCGCCT 'GGACATCCGCCGCATCGGCGCCATC G N A L K F Y S S V R L D I R R I G A I AAGAAGGGCGACGAGGTGGTCGGCAACGAAACCCG;CGTCAAGGTGGTCAAGAACAAGGTG K K G D E V V G N E T R V K V V K N K V GCGCCGCCGTTCAAGCAGGCCGAGTTCGACATCAT 'GTATGGCAGCGGCATCTCGCGCGAA A P P F K O A E F D I N Y G S G I S R E GGCGAAATCATCGACCTGGGCGTGCAAGCCAATGT "GGTCGACAAGTCCGGCGCCTGGTAC G E I I D L G V Q A N V V D K S G A W Y AGCTACAGCGGCAACCGCATCGGCCAGGGCAAGGA *CAATGTGCGCGAGTACCTGAAAGAG S Y S G N R I G O G K D V R E Y L K E CACAAGGAAATGGCCATCGAGATCGAAAACAAGGT'GCGCGAGAACCAGGGCATAGTCAGC H K E M A I E I E N K V R E N Q G I V S CGCGCCGCCACCTTTCCCGCCAGCGAAGCCGAAGA LCGGCGAATAAGCCGTCAGGCCTGCA R A A T F P A S E A E D G E * TCCACACCCACTCCGGCATGGCGACTTTCAACCGC'CGTCCGCCATCCGCC
*
60 120 180 240
A
CTGGTGCGCTCGGGCTCGGTCGACCTGATCGTCA ?CGACTCGGTCGCGGCGCTGGTGCCC L V R S G S V D L I V I D S V A A L V P AAGGCCGAAATCGAAGGCGAGATGGGCGACTCCCT"GCCGGGCCTGCAGGCCCGCCTGATG K A E I E G E M G D S L P GL Q A R L M AGCCAGGCGCTGCGCAAGCTGACGGCCACCATCAALGCGCACCAACTGCATGGTCATCTTC S O A L R K L T A T I K R T N C N V I F ATCAACCAGATCCGCATGAAGATCGCCGTGATGTT'CGGCAACCCCGAAACCACCACCGGC I
X53457
protein known to be responsible for single-stranded DNA and/or NTP binding (3) are largely conserved in the B. pertussis protein.
The nucleotide sequence of the recA gene of Bordetella pertussis was determined. The ORF is 1065 base pairs long, encoding 352 amino acids. As expected from the high G+C content of the gene (64.2%), the codon usage is quite different from that of its E. coli counterpart. Underlined regions upstream from the gene delineate a potential ribosome-binding-site and, further upstream, putative -10 and -35 promoter sequences. The latter encompass a typical LexA-binding-site (boxed) with 15 out of 20 conserved base pairs with respect to the E. coli consensus for SOS genes (1). The RecA protein of B. pertussis has a deduced Mr of 37736, a value close to that of the E. coli RecA protein. Its predicted amino acid sequence is 63.1 % identical to the latter (identical amino acid residues are underlined) (2). Regions of the E. coli
A
no.
900 960 1020
1080 1140
1200 1260 1310
Nucleotide pertussis
sequence
of the recA
gene
of Bordetella
Didier Favre* and Jean-Franqois Viret Swiss Serum and Vaccine Institute, PO Box 2707, 3001 Berne, Switzerland Submitted June 19, 1990
EMBL accession
ACKNOWLEDGEMENT We thank J.Jelk for skilful technical assistance. REFERENCES 1. Walker,G.C. (1984) Microbiol. Rev. 48, 60-93. 2. Sancar,A., Stachelek,C., Koningsberg,W. and Rupp,W.D. (1980) Proc. Natl. Acad. Sci. USA 77, 2611-2615. 3. Wang,W.B. and Tessman,E.S. (1986) J. Bacteriol. 168, 901-910.
t;AUUATlA Xl;TT;SCACT;lAGG lTATlACGTG;GAAATATAT ATCGGElTITT ATCC CCAATGCCATAATCGCAAAAGc ;TTCGAGITlil
t:T(GAC,ACGCTGCTGCTTCGCACCCT TGCAGGCGCCGGCTTCAATACAGg§ CTTTACATGGACGACAAAACCAGCAAGGCCGCCGC'GGCCGAGAAAGCCAAGGCGCTGGCC M D D K T S X. A A Ak A E K A K TA.L A GCCGCGCTGTCGCAGATCGAAAAGCAGTTCGGCAALGGGCTCGATCATGCGCTATGGCGAC A
L
S O I
E
K O F
G
KC
G
S
I
N
R
Y
fz
D
L
D
I
A
AACGAGGTCGAACACGACATCCAGGTCGTGTCCAC'CGGTTCGCTGGGGCTGGACATCGCG N
E
V
E
H
D
I
Q
V
V
S
Tr
G
S
LG
CTGGGCGTCGGCGGCCTGCCGGCAGCCGTGATCGA LGGTCTACGGCCCCGAGTCGTCCGGC L G V G G L P A A V I E V Y G P E S S G AAGACCACGCTCACGCTGCAGGTCATCGCCGAAAT 'GCAGAAGCTGGGCGGCACCTGCGCC K T T L T L O V IA E M Q K L G G T C A TTCGTCGACGCCGAACACGCGCTGGACGTGCAATA LCGCCTCCAAGCTGGGGGTCAACCTG F
V
D
A
E
H
A
L
D
V
Q
Xr A S K L G V N L
ACCGACCTGCTGATCTCCCAGCCGGACACGGGCGA LGCAGGCCCTCGAGATCACCGACGCG T
D
L
L
I
S
O P
D
T
G
E
O
A
L
E
I
T
D
N O
I
R M K
I
A
V
M
To whom correspondence should be addressed
300 360 420 480 540
600 660
720
780 840
F
G N P E T T T G GGCAACGCGCTCAAGTTCTATTCCTCGGTGCGCCT 'GGACATCCGCCGCATCGGCGCCATC G N A L K F Y S S V R L D I R R I G A I AAGAAGGGCGACGAGGTGGTCGGCAACGAAACCCG;CGTCAAGGTGGTCAAGAACAAGGTG K K G D E V V G N E T R V K V V K N K V GCGCCGCCGTTCAAGCAGGCCGAGTTCGACATCAT 'GTATGGCAGCGGCATCTCGCGCGAA A P P F K O A E F D I N Y G S G I S R E GGCGAAATCATCGACCTGGGCGTGCAAGCCAATGT "GGTCGACAAGTCCGGCGCCTGGTAC G E I I D L G V Q A N V V D K S G A W Y AGCTACAGCGGCAACCGCATCGGCCAGGGCAAGGA *CAATGTGCGCGAGTACCTGAAAGAG S Y S G N R I G O G K D V R E Y L K E CACAAGGAAATGGCCATCGAGATCGAAAACAAGGT'GCGCGAGAACCAGGGCATAGTCAGC H K E M A I E I E N K V R E N Q G I V S CGCGCCGCCACCTTTCCCGCCAGCGAAGCCGAAGA LCGGCGAATAAGCCGTCAGGCCTGCA R A A T F P A S E A E D G E * TCCACACCCACTCCGGCATGGCGACTTTCAACCGC'CGTCCGCCATCCGCC
*
60 120 180 240
A
CTGGTGCGCTCGGGCTCGGTCGACCTGATCGTCA ?CGACTCGGTCGCGGCGCTGGTGCCC L V R S G S V D L I V I D S V A A L V P AAGGCCGAAATCGAAGGCGAGATGGGCGACTCCCT"GCCGGGCCTGCAGGCCCGCCTGATG K A E I E G E M G D S L P GL Q A R L M AGCCAGGCGCTGCGCAAGCTGACGGCCACCATCAALGCGCACCAACTGCATGGTCATCTTC S O A L R K L T A T I K R T N C N V I F ATCAACCAGATCCGCATGAAGATCGCCGTGATGTT'CGGCAACCCCGAAACCACCACCGGC I
X53457
protein known to be responsible for single-stranded DNA and/or NTP binding (3) are largely conserved in the B. pertussis protein.
The nucleotide sequence of the recA gene of Bordetella pertussis was determined. The ORF is 1065 base pairs long, encoding 352 amino acids. As expected from the high G+C content of the gene (64.2%), the codon usage is quite different from that of its E. coli counterpart. Underlined regions upstream from the gene delineate a potential ribosome-binding-site and, further upstream, putative -10 and -35 promoter sequences. The latter encompass a typical LexA-binding-site (boxed) with 15 out of 20 conserved base pairs with respect to the E. coli consensus for SOS genes (1). The RecA protein of B. pertussis has a deduced Mr of 37736, a value close to that of the E. coli RecA protein. Its predicted amino acid sequence is 63.1 % identical to the latter (identical amino acid residues are underlined) (2). Regions of the E. coli
A
no.
900 960 1020
1080 1140
1200 1260 1310
