Nucleic Acids Research, Vol. 18, No. 13 3991

.=) 1990 Oxford University Press

Nucleotide cDNA

sequence

of the rice cytoplasmic aldolase

Soh Hidaka*, Kohichi Kadowaki, Ken-ichi Tsutsumi1 and Kiichi lshikawa1 Department of Molecular Biology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki and 'Department of Biochemistry, Yamagata University School of Medicine, lida-nishi, Yamagata 990-23, Japan EMBL accession

Submitted June 2, 1990 The cDNA coding for a cytoplasmic aldolase (cALD) of rice plant (Oryza sativa L. cv. Nihonbare) was cloned by screening from rice cDNA library with a cDNA probe (pMX71) specific to the maize cALD, kindly supplied by Freeling,M. (1). The sequence of two overlapping cDNA clones contained an open reading frame coding for a protein of 358 amino acids. Of the deduced amino acid sequence, 92% was identical with that of the maize cALD (2) by considering an insertion (residue 342-344) at the C-terminal region (residue 342 of the maize cALD).

REFERENCES 1. Hake,S., Kelly,P.M., Taylor,W.C. and Freeling,M. (1985) J. Biol. Chem. 260, 5050-5054. 2. Kelly,P.M. and Tolan,D.R. (1986) Plant Physiol. 82, 1076-1080.

no.

305

X53130 NI

S

A

GATCTCCCTATTCGTCGTAGCCTCCTCCCTCGTTCGCTCCGCCCGCAACCATGTCGGCC *1 60

Y C G K Y h D E L I K N A .A Y I G T P G TACTGCGGCAA(;TACAAGG ACGAGCTCATCAAGAACGCTGCCTACATTGGCACTCCTGGC

T

T

G

K

R

F

A

S

1

2.1 120

1 (G 1 L A A I) A AGGGTATCCTTGC TGCGG.

*1.1 I1R0

A\('GTCC(;A(;AACG(TTGAGGA(;AACCGC('CGC'TCCCT('CG('GAGCTCCTCTTCTGCACCCCT

2

64 40

IN

V

F

N

V

F.

E

S

N

R

G

TGAG'I'CCACTGGCCAC('A'TTGGCAAGCGCTTCGCCAGCATC

E

R

S

L

R

E

L

L

F

C

T

P

A I L S G V I. F E E T L Y Q K T L Q iCGCCCTCCAGTrACCTCAG(: GGCGTGAI'CCT('TTCGAGGAGACCCTGTACCAGAAGACC

G G

1)

V

l;

F

T

E

*

G

K

E

V'

L

P

G

I

84 300

K D C K P F \ AAGGATGGC'AAG( CC 1'1'('G1''

1(1 360

GTCGACAAGGGCACAATCGAGGTTGCCGGCACCGAGAAGGAGACCACCACCCAGGGCCAT

124 420

GATGACCTrTGGCAAGAGATGCGCCAAGTACTACGAGGCTGOTGCCCGCTTCGCCAAGTGG

114 480

CGCGCCGTCCTCAAGATCGGCCCCAACGAGCCGTCGCAGCTCGCCATTCATCTGAATGCG

164

V

G

D

T

D

D

L

G

K

R

A

V

L

K

Q

G

L

A

E

I

L

K

I

A

E C

A

I

G

P

R

Y

A

I

L

V

D

G

P

A

A

C

Y

K

A

P

N

M

V

T

E

Y

T

V

R

T

L

S

Q

S

R

L

K

(;A\'I'(T'T'C'AJ'('A:\G(AGGGT'GTGT6TCCTCCCGGGCATCAAG

1H

C

Y

Y

E

E

P

S

I

C

Q

H

D

I

N

A

G

T

A

T

R

A

Q

L

E

N

G

L

D

P

C

A

E

H

H

D

A

I

V

r F

H

P

Q A

L

I

G

K

N

V

1 W A

E

540

CAGGGCCTCOCTCGCTACGCCATCATCTGCCAGGAGAACGGOCTGGTGCCGATCOTCGAG

184 600

CCCUAGATCCTTGTTGATGOCCCTCACGACATCGACCCOTGCGCCTACGTCTCGGAGGTO

204 660

GTCCTCOCTGCOTGCTACAAGGCCCTCAACGAGCACCATGTCCTCCTCGAGGOTACCCTC

224 720

CTCAAGCCCAACATGGTCACCCCAGGCTCTGACGCCAAGAAGGTGTCTCCCGAGGTCATC

244

P

V

L

A

P

L

L

G

N

S

Q

R

E

E

T

V

K

P

V

K

A

L

V

A

Y L

S

V

V

E

P

P

S

G

E A

E

T

V

I

V

L

I

V

780

GCTGAGTACACCGTCCGCACCCTCCAGAGGACCGTCCCCGCTGCCGTGCCCGCCATCGTC

264 840

TTCCTOTCCGGTGGGCAGAGCAAGGAGGAGGCGACCCGTAACCTCAACGCCATGAACAAG

284 900

304

F

L

T

K

K

P

L

K

A

W

S

R

C

K

A

N

A

S

S

W

K

S

L

S

A

F

T

S

R

F

N

G

L

R

N

A

A

L

M Q

N

Q

K

S

CTCAGCACCAAGAAGCCGTGGTCGCTGTCCTTCTCCTTCGCCOTGCCCTCCAGCAGAGC T

K

A

A

N

I

E

K

A

R

A

A

F

L

960

ACGCTCAAGOCCTGOTCCGGCAAGGCGGCGAACATTGAGAAGOCCAOGGCGOCCTTCCTT

324 1020

ACCAGOTGCAAGGCCAACTCCQAGOCTACCCTCOOTACATACAAGGOTGACGCTOTCCTC

344 1080

GGCOAGGGCGCCTCCGAGAOCCTTCACGTCAAGGACTACAAGTACTGATTTGCCTTOGGA

1-140

GGTGCTOGCTACCATTCGAACATTCTGCGGCTGTTGCTTAATGTGGTTTTGATATCTAGA

1200

CGGCAATCTATGGTTATCTAAAATGTCTTGTCGTAATAATTCTGGAACCTTTTTCTGTAA

1260

CACATTACCAAATATCAATTGCCTGGATTGAAATATCGTTTTTATC

T

0

E

E

S S

E L

A

H

T V

L K

0

D

*Present address: Department of Crop Breeding, Tohoku National Agricultural Experiment Station, Morioka, Iwate 020-01, Japan

T

Y

Y K

K

0

D

A

V

L

Y