Nucleic Acids Research, Vol. 18, No. 22 6683

Nucleotide sequence of two rice prolamin cDNAs L.-F.Shyur and C.-S.Chenl 2* 'Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei and 21nstitute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529, China EMBL accession

Submitted October 18, 1990

Prolamin is one of the major storage proteins of rice seed. Two prolamin cDNA clones, pX24 and pS23, were isolated from a XZAP cDNA library constructed from immature rice (Oryza sativa L. cv Tainung 67) endosperm poly(A)+ RNA. The coding regions were 92% and 84% homologous to each other at the nucleotide and amino acid levels, respectively. The arrow between amino acid 14 and 15 indicated the predicted signal

X53856, X53857

to another rice prolamin cDNA clone (pProl4) (1, 2) but have only about 65% identity to XRM7 clone (3).

REFERENCES 1. Kim,W.T. and Okita,T.W. (1988) FEBS Len. 231, 308-310. 2. Kim,W.T. and Okita,T.W. (1988) Plant Physiol. 88, 649-655.

p3.

the site and peptide cleavage peptide cleavage site and the putative polyadenylation signal

nos

Masumura,T., Hibino,T., Kidzu,K., Mitsukawa,N., Tanaka,K. and Fujii,S. (1990)

sequences are underlined as shown below. These two encoded

Mol.

Gen.

Genet.

221,

1-7.

polypeptides are highly similar (83 -94%) in amino acid sequence CCAGCAAAATAGAAAGATCTAGTGTCCCGCAGCA AAGCATTATACAACAAAAATTTAAAAGAACTAGTGTCCTGCAACA

pX24

..

pS23

34

45

pX24 pS23

F D V L G Q S Y R M K I I F V F A L L A I A A4 C R P L Q ATGAAGATCATTTTCGTCTTTGCTCTCCTTGCTATTGCTGCATGCAGGCCTCTGCA--GTTTGATGTTTTAGGTCAAAGTTATAGG ATGAAGATCATTTTCGTCTTTGCTCTCCTTGCTATTGCTGCATGC--GCCACAGCGCAGTTTGATGTTTTAGGTCAAAATATTAGG V K I I F F M A L L A I A A C A TA Q F D V L G Q N I R

120 131

pX24

Q Y Q L Q S P V L L Q Q H V L S P Y N E F V R Q Q Y G I A CAATATCAGCTGCAGTCGCCTGTCCTGCTACAGCAACATGTGCTTAGCCCATATAATGAGTTCGTAAGGCAGCAGTATGGCATAGC CAATATCAGGTGCAGTCGCCTCTCCTGCTACAGCAACAGGTGCTTAGCCTATATAATGAGTTCGTAAGGCAGCAGTATAGCATTGC Y Q V Q S P L L L Q Q Q V L S L Y N E F V R Q Q Y S I Q A

206 217

pS23

pX24 pS23

P F L S A Q S A A F Q L RN N Q V W Q Q L A L V A Q Q S GGCAAGCCCCTTCTTGCAATCAGCTGCGTTTCAACTGAGAAACAACCAAGTCTGGCAACAGCTCGCGCTGGTGGCGCAACAATCTC GGCAAGCCCCTTCTTGCAATCAGCTGTGTTTCAACTGAGAAACAACCAAGTCTTGCAACAGCTCAGGCTGGTGGCGCAACAATCTC A S P F L Q S A V F Q L RN N Q V L Q Q L R L V A Q Q S

pX24 pS23

Y Q D I N IV Q A I A Q Q L Q L Q Q F G D L Y F D R N L ACTATCAGGACATTAACATTGTTCAGGCCATAGCGCAGCAGCTACAACTCCAGCAGTTTGGTGATCTCTACTTTGATCGGAATCTG ACTACCAGGACATTAACGTTGTCCAGGCCATAGCGCAGCAGCTACACCTCCAGCAGTTTGGCGATCTCTACATTGACCGGAATCTG Y H N Q D I V V Q A I A Q Q L H L Q Q F G D L Y I D R N L

378 389

pX24 pS23

A A Q Q A L L A F N V P S R Y G I Y P R Y Y G A P S T I T GCTCAAGCTCAAGCTCTGTTGGCTTTTAACGTGCCATCTAGATATGGTATCTACCCTAGGTACTATGGTGCACCCAGTACCATTAC GCTCAAGCGCAACGACTGTTGGCTTTTAACTTGCCATCTACATATGGTATCTACCCTAGGTACTATAGAGCACCGGGTAGTATTAC A A Q Q Y P R Y Y R A P G S I T R L L A F N L P S T Y G I

464 475

pX24 pS23

CACCCTTGGCGGTGTCTTGTAATGAGTTTTAACAGTATAGTGGTTCGGAAGATAAAA-ATAAGC-TCAGATATCATCATATGTGAC CACCCTTGGCGGTGTCTTGTACTGAATTTTCACAATATTGTAGTTCGGAAGTGAAAATATAAGCCTCAGGTATCATCGTATGTGAC

292

303

H

T

T

*

L

L

G

G

G

G

V

V

L

L

*

Y

-------

550 561

*-----

pX24

ATGTGAAACTTTGGGTGATATAAATAGAAAAAAAGTTGTCTTTCATATTTAAAAAAAAAAAA ........................

612

pS23

ATGTGAAACTTAAGGTGATATAAATAGAAATAAAATTATCTTTCATATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

647

pX24 pS23

AAAAAAAAAAAAAAAAAA

665

To whom correspondence should be addressed