k.) Oxford University Press 1990
1642 Nucleic Acids Research, Vol. 18, No. 6
Nucleotide sequences of the nonstructural protein NS1 gene of three dengue-2 viruses, Ml, M2 and M3, isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome and dengue fever, respectively M.Y.Fong, C.L.Koh', S.Samuel, T.Pang and S.K.Lam Department of Medical Microbiology and 'Department of Genetics and Cellular Biology, University of Malaya, 59100 Kuala Lumpur, Malaysia EMBL accession nos X17338, X17339, X17340
Submitted January 20, 1990
The nucleotide sequences encoding the NS 1 protein of three dengue-2 (DEN-2) viruses, MI, M2 and M3, isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome and dengue fever, respectively, were determined by direct RNA sequencing by primer extension with pentadecamer primers and the dideoxyribonucleotide chain termination method. The primers were based on the published nucleotide sequence of DEN-2 PR-159 (SI) (Hahn et al., 1988).
Ml M2 M3 Ml M2 M3
1 GAUAGUGGUUGCGUUGUGAG CUGGAAGAACAAAGAACUGA AAUGUGGCAG .................. ............... ................. . ....G.......G .......... 51 UGGGAUUUUCGUCACAGAUA ACGUGCACACAUGGACAGAA CAAUACAAGU
M1
101 UCCAACCAGAAUCCCCUUCA AAACUUGCUUCUGCUAUCCA GAAAGCUCAA ......G.....A........ ..................
M2
M3 Ml M2 M3
Ml
..........
....................
.......... A
....................
..........
............... .
..........
.
.. U..................
..........
151 GAAGAGGGUAUUUGUGGUAU CCGCUCUGUUACAAGACUGG AGAAUCUUAU . A' A.. ........C.C...... *C*C A-'C-.... ............... A ......A............. A* . 201 GUGGAAACAAAUCACCCCCG AAUUCAAUCAUAUUCUAUCA GAAAAUGAAG ..
G ..... M2.... ....................
M3
ACKNOWLEDGEMENTS
Ml
W.H.O. Regional Office for the Western Pacific, Philippines; International Development Research Centre, Canada; Ministry of Science, Technology and Environment, Malaysia; and University of Malaya, Malaysia.
Ml
M2 M3 M2
1. Hahn et al. (1988) Virology 162, 167-180.
U......... ....................
........U...... .................... ....................
M2 M3
Ml M2
REFERENCE
..........
M3
Ml M2 M3
Ml M2 M3
Ml M2 M3
Ml M2
M3
Ml M2 M3
Ml M2
M3
M1 M2 M3
M1 M2
M3 Ml
M2 M13
Ml
M2
M3
M1
M2
M3
..........
301 AAACGAUCUCUGCGGCCUCA GCCCACUGAGCUUAAGUAUU CAUGGAAAAC
M3... Ml
..
[email protected]......... ......... 251 UGAAACUAACCAUCAUGACA GGAGACAUCAAAGGAAUCAU GCAGGCAGGA * ---..................... .................... ..........
.........A
.......... .......
G'-
351 AUGGGGCAAAGCUAAAAUGC UCUCCACAGAGUCUCAUAAC CAAACCUUCC .............. .....U............. .........
.........
.....
........
U U.
401 UCAUUGAUGGCCCCGAAACA GCAGAAUGCCCCAACACAAA UAGAGCUUGG ..........
....................
....................
....................
......... ................U..
451 AAUUCAUUGGAAGUUGAAGA CUACGGCUUUGGAGUGUUCA CCACCAAUAU
U...............
..................
..........
501 AUGGCUAAAAUUGAGAGAAA AACAGGAUGCAUUUUGCGAC UCAAAACUCA .................. ......
......
......
. ...........C .......... .........
.........
..U.....
551 UGUCAGCGGCCAUAAAAGAC AACAGAGCCGUCCAUGCCGA UAUGGGUUAU ....................
....................
..........
. ...G. ......A.. ..C ......... 601 UGGAUAGAAAGUGCACUCAA UGACACAUGGAAGAUAGAGA AAGCUUCUUU ...........
...C.............. ......U............ ................... ....
...............
.........
....C...
651 CAUUGAAGUCAAAAGUUGCC ACUGGCCAAAAUCACACACC CUCUGGAGCA
.........A ......................* ....... ................
.
....................
..........
..........
701 AUGGAGUGCUAGAAAGUGAG AUGAUAAUUCCUAAGAAUUU CGCUGGACCA .................. ...........A........ ................... .....U....A....... 751 GUGUCUCAACACAACUAUAG ACCAGGCUACCAUACACAAA UAGCAGGACC ..........
........
....................
....................
..........
.................... U-...... .........U-U801 AUGGCAUUUAGGAAAGCUUG AGAUGGACUUUGAUUUCUGC GAUGGAACUA ...........U...... ........... .........
..........
............U...........................
..........
851 CAGUGGUAGUGACUGAGGAC UGCGGAAAUAGAGGACCCUC UUUGAGAACA . ..G ......U.0 .............. ........ ........ .................... .......... @X U--@ 901 ACUACUGCCUCUGGAAAACU CAUAACAGAAUGGUGCUGCC GAUCUUGCAC ...... e v@ ...@@*e ***U..u .
.......
............
.........
...U.u............. 951 AUUACCACCGCUAAGAUACA GAGGUGAGGAUGGGUGCUGG UACGGGAUGG ...................
................
...............
.........
....................
..........
...... ..........C ....
Ml 1001 AAAUCAGACCAUUGAAAGAG AAAGAAGAAAAUUUGGUUAA CUCCCUGGUC M2 M3
....................
..........
Ml 1051 ACAGCC M2 M3
...... ......
...U.....
....................
. ........ . ..........A ..
U....
1642 Nucleic Acids Research, Vol. 18, No. 6
Nucleotide sequences of the nonstructural protein NS1 gene of three dengue-2 viruses, Ml, M2 and M3, isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome and dengue fever, respectively M.Y.Fong, C.L.Koh', S.Samuel, T.Pang and S.K.Lam Department of Medical Microbiology and 'Department of Genetics and Cellular Biology, University of Malaya, 59100 Kuala Lumpur, Malaysia EMBL accession nos X17338, X17339, X17340
Submitted January 20, 1990
The nucleotide sequences encoding the NS 1 protein of three dengue-2 (DEN-2) viruses, MI, M2 and M3, isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome and dengue fever, respectively, were determined by direct RNA sequencing by primer extension with pentadecamer primers and the dideoxyribonucleotide chain termination method. The primers were based on the published nucleotide sequence of DEN-2 PR-159 (SI) (Hahn et al., 1988).
Ml M2 M3 Ml M2 M3
1 GAUAGUGGUUGCGUUGUGAG CUGGAAGAACAAAGAACUGA AAUGUGGCAG .................. ............... ................. . ....G.......G .......... 51 UGGGAUUUUCGUCACAGAUA ACGUGCACACAUGGACAGAA CAAUACAAGU
M1
101 UCCAACCAGAAUCCCCUUCA AAACUUGCUUCUGCUAUCCA GAAAGCUCAA ......G.....A........ ..................
M2
M3 Ml M2 M3
Ml
..........
....................
.......... A
....................
..........
............... .
..........
.
.. U..................
..........
151 GAAGAGGGUAUUUGUGGUAU CCGCUCUGUUACAAGACUGG AGAAUCUUAU . A' A.. ........C.C...... *C*C A-'C-.... ............... A ......A............. A* . 201 GUGGAAACAAAUCACCCCCG AAUUCAAUCAUAUUCUAUCA GAAAAUGAAG ..
G ..... M2.... ....................
M3
ACKNOWLEDGEMENTS
Ml
W.H.O. Regional Office for the Western Pacific, Philippines; International Development Research Centre, Canada; Ministry of Science, Technology and Environment, Malaysia; and University of Malaya, Malaysia.
Ml
M2 M3 M2
1. Hahn et al. (1988) Virology 162, 167-180.
U......... ....................
........U...... .................... ....................
M2 M3
Ml M2
REFERENCE
..........
M3
Ml M2 M3
Ml M2 M3
Ml M2 M3
Ml M2
M3
Ml M2 M3
Ml M2
M3
M1 M2 M3
M1 M2
M3 Ml
M2 M13
Ml
M2
M3
M1
M2
M3
..........
301 AAACGAUCUCUGCGGCCUCA GCCCACUGAGCUUAAGUAUU CAUGGAAAAC
M3... Ml
..
[email protected]......... ......... 251 UGAAACUAACCAUCAUGACA GGAGACAUCAAAGGAAUCAU GCAGGCAGGA * ---..................... .................... ..........
.........A
.......... .......
G'-
351 AUGGGGCAAAGCUAAAAUGC UCUCCACAGAGUCUCAUAAC CAAACCUUCC .............. .....U............. .........
.........
.....
........
U U.
401 UCAUUGAUGGCCCCGAAACA GCAGAAUGCCCCAACACAAA UAGAGCUUGG ..........
....................
....................
....................
......... ................U..
451 AAUUCAUUGGAAGUUGAAGA CUACGGCUUUGGAGUGUUCA CCACCAAUAU
U...............
..................
..........
501 AUGGCUAAAAUUGAGAGAAA AACAGGAUGCAUUUUGCGAC UCAAAACUCA .................. ......
......
......
. ...........C .......... .........
.........
..U.....
551 UGUCAGCGGCCAUAAAAGAC AACAGAGCCGUCCAUGCCGA UAUGGGUUAU ....................
....................
..........
. ...G. ......A.. ..C ......... 601 UGGAUAGAAAGUGCACUCAA UGACACAUGGAAGAUAGAGA AAGCUUCUUU ...........
...C.............. ......U............ ................... ....
...............
.........
....C...
651 CAUUGAAGUCAAAAGUUGCC ACUGGCCAAAAUCACACACC CUCUGGAGCA
.........A ......................* ....... ................
.
....................
..........
..........
701 AUGGAGUGCUAGAAAGUGAG AUGAUAAUUCCUAAGAAUUU CGCUGGACCA .................. ...........A........ ................... .....U....A....... 751 GUGUCUCAACACAACUAUAG ACCAGGCUACCAUACACAAA UAGCAGGACC ..........
........
....................
....................
..........
.................... U-...... .........U-U801 AUGGCAUUUAGGAAAGCUUG AGAUGGACUUUGAUUUCUGC GAUGGAACUA ...........U...... ........... .........
..........
............U...........................
..........
851 CAGUGGUAGUGACUGAGGAC UGCGGAAAUAGAGGACCCUC UUUGAGAACA . ..G ......U.0 .............. ........ ........ .................... .......... @X U--@ 901 ACUACUGCCUCUGGAAAACU CAUAACAGAAUGGUGCUGCC GAUCUUGCAC ...... e v@ ...@@*e ***U..u .
.......
............
.........
...U.u............. 951 AUUACCACCGCUAAGAUACA GAGGUGAGGAUGGGUGCUGG UACGGGAUGG ...................
................
...............
.........
....................
..........
...... ..........C ....
Ml 1001 AAAUCAGACCAUUGAAAGAG AAAGAAGAAAAUUUGGUUAA CUCCCUGGUC M2 M3
....................
..........
Ml 1051 ACAGCC M2 M3
...... ......
...U.....
....................
. ........ . ..........A ..
U....
