274 OLEATE INHIBITS EXOCRINE PANCREATIC SECRETION
SIR,-Pancreatic gland secretion is inhibited by intracolonic dogs,’ cats,2 and rats.3.4 Using volunteers (six male, two female, aged 19-68 years) we have studied the effect on oleate in
pancreatic exocrine secretion of oleate instillation at different levels of the intestine. The pancreas was first submaximally stimulated by the intravenous infusion of secretin (0.5 units kg’*i h’1) and cholecystokinin-pancreozymin (c.c.K.) (2 units kg’’ h-’). After a plateau was reached, oleic acid (1ml kg-’ bodyweight) was instilled into a selected portion (duodenum, jejunum, ileum, colon, rectum) of the gut over a 5 min period with a modified Miller-Abbott sonde. The pancreatic juice was sampled every 15 min with the Sarles’ sonde during the plateau period and after oleic acid instillation. Volume, bicarbonate output (van Slyke’s method), amylase concentration (amyloclastic method), amylase output, trypsin output (enzymatic method with N-benzoyl-Arg-naphtylamide substrate) were measured. Trypsin output was also measured by a radioimmunoassay (Prof. J. P. Felber) to be described elsehuman
where.
Effect of intraileal oleic acid on pancreatic enzyme output after C.C.K. and secretin stimulation.
Changes are significant (p
SIR,-Pancreatic gland secretion is inhibited by intracolonic dogs,’ cats,2 and rats.3.4 Using volunteers (six male, two female, aged 19-68 years) we have studied the effect on oleate in
pancreatic exocrine secretion of oleate instillation at different levels of the intestine. The pancreas was first submaximally stimulated by the intravenous infusion of secretin (0.5 units kg’*i h’1) and cholecystokinin-pancreozymin (c.c.K.) (2 units kg’’ h-’). After a plateau was reached, oleic acid (1ml kg-’ bodyweight) was instilled into a selected portion (duodenum, jejunum, ileum, colon, rectum) of the gut over a 5 min period with a modified Miller-Abbott sonde. The pancreatic juice was sampled every 15 min with the Sarles’ sonde during the plateau period and after oleic acid instillation. Volume, bicarbonate output (van Slyke’s method), amylase concentration (amyloclastic method), amylase output, trypsin output (enzymatic method with N-benzoyl-Arg-naphtylamide substrate) were measured. Trypsin output was also measured by a radioimmunoassay (Prof. J. P. Felber) to be described elsehuman
where.
Effect of intraileal oleic acid on pancreatic enzyme output after C.C.K. and secretin stimulation.
Changes are significant (p
