Letter to the Editor
13 Pereira U, Garcia-Le Gal C, Le Gal G et al. Exp Dermatol 2010: 19: 796–799. 14 Pereira U, Boulais N, Lebonvallet N et al. Br J Dermatol 2010: 163: 70–77. 15 Pereira U, Boulais N, Lebonvallet N et al. Exp Dermatol 2010: 19: 931–935. 16 Lebonvallet N, Jeanmaire C, Danoux L et al. Eur J Dermatol 2010: 20: 671–684. 17 Gotz C, Pfeiffer R, Tigges J et al. Exp Dermatol 2012: 21: 358–363.
18 Zimmermann K, Hein A, Hager U et al. Nat Protoc 2009: 4: 174–196. 19 Wellnitz S A, Lesniak D R, Gerling G J et al. J Neurophysiol 2010: 103: 3378–3388. 20 Lebonvallet N, Boulais N, Le Gall C et al. Exp Dermatol 2012: 21: 156–158.
Supporting Information Additional Supporting Information may be found in the online version of this article:
Figure S1. Graphic of neuronal activity as measured by the macropatch technique on the nerve fibres of the skin explant following 10 days of culture. The graph represents the continuous trace of record before, during and after addition of capsaicin. Three phases was showed: a phase with a basal activity before addition of capsaicin, a phase with an instable signal just after capsaicin addition and a third phase with activity characterised by the apparition of numerous additional spikes compared with the basal activity.
DOI: 10.1111/exd.12301
Letter to the Editor
www.wileyonlinelibrary.com/journal/EXD
Origin of Langerhans cells in normal skin and chronic GVHD after hematopoietic stem-cell transplantation Rafiq Andani1, Ivan Robertson2, Kelli P. A. MacDonald3, Simon Durrant4, Geoffrey R. Hill3,4 and Kiarash Khosrotehrani1 1
Experimental Dermatology Laboratory, UQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD, Australia; 2Department of Dermatology, The Royal Brisbane and Women’s Hospital, Brisbane, QLD, Australia; 3Department of Immunology, QIMR-Berghofer Medical Research Institute, Brisbane, QLD, Australia; 4Bone Marrow Transplantation Unit, The Royal Brisbane and Women’s Hospital, Brisbane, QLD, Australia Correspondence: Kiarash Khosrotehrani, MD, PhD, FACD, The University of Queensland Centre for Clinical Research, Royal Brisbane Hospital Building 71/918, Herston, QLD 4029, Australia, Tel: +61733466077, Fax: +61733465598, e-mail: [email protected]
Abstract: Chronic graft-versus-host disease (cGVHD) is a common complication following allogeneic stem-cell transplantation (SCT). Past studies have implicated the persistence of host antigen-presenting cells (APCs) in GVHD. Our objective was to determine the frequency of host Langerhans cells (LCs) in normal skin post-SCT and ask if their persistence could predict cGVHD. Biopsies of normal skin from 124 sex-mismatched T-cell-replete allogenic SCT recipients were taken 100 days posttransplant. Patients with acute GVHD and those with
13 Pereira U, Garcia-Le Gal C, Le Gal G et al. Exp Dermatol 2010: 19: 796–799. 14 Pereira U, Boulais N, Lebonvallet N et al. Br J Dermatol 2010: 163: 70–77. 15 Pereira U, Boulais N, Lebonvallet N et al. Exp Dermatol 2010: 19: 931–935. 16 Lebonvallet N, Jeanmaire C, Danoux L et al. Eur J Dermatol 2010: 20: 671–684. 17 Gotz C, Pfeiffer R, Tigges J et al. Exp Dermatol 2012: 21: 358–363.
18 Zimmermann K, Hein A, Hager U et al. Nat Protoc 2009: 4: 174–196. 19 Wellnitz S A, Lesniak D R, Gerling G J et al. J Neurophysiol 2010: 103: 3378–3388. 20 Lebonvallet N, Boulais N, Le Gall C et al. Exp Dermatol 2012: 21: 156–158.
Supporting Information Additional Supporting Information may be found in the online version of this article:
Figure S1. Graphic of neuronal activity as measured by the macropatch technique on the nerve fibres of the skin explant following 10 days of culture. The graph represents the continuous trace of record before, during and after addition of capsaicin. Three phases was showed: a phase with a basal activity before addition of capsaicin, a phase with an instable signal just after capsaicin addition and a third phase with activity characterised by the apparition of numerous additional spikes compared with the basal activity.
DOI: 10.1111/exd.12301
Letter to the Editor
www.wileyonlinelibrary.com/journal/EXD
Origin of Langerhans cells in normal skin and chronic GVHD after hematopoietic stem-cell transplantation Rafiq Andani1, Ivan Robertson2, Kelli P. A. MacDonald3, Simon Durrant4, Geoffrey R. Hill3,4 and Kiarash Khosrotehrani1 1
Experimental Dermatology Laboratory, UQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD, Australia; 2Department of Dermatology, The Royal Brisbane and Women’s Hospital, Brisbane, QLD, Australia; 3Department of Immunology, QIMR-Berghofer Medical Research Institute, Brisbane, QLD, Australia; 4Bone Marrow Transplantation Unit, The Royal Brisbane and Women’s Hospital, Brisbane, QLD, Australia Correspondence: Kiarash Khosrotehrani, MD, PhD, FACD, The University of Queensland Centre for Clinical Research, Royal Brisbane Hospital Building 71/918, Herston, QLD 4029, Australia, Tel: +61733466077, Fax: +61733465598, e-mail: [email protected]
Abstract: Chronic graft-versus-host disease (cGVHD) is a common complication following allogeneic stem-cell transplantation (SCT). Past studies have implicated the persistence of host antigen-presenting cells (APCs) in GVHD. Our objective was to determine the frequency of host Langerhans cells (LCs) in normal skin post-SCT and ask if their persistence could predict cGVHD. Biopsies of normal skin from 124 sex-mismatched T-cell-replete allogenic SCT recipients were taken 100 days posttransplant. Patients with acute GVHD and those with
