Clinical science
Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy: possible involvement of periostin Yoshiyuki Kobayashi,1 Shigeo Yoshida,1 Takahito Nakama,1 Yedi Zhou,1 Keijiro Ishikawa,1 Ryoichi Arita,1 Shintaro Nakao,1 Masanori Miyazaki,1 Yukio Sassa,1,2 Yuji Oshima,1 Kenji Izuhara,3 Toshihiro Kono,2 Tatsuro Ishibashi1 ▸ Additional material is published online only. To view please visit the journal online (http://dx.doi.org/10.1136/ bjophthalmol-2014-305321). 1
Department of Ophthalmology, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan 2 Department of Ophthalmology, Fukuoka University Chikushi Hospital, Chikusino-shi, Fukuoka, Japan 3 Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School, Saga, Japan Correspondence to Shigeo Yoshida, Department of Ophthalmology, Kyushu University Graduate School of Medical Sciences, Fukuoka, 812-8582, Japan; [email protected] Received 29 March 2014 Revised 2 September 2014 Accepted 17 September 2014 Published Online First 3 October 2014
ABSTRACT Aim To determine whether CD163, a specific marker for M2 macrophages, is involved in the formation of preretinal fibrovascular membranes (FVMs) present in eyes with proliferative diabetic retinopathy (PDR). Methods We measured the levels of soluble (s)CD163, periostin and vascular endothelial growth factor by sandwich ELISA in vitreous samples from 74 eyes of 62 patients with PDR, 20 eyes of 18 patients with proliferative vitreoretinopathy, and 56 eyes of 54 patients with non-diabetic ocular diseases (control group). Immunohistochemical analyses were performed to determine the expressions of CD68, CD163 and periostin in the surgically resected FVMs and idiopathic epiretinal membranes (ERMs). Results The concentrations of sCD163 and periostin in the vitreous were significantly higher in patients with PDR than in non-diabetic controls ( p
Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy: possible involvement of periostin Yoshiyuki Kobayashi,1 Shigeo Yoshida,1 Takahito Nakama,1 Yedi Zhou,1 Keijiro Ishikawa,1 Ryoichi Arita,1 Shintaro Nakao,1 Masanori Miyazaki,1 Yukio Sassa,1,2 Yuji Oshima,1 Kenji Izuhara,3 Toshihiro Kono,2 Tatsuro Ishibashi1 ▸ Additional material is published online only. To view please visit the journal online (http://dx.doi.org/10.1136/ bjophthalmol-2014-305321). 1
Department of Ophthalmology, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan 2 Department of Ophthalmology, Fukuoka University Chikushi Hospital, Chikusino-shi, Fukuoka, Japan 3 Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School, Saga, Japan Correspondence to Shigeo Yoshida, Department of Ophthalmology, Kyushu University Graduate School of Medical Sciences, Fukuoka, 812-8582, Japan; [email protected] Received 29 March 2014 Revised 2 September 2014 Accepted 17 September 2014 Published Online First 3 October 2014
ABSTRACT Aim To determine whether CD163, a specific marker for M2 macrophages, is involved in the formation of preretinal fibrovascular membranes (FVMs) present in eyes with proliferative diabetic retinopathy (PDR). Methods We measured the levels of soluble (s)CD163, periostin and vascular endothelial growth factor by sandwich ELISA in vitreous samples from 74 eyes of 62 patients with PDR, 20 eyes of 18 patients with proliferative vitreoretinopathy, and 56 eyes of 54 patients with non-diabetic ocular diseases (control group). Immunohistochemical analyses were performed to determine the expressions of CD68, CD163 and periostin in the surgically resected FVMs and idiopathic epiretinal membranes (ERMs). Results The concentrations of sCD163 and periostin in the vitreous were significantly higher in patients with PDR than in non-diabetic controls ( p
