Journal of Infectious Diseases Advance Access published November 26, 2014
1 Pefluorocarbon Emulsion Therapy Attenuates Pneumococcal Infection in Sickle Cell Mice
1
cr ipt
Nawal Helmi1,2,3, Peter W. Andrew1, Hitesh C. Pandya1 Department of Infection, Immunity and inflammation, Maurice Shock Building, University
of Leicester, University Road, Leicester LE1 9HN, UK 2
High Ministry of Education, Saudi Arabia
3
us
Address for Correpsondence: Dr Hitesh C Pandya MBChB MRCP MD, Room 212b, Maurice
an
Shock Building, University of Leicester, University Road, Leicester LE1 9HN, UK, Email: [email protected], Tel: +44(0) 116 252 3056, Fax: +44(0) 116 252 5030
M
Ac
ce
pt ed
© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: [email protected].
Downloaded from http://jid.oxfordjournals.org/ at New York University on February 15, 2015
Al‐Farabi College, Jeddah, Saudi Arabia
2 Abstract
cr ipt
Impaired immunity and tissue hypoxia‐ischaemia are strongly linked with Streptococcus pneumoniae pathogenesis in patients with sickle cell anaemia. Perfluorocarbon emulsions
(PFCE) have high O2 dissolving capacity and can alleviate tissue hypoxia. Here, we evaluate
the effects of intravenous PFCE therapy in transgenic sickle cell (HbSS) mice infected with S.
us
an
HbSS and C57BL/6 (control) mice intravenously infected with S. pneumoniae were treated with intravenous PFCE or PBS and then managed in either air/O2 (FiO2 = 50%, PFCE‐O2, PBS‐ O2) or air only (PFCE‐Air, PBS‐Air) gas mixtures. Lungs were processed for leukocyte and
M
bacterial counts and cytokine measurements
pt ed
HbSS mice developed severe pneumococcal infection significantly faster than C57BL/6 mice (Kaplan Maier analysis, P
1 Pefluorocarbon Emulsion Therapy Attenuates Pneumococcal Infection in Sickle Cell Mice
1
cr ipt
Nawal Helmi1,2,3, Peter W. Andrew1, Hitesh C. Pandya1 Department of Infection, Immunity and inflammation, Maurice Shock Building, University
of Leicester, University Road, Leicester LE1 9HN, UK 2
High Ministry of Education, Saudi Arabia
3
us
Address for Correpsondence: Dr Hitesh C Pandya MBChB MRCP MD, Room 212b, Maurice
an
Shock Building, University of Leicester, University Road, Leicester LE1 9HN, UK, Email: [email protected], Tel: +44(0) 116 252 3056, Fax: +44(0) 116 252 5030
M
Ac
ce
pt ed
© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: [email protected].
Downloaded from http://jid.oxfordjournals.org/ at New York University on February 15, 2015
Al‐Farabi College, Jeddah, Saudi Arabia
2 Abstract
cr ipt
Impaired immunity and tissue hypoxia‐ischaemia are strongly linked with Streptococcus pneumoniae pathogenesis in patients with sickle cell anaemia. Perfluorocarbon emulsions
(PFCE) have high O2 dissolving capacity and can alleviate tissue hypoxia. Here, we evaluate
the effects of intravenous PFCE therapy in transgenic sickle cell (HbSS) mice infected with S.
us
an
HbSS and C57BL/6 (control) mice intravenously infected with S. pneumoniae were treated with intravenous PFCE or PBS and then managed in either air/O2 (FiO2 = 50%, PFCE‐O2, PBS‐ O2) or air only (PFCE‐Air, PBS‐Air) gas mixtures. Lungs were processed for leukocyte and
M
bacterial counts and cytokine measurements
pt ed
HbSS mice developed severe pneumococcal infection significantly faster than C57BL/6 mice (Kaplan Maier analysis, P
