Biomaterials, Artificial Cells and Immobilization Biotechnology
ISSN: 1055-7172 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/ianb18
Perfluorochemical Reperfusion Limits Myocardial Reperfusion Injury After Prolonged Hypothermic Global Ischemia Stephen M. Martin, Hillel Laks, Davis C. Drinkwater, Darryl G. Stein, Steven W. Barthel, Eli R. Capouya, Jeffrey M. Pearl, Sunita Bhuta, Betty Ho & Paul Chang To cite this article: Stephen M. Martin, Hillel Laks, Davis C. Drinkwater, Darryl G. Stein, Steven W. Barthel, Eli R. Capouya, Jeffrey M. Pearl, Sunita Bhuta, Betty Ho & Paul Chang (1992) Perfluorochemical Reperfusion Limits Myocardial Reperfusion Injury After Prolonged Hypothermic Global Ischemia, Biomaterials, Artificial Cells and Immobilization Biotechnology, 20:2-4, 985-989, DOI: 10.3109/10731199209119752 To link to this article: http://dx.doi.org/10.3109/10731199209119752
Published online: 11 Jul 2009.
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Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=ianb18 Download by: [Australian National University]
Date: 27 April 2016, At: 22:48
BIOMAT., ART. C E L L S & IMMOB. B I O T E C H . ,
20(2-4), 985-989 ( 1 9 9 2 )
PERFLUOROCHEMICAL REPERFUSION LIMITS MYOCARDIAL REPERFUSION INJURY AFTER PROLONGED HYPOTHERMIC GLOBAL ISCHEMIA
Downloaded by [Australian National University] at 22:48 27 April 2016
Stephen M. Martin, Hillel Laks, Davis C. Drinkwater, Darryl G. Stein, Steven W. Barthel, Eli R. Capouya, Jeffrey M. Pearl, Sunita Bhuta, Betty Ho, and Paul Chang. Division of Cardiothoracic Surgery, University of California at Los Angeles Medical Centar, Los Angeles, CA, USA.
Abstract. The ability of an oxygenated perfluorochemical (FluosolB) to limit myocardial reperfusion injury following global hypothermic ischemic insult was investigated. Neonatal piglet hearts were arrested with cold crystalloid cardioplegia and stored for 12 hours in 20 C saline. Reperfusion was carried out using an isolated, blood-perfused, working heart preparation. Hearts were initially reperfused (10 minutes) with either whole blood (WB, n=6), unmodified perfluorochemical (PFC, n=8), or aspartate/glutamate-enriched perfluorochemical cardioplegia (PFC+, n=6). prior to institution of whole blood perfusion, functional evaluation and left ventricular biopsy. A control group (C, n=7) was evaluated without an intervening period of ischemia. At a left ventricular diastolic pressure of 9 rnm Hg WB hearts developed a left-ventricular stroke work index (SWI) of 3.8f2.3 x l o 3 erg/g (meanfstandard error of the mean). Under similar conditions, PFCreperfused hearts achieved a SWI of 14.6fl.3 x 103, significantly greater than that of WB (p
ISSN: 1055-7172 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/ianb18
Perfluorochemical Reperfusion Limits Myocardial Reperfusion Injury After Prolonged Hypothermic Global Ischemia Stephen M. Martin, Hillel Laks, Davis C. Drinkwater, Darryl G. Stein, Steven W. Barthel, Eli R. Capouya, Jeffrey M. Pearl, Sunita Bhuta, Betty Ho & Paul Chang To cite this article: Stephen M. Martin, Hillel Laks, Davis C. Drinkwater, Darryl G. Stein, Steven W. Barthel, Eli R. Capouya, Jeffrey M. Pearl, Sunita Bhuta, Betty Ho & Paul Chang (1992) Perfluorochemical Reperfusion Limits Myocardial Reperfusion Injury After Prolonged Hypothermic Global Ischemia, Biomaterials, Artificial Cells and Immobilization Biotechnology, 20:2-4, 985-989, DOI: 10.3109/10731199209119752 To link to this article: http://dx.doi.org/10.3109/10731199209119752
Published online: 11 Jul 2009.
Submit your article to this journal
Article views: 5
View related articles
Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=ianb18 Download by: [Australian National University]
Date: 27 April 2016, At: 22:48
BIOMAT., ART. C E L L S & IMMOB. B I O T E C H . ,
20(2-4), 985-989 ( 1 9 9 2 )
PERFLUOROCHEMICAL REPERFUSION LIMITS MYOCARDIAL REPERFUSION INJURY AFTER PROLONGED HYPOTHERMIC GLOBAL ISCHEMIA
Downloaded by [Australian National University] at 22:48 27 April 2016
Stephen M. Martin, Hillel Laks, Davis C. Drinkwater, Darryl G. Stein, Steven W. Barthel, Eli R. Capouya, Jeffrey M. Pearl, Sunita Bhuta, Betty Ho, and Paul Chang. Division of Cardiothoracic Surgery, University of California at Los Angeles Medical Centar, Los Angeles, CA, USA.
Abstract. The ability of an oxygenated perfluorochemical (FluosolB) to limit myocardial reperfusion injury following global hypothermic ischemic insult was investigated. Neonatal piglet hearts were arrested with cold crystalloid cardioplegia and stored for 12 hours in 20 C saline. Reperfusion was carried out using an isolated, blood-perfused, working heart preparation. Hearts were initially reperfused (10 minutes) with either whole blood (WB, n=6), unmodified perfluorochemical (PFC, n=8), or aspartate/glutamate-enriched perfluorochemical cardioplegia (PFC+, n=6). prior to institution of whole blood perfusion, functional evaluation and left ventricular biopsy. A control group (C, n=7) was evaluated without an intervening period of ischemia. At a left ventricular diastolic pressure of 9 rnm Hg WB hearts developed a left-ventricular stroke work index (SWI) of 3.8f2.3 x l o 3 erg/g (meanfstandard error of the mean). Under similar conditions, PFCreperfused hearts achieved a SWI of 14.6fl.3 x 103, significantly greater than that of WB (p
