ADDITIONS AND CORRECTIONS THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. 48, p. 28726, November 27, 2015 © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.
VOLUME 290 (2015) PAGES 11376 –11383 DOI 10.1074/jbc.A114.627778
Pharmacological inhibition of ULK1 kinase blocks mammalian target of rapamycin (mTOR)-dependent autophagy. Katy J. Petherick, Owen J. L. Conway, Chido Mpamhanga, Simon A. Osborne, Ahmad Kamal, Barbara Saxty, and Ian G. Ganley
PAGE 11378:
In Fig. 1A, the molecular structures of the compounds for MRT67307 and MRT68921 were mistakenly switched. The corrected figure is shown below. This correction does not affect the interpretation of the results or conclusions of this work.
A.
B. IC50 (nM) ULK1 ULK2 MRT67307 45.0 38.0 MRT68921 2.9 1.1
ATG13
O
N
N O H N
H N
tubulin
- 50
LC3-II
- 15
6
4
4 2
control MRT68921
+
bafilomycin
-
-
-
EBSS
EBSS MRT68921
60
ctrl
92
1
7
-
30
1
92
30
7
1
-
0
0
ctrl
D.
*
92
- 75
total
*
2
-
- 75
*
30
- 150 - 250
7
- + + + - - + + - - - +
DMSO baf.
relative LC3-II level
6
mean LC3 puncta per cell
ATG13
- + + + - - + + - - - +
- 75
total
8
ULK dKO MEFs input (7.5%) FLAG IP
FLAG-ULK1: EBSS: MRT68921: ULK1 FIP200 pS318
- 75
O
N
C.
- 150 - 150
total pS318 *
30 7 92 1
N
N H
EBSS
pS757
relative phospho-ATG13
N
ULK1
H N
H N
N
-
H N
control
MRT67307: - - + + - - - - + + - MRT68921: - - - - + + - - - - + + bafilomycin: - + - + - + - + - + - +
EBSS
DMSO baf.
50 40 n/s
*
30 20 10 0
- 921 control
-
921 EBSS
Authors are urged to introduce these corrections into any reprints they distribute. Secondary (abstract) services are urged to carry notice of these corrections as prominently as they carried the original abstracts.
28726 JOURNAL OF BIOLOGICAL CHEMISTRY
VOLUME 290 • NUMBER 48 • NOVEMBER 27, 2015
VOLUME 290 (2015) PAGES 11376 –11383 DOI 10.1074/jbc.A114.627778
Pharmacological inhibition of ULK1 kinase blocks mammalian target of rapamycin (mTOR)-dependent autophagy. Katy J. Petherick, Owen J. L. Conway, Chido Mpamhanga, Simon A. Osborne, Ahmad Kamal, Barbara Saxty, and Ian G. Ganley
PAGE 11378:
In Fig. 1A, the molecular structures of the compounds for MRT67307 and MRT68921 were mistakenly switched. The corrected figure is shown below. This correction does not affect the interpretation of the results or conclusions of this work.
A.
B. IC50 (nM) ULK1 ULK2 MRT67307 45.0 38.0 MRT68921 2.9 1.1
ATG13
O
N
N O H N
H N
tubulin
- 50
LC3-II
- 15
6
4
4 2
control MRT68921
+
bafilomycin
-
-
-
EBSS
EBSS MRT68921
60
ctrl
92
1
7
-
30
1
92
30
7
1
-
0
0
ctrl
D.
*
92
- 75
total
*
2
-
- 75
*
30
- 150 - 250
7
- + + + - - + + - - - +
DMSO baf.
relative LC3-II level
6
mean LC3 puncta per cell
ATG13
- + + + - - + + - - - +
- 75
total
8
ULK dKO MEFs input (7.5%) FLAG IP
FLAG-ULK1: EBSS: MRT68921: ULK1 FIP200 pS318
- 75
O
N
C.
- 150 - 150
total pS318 *
30 7 92 1
N
N H
EBSS
pS757
relative phospho-ATG13
N
ULK1
H N
H N
N
-
H N
control
MRT67307: - - + + - - - - + + - MRT68921: - - - - + + - - - - + + bafilomycin: - + - + - + - + - + - +
EBSS
DMSO baf.
50 40 n/s
*
30 20 10 0
- 921 control
-
921 EBSS
Authors are urged to introduce these corrections into any reprints they distribute. Secondary (abstract) services are urged to carry notice of these corrections as prominently as they carried the original abstracts.
28726 JOURNAL OF BIOLOGICAL CHEMISTRY
VOLUME 290 • NUMBER 48 • NOVEMBER 27, 2015
