Functions of PEDF in RGC-5 cells
·Basic Research·
Pigment epithelium-derived factor protects retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction Shu-Wei Tian1,2, Yuan Ren1, Jin-Zhi Pei1, Bai-Chao Ren2, Yuan He1 1
Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Medical Univeristy, Xi’an 710038, Shaanxi Province, China 2 Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710000, Shaanxi Province, China Correspondence to: Yuan He. Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Medical Univeristy, Xi’an 710038, Shaanxi Province, China. openji7127@hotmail. com; Bai-Chao Ren. Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710000, Shaanxi Province, China. [email protected] Received: 2016-09-30 Accepted: 2017-05-02
● RESULTS: SS induced differentiation of RGC-5 cells resulting in elongation of their neurites and establishing contacts between outgrowths. Exposure to 300 μmol/mL CoCl2 triggered death of 30% of the total cells in cultures within 24h. At the same time, pretreatment with 100 ng/mL PEDF significantly suppressed the cell death induced by hypoxia (P
·Basic Research·
Pigment epithelium-derived factor protects retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction Shu-Wei Tian1,2, Yuan Ren1, Jin-Zhi Pei1, Bai-Chao Ren2, Yuan He1 1
Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Medical Univeristy, Xi’an 710038, Shaanxi Province, China 2 Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710000, Shaanxi Province, China Correspondence to: Yuan He. Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Medical Univeristy, Xi’an 710038, Shaanxi Province, China. openji7127@hotmail. com; Bai-Chao Ren. Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710000, Shaanxi Province, China. [email protected] Received: 2016-09-30 Accepted: 2017-05-02
● RESULTS: SS induced differentiation of RGC-5 cells resulting in elongation of their neurites and establishing contacts between outgrowths. Exposure to 300 μmol/mL CoCl2 triggered death of 30% of the total cells in cultures within 24h. At the same time, pretreatment with 100 ng/mL PEDF significantly suppressed the cell death induced by hypoxia (P
