Plasma, Apolipoprotein A-I and A-II Levels in Hyperlipidemia GUSTAV SCHONFELD, ALBERTA BAILEY, and ROBERT STEELMAN, Lipid Research Center, Department of Preventive Medicine and Medicine, Washington University School of Medicine, Box 8046, 4566 Scott Avenue, St. Louis, Missouri 63110
ABSTRACT
Some of the component moieties of high denisty lipoproteins (HDL) were analyzed in normal subjects and in patients with hyperlipidemia. Apoproteins A-I and A-II were quantified by radioimmunoassay, HDL cholesterol and triglycerides were assessed on heparin-MnC12 supernates of fasting plasmas. We found that HDL is enriched in triglycerides in all forms of hyperlipidemia, while the proportion of ApoA-II is unaltered and the proportion of ApoA-I is decreased. Thus, the composition of HDL is altered in hypertriglyceridemia. The molecular associations of ApoA-I and ApoA-II in plasma were also examined by assaying the apoprotein contents of plasma fractions prepared by ultracentrifugation and by gel filtration column chromatography. The ApoA-1 contents of d0.3 (29), and ratios of the VLDL-ApoELIPIDS, VOL. 13, NO. 12
954
GUSTAV SCHONFELD, ALBERTA BAILEY, AND ROBERT STEELMAN T A B L E IV A p o l i p o p r o t e i n A-I a n d A - I I Levels in Hyperlipidemia (Men) a
Phenotype
ApoA-I
ApoA-II
N (19) IIa
109 +- 24
40
+- 8
108 + 37
38
• 10
124 • 44
44
-+ 7
121 -+ 26
41
•
104 • 30
36
+- 8
90 b -+ 14
30 b +- 12
(8) lib
(13) III
9
(6) IV
(17) V
a R e s u l t s are in m g / d l given as m e a n s -+ 1 S.D~ nonpaired t-tests are c a l c u l a t e d using log t r a n s f o r m e d d a t a . bp80% of the ApoA-I peak eluted with HDL in plasma with triglyceride contents of < 1 0 0 0 mg/dl. No immunologic activity due to ApoA-I was found to e l u t i o n volumes which would have corresponded to ApoA-I monomers (MW ~28,000) or fragments in either normal or hyperlipidemic plasmas.
A
V
> ;n
-
ABSTRACT
Some of the component moieties of high denisty lipoproteins (HDL) were analyzed in normal subjects and in patients with hyperlipidemia. Apoproteins A-I and A-II were quantified by radioimmunoassay, HDL cholesterol and triglycerides were assessed on heparin-MnC12 supernates of fasting plasmas. We found that HDL is enriched in triglycerides in all forms of hyperlipidemia, while the proportion of ApoA-II is unaltered and the proportion of ApoA-I is decreased. Thus, the composition of HDL is altered in hypertriglyceridemia. The molecular associations of ApoA-I and ApoA-II in plasma were also examined by assaying the apoprotein contents of plasma fractions prepared by ultracentrifugation and by gel filtration column chromatography. The ApoA-1 contents of d0.3 (29), and ratios of the VLDL-ApoELIPIDS, VOL. 13, NO. 12
954
GUSTAV SCHONFELD, ALBERTA BAILEY, AND ROBERT STEELMAN T A B L E IV A p o l i p o p r o t e i n A-I a n d A - I I Levels in Hyperlipidemia (Men) a
Phenotype
ApoA-I
ApoA-II
N (19) IIa
109 +- 24
40
+- 8
108 + 37
38
• 10
124 • 44
44
-+ 7
121 -+ 26
41
•
104 • 30
36
+- 8
90 b -+ 14
30 b +- 12
(8) lib
(13) III
9
(6) IV
(17) V
a R e s u l t s are in m g / d l given as m e a n s -+ 1 S.D~ nonpaired t-tests are c a l c u l a t e d using log t r a n s f o r m e d d a t a . bp80% of the ApoA-I peak eluted with HDL in plasma with triglyceride contents of < 1 0 0 0 mg/dl. No immunologic activity due to ApoA-I was found to e l u t i o n volumes which would have corresponded to ApoA-I monomers (MW ~28,000) or fragments in either normal or hyperlipidemic plasmas.
A
V
> ;n
-
