Platelet-Activating Factor Receptor Contributes to Antileishmanial Function of Miltefosine This information is current as of May 21, 2015.
Pallavi R. Gangalum, Waldionê de Castro, Leda Q. Vieira, Ranadhir Dey, Luis Rivas, Shailza Singh, Subrata Majumdar and Bhaskar Saha
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The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 9650 Rockville Pike, Bethesda, MD 20814-3994. Copyright © 2015 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606.
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J Immunol published online 15 May 2015 http://www.jimmunol.org/content/early/2015/05/15/jimmun ol.1401890
Published May 15, 2015, doi:10.4049/jimmunol.1401890 The Journal of Immunology
Platelet-Activating Factor Receptor Contributes to Antileishmanial Function of Miltefosine Pallavi R. Gangalum,*,1 Waldioneˆ de Castro,†,1 Leda Q. Vieira,† Ranadhir Dey,‡ Luis Rivas,x Shailza Singh,* Subrata Majumdar,‡ and Bhaskar Saha*
V
isceral leishmaniasis (VL), a fatal disease with an annual worldwide incidence of 0.5 million new cases (1), is caused by infection with the protozoan species Leishmania infantum, L. chagasi, and L. donovani, the last-named with the highest relevance in terms of mortality and number of patients. Treatment with antimonial drugs has declined rapidly with increased resistance of the parasite. One alternative is liposomal amphotericin B, which requires parenteral administration and is prohibitively expensive (2). So, a new orally bioavailable antileishmanial drug was a pressing need, when miltefosine [hexadecylphosphocholine (HPC)], an alkyl-phosphocholine originally developed as an anticancer drug (3), was proved to be an effective antileishmanial agent in clinics (4). Miltefosine is reported to
*National Centre for Cell Science, Ganeshkhind, Pune 411007, India; †Nucleo de Pesquisa em Cieˆncias Biolo´gicas, Universidade Federal de Ouro Preto, CEP 35400-000 Ouro Preto, Brazil; ‡Bose Institute, Kolkata 700 054, India; and xCentro de Investigaciones Biolo´gicas, Consejo Superior de Investigaciones Cientificas, Unidad Metabolo´ mica, Interacciones y Bioana´lisis, Universidad CEU San Pablo, 28040 Madrid, Spain 1
P.R.G. and W.d.C. share equal contribution to this work.
Received for publication July 24, 2014. Accepted for publication March 30, 2015. This work was supported by partial funding provided by Fundac¸ao de Amparo a` Pesquisa do Estado de Minas Gerais Process CBBAPQ-00082-08; L.R. was supported by Fondo de Investigaciones Sanitarias and VI PN de I+D+I 2008-2011, Instituto de Salud Carlos III PI12-02706, and Subdireccio´n General de Redes y Centros de Investigacio´n Cooperativa-FEDER RD12/0018/0007; P.R.G. and R.D. were Council of Scientific and Industrial Research fellows; W.d.C. and L.Q.V. are Conselho Nacional de Pesquisas fellows. Address correspondence and reprint requests to Dr. Bhaskar Saha, National Centre for Cell Science, Ganeshkhind, Pune 411007, India. E-mail address: sahab@nccs. res.in Abbreviations used in this article: CSA, crude soluble Ag; HPC, hexadecylphosphocholine; IM, infected macrophage; KO, knockout; MD, molecular dynamic; PAF, platelet-activating factor; UIM, uninfected macrophage; VL, visceral leishmaniasis; WT, wild-type. Copyright Ó 2015 by The American Association of Immunologists, Inc. 0022-1767/15/$25.00 www.jimmunol.org/cgi/doi/10.4049/jimmunol.1401890
trigger apoptosis-like effects (5), lipid-dependent cell signaling in cancer cells (6), and impaired phospholipid synthesis in the parasite (7), as well as acting as an immunomodulator, promoting macrophage activation (8–11). Together with our previous report on the host cell signaling–dependent antileishmanial activity of miltefosine (12), these observations imply that miltefosine may work through a cell surface receptor. HPC shared important structural features with platelet-activating factor (PAF), such as a single long fatty acyl chain and a phosphocholine polar head. Although PAF enhanced killing of L. amazonensis, some of its antagonists inhibited the parasite clearance in mouse macrophages (13–15). Accordingly, PAF receptor (PAF receptor) is perhaps the most feasible candidate to serve as an HPC receptor. Indeed, PAF receptor–deficient mice were more susceptible to L. amazonensis infection and showed a delayed IFN-g production and higher arginase activity, suggesting an essential role for PAF receptor signaling in the control of L. amazonensis infection in mice (16). Thus, consistent with its structural similarities to PAF, our hypothesis was that HPC had at least partial requirement for PAF receptor to exert its leishmanicidal function through immunomodulation of macrophages, the obligate host cell for Leishmania. Therefore, we examined whether PAF receptor blockade or PAF receptor deficiency reduced the antileishmanial functions of miltefosine. Our observations showed that an anti-PAF receptor Ab significantly reduced the miltefosine-triggered Th1 response, TNF-a production, and parasite killing in macrophages. In corroboration of these observations, miltefosine-induced L. donovani clearance was significantly impaired in PAF receptor–deficient macrophages and mice. Thus, PAF receptor contributes to miltefosine-induced antileishmanial functions. The PAF receptor–dependent component of HPC perhaps accounts for the heightened inflammation observed in miltefosine-treated mice and patients (17, 18). Altogether, involvement of PAF receptor in the miltefosine-induced
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Miltefosine [hexadecylphosphocholine (HPC)] is the only orally bioavailable drug for the disease visceral leishmaniasis, which is caused by the protozoan parasite Leishmania donovani. Although miltefosine has direct leishmanicidal effects, evidence is mounting for its immune system–dependent effects. The mechanism of such indirect antileishmanial effects of miltefosine remains to be discovered. As platelet-activating factor and HPC share structural semblances and both induce killing of intracellular Leishmania, we surmised that platelet-activating factor (PAF) receptor had a significant role in the antileishmanial function of miltefosine. The proposition was supported by molecular dynamic simulation of HPC docking into PAF receptor and by comparison of its leishmanicidal function on PAF receptor–deficient macrophages and mice under HPC treatment. We observed that compared with wild-type macrophages, the PAF receptor–deficient macrophages showed 1) reduced binding of a fluorescent analog of HPC, 2) decreased TNF-a production, and 3) lower miltefosine-induced killing of L. donovani. Miltefosine exhibited significantly compromised leishmanicidal function in PAF receptor–deficient mice. An anti-PAF receptor Ab led to a significant decrease in miltefosine-induced intracellular Leishmania killing and IFN-g production in a macrophage–T cell coculture system. These results indicate significant roles for PAF receptor in the leishmanicidal activity of HPC. The findings open new avenues for a more rational understanding of the mechanism of action of this drug as well as for improved therapeutic strategies. The Journal of Immunology, 2015, 194: 000–000.
2 elimination of intracellular parasites is a novel finding that stresses the dual role of miltefosine as a direct antiparasitic effector and as an immunomodulator that may expand its range of activities on intracellular pathogens.
Materials and Methods Animals, parasite infection, and reagents
to address the binding of miltefosine to PAF receptor–deficient or wildtype (WT) macrophages. Thioglycolate-elicited macrophages from naive WT or PAF receptor–deficient mice were cultured on cover slips in RPMI 1640–10% FCS in six-well tissue culture plates for 24 h. The cover slips with the adherent cells were placed in a perfusion chamber. The perfusion was made using perfusion solution (1% fatty acid–free BSA in PBS) or miltefosine–BODIPY (0.8 mM) in the same perfusion solution. After 1 min, miltefosine–BODIPY was added directly to the imaging chamber via a capillary tube. After 7 min, infusion was restarted to stabilize the fluorescence. The nonbound miltefosine–BODIPY was removed by washing the cells with perfusion solution. All confocal microscopy images were collected with a Zeiss LSM 510 confocal microscope using a 363, 1.4 numerical aperture objective lens with excitation at 488 nm and emission l-max at 526 nm.
Statistical analyses Each experiment was performed at least three times. The in vitro cultures were set in triplicates, and the in vivo experiments were performed with five mice per group. The Student t test was performed to ascertain the significance of the differences between the means of the control and the experimental groups. Alternatively, statistical analysis was performed by ANOVA (two-way ANOVA) followed by the Tukey test.
Results Miltefosine fulfills the conformational requirements to act as a ligand for the PAF receptor As miltefosine and PAF shared a significant structural similarity— both containing a hexadecyl chain and the phosphocholine group (Fig. 1A)—we first modeled miltefosine to examine if it was conformationally similar to PAF. As they exhibited a significant conformational similarity (Fig. 1B), both molecules were docked into the murine PAF receptor (Fig. 1C). During the first 9 ns of the dynamics, the conformation of PAF in the PAF receptor binding cavity becomes quite different from that for miltefosine. Relevant
Macrophage culture and L. donovani infection in vitro Thioglycolate-elicited macrophages (99% pure, as assessed by FACS) from BALB/c or PAF receptor–deficient mice were cultured in 16-well tissue culture slides and infected with promastigotes at a 1:10 macrophage/ promastigote ratio for 12 h. The cultures were incubated for 60 h without treatment. In some cases, 48-h infected cultures were treated with the indicated concentrations of miltefosine for 24 h. The cultures were terminated, fixed with methanol, and stained with Giemsa. The parasites were counted under a light microscope (E600; Nikon, Tokyo, Japan) and expressed as the number of amastigotes per 100 macrophages (12). For comparing miltefosine’s leishmanicidal effects on PAF receptor–deficient and BALB/c macrophages, the cells were infected with promastigotes at a ratio of 1:10 for 4 h. The extracellular promastigotes were washed out, and the cultures were treated with the indicated concentrations of miltefosine for different periods (4 h, 24 h, 72 h) and processed as stated above. Macrophage–T cell coculture. Thioglycolate-elicited BALB/c-derived macrophages were infected with L. donovani promastigotes for 48 h. The uninfected macrophages (UIM) and L. donovani–infected macrophages (IM) were treated with HPC in the presence or absence of anti-PAF receptor Ab for 24 h. The macrophages were washed and pulsed with leishmanial crude soluble Ag (CSA), 10 mg/ml, for 2 h followed by coculture with CD4+ T cells from 10-d L. donovani–infected BALB/c mice. The cells were cultured for 24 h. Confocal microscopy for binding of miltefosine–BODIPY to PAF receptor– deficient and wild-type macrophages. Miltefosine–BODIPY (11-(49,49difluoro-19,39,59,79-tetramethyl-49-bora-39a,49a-diaza-s-indacen-29-yl) undecyl-phosphocholine) was synthesized, as previously described (24). This fluorescent analog of miltefosine showed antiparasitic activity similar to that of miltefosine. This analog was used in confocal time-lapse imaging
FIGURE 1. Structural semblances between miltefosine (HPC) and PAF. (A) Chemical structure: both phosphocholine head and a hexadecyl chain appear in both molecules. (B) Molecular modeling of HPC (upper panel) and PAF (lower panel); hexadecyl chain protruded in a similar manner for both molecules, although PAF is bulkier at the middle owing to its glycerol backbone. (C) MD simulation predicts comparable interactions between PAF receptor and HPC (left panel) or PAF (right panel).
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BALB/c mice were originally obtained from The Jackson Laboratory (Bar Harbor, ME) and subsequently bred in the Institute’s (National Centre for Cell Science) experimental animal facility in individually ventilated cages (Thoren Caging Systems, Hazleton, PA). PAF receptor–deficient mice (19) on the BALB/c background were a kind gift from Prof. Mauro Martins Teixeira (Departamento de Bioquı´mica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil) and were reared under SPF conditions at Instituto de Cieˆncias Biolo´gicas. L. donovani (MHOM/ IN/80/DD8) was maintained in vitro in RPMI 1640 medium, supplemented with 10% FCS (Life Technologies, Grand Island, NY), and virulence was maintained by passage through BALB/c mice, as described earlier (12). Stationary phase promastigotes (2 3 107 per mouse, i.v.) were used for infection of mice. Mice were treated with miltefosine (20 mg/kg body weight) for 7 d consecutively by gavage 2 wk after L. donovani infection. Parasite burden in mice was assessed by limiting dilution and expressed as the negative log of the titer (16). Experimental protocols were approved by the ethical committee CETEA/UFMG protocol 150/09. Miltefosine was purchased from Sigma-Aldrich (St. Louis, MO) and AG Scientific (San Diego, CA). Anti–TNF-a Ab was from BD Biosciences, and anti-PAF receptor Ab was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). PAF receptor–HPC and PAF receptor–PAF interaction by molecular dynamic simulation. The molecular dynamic (MD) simulations were performed using the GROMACS package version 4.5.3 with the GROMOS96 force field (20). The molecular topology file was generated with PRODRG (21). The partial atomic charges were determined using the CHelpG method (22) implemented in the Gaussian09 program with the DFT/ B3LYP/6-311G** basis set. Electrostatic interactions between charged ˚ were calculated explicitly, and long-range electrostatic groups within 9 A interactions were calculated using the particle mesh Ewald method (23) ˚ and a fourth-order spline interpolation. A cutoff with a grid width of 1.2 A ˚ was applied for the Lennard–Jones interactions. Nudistance of 14 A merical integration of the equations of motion used a time step of 2 fs with atomic coordinates saved every 1 ps for further analysis. The molecular docking program AutoDock 4.0 was employed to probe the possible binding sites of the energy-minimum conformations of the PAF receptor derived from the MD simulations.
HPC WORKS THROUGH PAF RECEPTOR
The Journal of Immunology
An anti-PAF receptor Ab inhibits miltefosine-induced leishmanicidal activity in macrophages To verify whether PAF receptor is required for the leishmanicidal activity of miltefosine, the effect of an anti-PAF receptor Ab on parasite elimination and TNF-a production by the macrophage was tested. Both activities (Fig. 2A, 2B) were inhibited by the anti-PAF receptor Ab in a dose-dependent manner. In response to miltefosine, PAF receptor–deficient macrophages failed to enhance the production of TNF-a (Fig. 2C), a cytokine required for miltefosine-induced antileishmanial activity (Fig. 2D), and the anti-PAF receptor Ab reduced the TNF-a–dependent antileishmanial activity of miltefosine (Fig. 2D) in BALB/c macrophages. Whereas the TNF-a–inducing function of HPC was completely inhibited by the anti-PAF receptor Ab (Fig. 2B) and the absence of PAF receptor on macrophages (Fig. 2C), both anti– TNF-a Ab and anti-PAF receptor Ab inhibited the leishmanicidal function of HPC completely (Fig. 2D). Taken together, these observations suggest that PAF receptor–mediated induction of TNF-a is a key factor in the HPC-induced leishmanicidal func-
tion. TNF-a production in response to miltefosine was completely impaired in PAF receptor–deficient macrophages. Although TNF-a was previously shown to be a strong antileishmanial cytokine (25, 26), the involvement of TNF-a in miltefosine-induced PAF receptor–mediated antileishmanial function was never implicated. In this article, our observations report for the first time, to our knowledge, that PAF receptor is involved in miltefosine-induced TNF-a production and subsequent antileishmanial functions. These data suggested that PAF receptor contributed to the antileishmanial activity of miltefosine. The anti-PAF receptor Ab reduces IFN-g production by CD4+ T cells elicited with leishmanial Ags Miltefosine exhibits proinflammatory effects and host-protective antileishmanial functions (12). As the latter was inhibited by PAF receptor blockade, we examined whether this was a consequence of impairment of the miltefosine-induced Th1 functions (12) under these conditions. Therefore, BALB/c-derived macrophages were pulsed with Leishmania CSA and cocultured with CD4+ T cells, isolated from the spleen of BALB/c mice 10 d after L. donovani infection, in the presence of anti-PAF receptor Ab or its control isotype Ab. The anti-PAF receptor Ab significantly reduced miltefosine-induced IFN-g, but increased IL-4, production by leishmanial Ag–specific T cells (Fig. 3A). The parallel culture examining leishmanicidal activity showed that the PAF receptor blockade significantly reduced the leishmanicidal efficacy of HPC (Fig. 3B). These data suggested a significant role for PAF receptor in miltefosine-enhanced T cell IFN-g production, perhaps through the induction of IL-12 (12), which is required for L. donovani elimination. PAF receptor–deficient macrophages showed a significantly impaired miltefosine binding The above-stated results suggested binding of miltefosine to the PAF receptor–deficient macrophages. Therefore, we examined miltefosine–BODIPY binding to WT and PAF receptor–deficient macrophages. The fluorescence associated with PAF receptor– deficient macrophages was significantly lower (,50%) than for
FIGURE 2. Modulation of antileishmanial activity of macrophages by PAF receptor. (A) BALB/c-derived thioglycolate-elicited macrophages were infected with L. donovani promastigotes for 48 h, followed by treatment with miltefosine (HPC) in the presence of the indicated doses of the anti-PAF receptor Ab. An isotype-matched Ab did not have any effect on the antileishmanial effect of HPC (data not plotted). *p , 0.001. (B) TNF-a production, as assessed by ELISA, from the BALB/c-derived thioglycolate-elicited macrophages 24 h after miltefosine treatment was significantly inhibited by the antiPAF receptor Ab in a dose-dependent manner. **p , 0.001. (C) HPC-induced TNF-a production by the thioglycolate-elicited WT (BALB/c) and PAF receptor–deficient macrophages. Thioglycolate-elicited macrophages were infected with L. donovani promastigotes for 4 h. The UIM and infected IM were treated with miltefosine (HPC) for 24 h. (D) BALB/c-derived peritoneal macrophages were infected with L. donovani promastigotes for 48 h, followed by treatment with anti-PAF receptor or anti–TNF-a Ab for 24 h. Following the treatment, the parasite loads in macrophages were calculated and expressed as indicated. Each of these experiments was performed three times. Results from one representative experiment are shown in this figure. Bars stand for means 6 SD. The significance of difference between the means of (A) and (A) + (C) is p , 0.005, whereas the difference between the means of “Control” and (A) + (C) is not significant (p . 0.1). *p , 0.01, **p , 0.001.
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changes were noticed in the dihedral angles of the drugs and in the way miltefosine and PAF receptor interact with PAF receptor. Whereas PAF binding relied mostly on hydrophobic interactions established with the aromatic side chains of W-102, F-107, and W-232, with hydrogen bonds established between their hydroxyl groups of PAF with N-3.79 and W-232 of PAF receptor (Fig. 1C), miltefosine binding is driven by much stronger electrostatic and hydrogen bonding interactions with the protonated H-3.92. The role of H-3.92 in stabilizing the bent miltefosine conformation in the PAF receptor binding cavity was more important, as the N-4.93 at the same position in the PAF-PAF receptor was not involved in any interaction with PAF. In agreement with the conformational similarities and capacity to be recognized by PAF receptor, PAF and miltefosine additionally shared biological activities as well, including their strong antileishmanial activity (12, 14, 15) or p38 MAPK and ERK-1/2 phosphorylation in macrophages (P.R. Gangalum and B. Saha, unpublished observations).
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HPC WORKS THROUGH PAF RECEPTOR
BALB/c macrophages (Fig. 4A–C), stressing the importance of PAF receptor in the binding of miltefosine onto murine macrophages. The residual binding could be due to nonspecific adsorption of miltefosine–BODIPY through its fatty acyl chains to the membrane. PAF receptor deficiency significantly reduced the leishmanicidal function of miltefosine Next, we tested whether the WT and the PAF receptor–deficient macrophages differed in their intrinsic capacities to kill the par-
asite. No significant differences were observed either in the percentage of infected or in the number of amastigotes for untreated WT or PAF receptor–deficient macrophages (Fig. 5). However, the PAF receptor–deficient macrophages showed consistently higher parasite loads than did the WT macrophages after miltefosine treatment (Fig. 5). Miltefosine executed its leishmanicidal function in BALB/c macrophages as early as 4 h post infection, but such activity was significantly compromised in PAF receptor– deficient macrophages. This early antileishmanial function is perhaps mediated through oxidative burst, as this early oxidative
FIGURE 4. Visualization of miltefosine–BODIPY binding at PAF receptor by confocal microscopy. (A) Kinetics of miltefosine–BODIPY binding to macrophages, carried out as described in Materials and Methods. Macrophages from WT and PAF receptor–KO mice on the BALB/c background were used. Measurements were taken every 7 s from 70 macrophages. Graph is a representative experiment from five performed, but the average of two experiments. After 7 min of incubation, we restarted the infusion to wash out the nonbound miltefosine–BODIPY. (B) Peak of the fluorescent intensity of 20 macrophages. Graph is a representative experiment of five performed. White and black bars represent BALB/c and PAF receptor–deficient, respectively. Results are expressed as fluorescence intensity (arbitrary units) and error bars of the fluorescence changes as mean 6SEM. (C) Representative confocal images of miltefosine–BODIPY-bound BALB/c and PAF receptor–deficient macrophages. Control: fluorescence before miltefosine–BODIPY addition. Miltefosine–BODIPY pictures were taken 3 min after miltefosine–BODIPY addition. D.I.C., differential contrast, bright-field aspect of cultures.
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FIGURE 3. Miltefosine-induced Th1 response is reduced by anti-PAF receptor Ab. (A) BALB/c-derived thioglycolate-elicited macrophages were infected with L. donovani promastigotes for 48 h. The UIM and IM were treated with miltefosine (HPC) in the presence or absence of anti-PAF receptor Ab for 24 h. The macrophages were washed and pulsed with leishmanial CSA (10 mg/ml) for 2 h, followed by coculture with CD4+ T cells from 10-d L. donovani–infected BALB/c mice. The cells were cultured for 24 h, and the resulting supernatants were assessed for IL-4 and IFN-g content by ELISA. (B) Under the same experimental conditions, macrophages infected with L. donovani promastigotes in vitro were treated with miltefosine (HPC)(3.2 mM) in the presence or absence of anti-PAF receptor Ab or isotype and cocultured with CD4+ T cells from the BALB/c mice 10 d post infection at a ratio of 1MФ:3T cells. At 72 h later, the parasite load in macrophages, as shown, was enumerated. The experiments were performed three times; one representative set of data is shown. The error bars represent mean 6SD. *p , 0.001.
The Journal of Immunology
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burst was inhibited by a PAF antagonist (P.R. Gangalum, R. Dey, S. Majumdar, and B. Saha, unpublished observations). Nonetheless, these observations substantiated the requirement of PAF receptor expression in the macrophage for full anti-leishmanial activity of miltefosine. PAF receptor deficiency significantly reduces the antileishmanial efficacy of miltefosine in mice Once we substantiated the feasibility of miltefosine as ligand for PAF receptor, we tested whether this was functionally linked to the final outcome of Leishmania infection in a mouse model. To this end, BALB/c mice (WT) and PAF receptor–deficient mice on the BALB/c background were infected with L. donovani followed by miltefosine treatment. The kinetics of the hepatic and splenic parasite burden in untreated BALB/c was comparable to that in PAF receptor–deficient mice (Fig. 6A), but not after miltefosine treatment (20 mg/kg body weight). A substantial reduction of parasite load in the spleen and full clearance of Leishmania from the liver were observed in WT BALB/c mice, but not in PAF receptor–deficient mice (Fig. 6B). A lower dose of miltefosine (10 mg/kg body weight) also diminished parasite burdens in both
mouse strains, but with much lower efficiency in PAF receptor– deficient mice (W. de Castro and L.Q. Vieira, unpublished observations). These data prove the importance of PAF receptor for miltefosine-dependent leishmanicidal activity.
Discussion The antimony-based drugs used for the treatment of VL have toxic side effects. Their prolonged use has led not only to the emergence of antimony-resistant parasites (2) but also to reduced leishmanicidal efficacy (4). For their optimal antileishmanial effects, these drugs require a robust host immune response (4), but L. donovani infection impairs the effective orchestration of the immune response. Therefore, finding less toxic drugs capable of restoring host-protective immune response to the parasite but maintaining an intrinsic activity on the parasite has been a pressing need. The quest for new drugs led to amphotericin B and paromomycin (4), both not orally bioavailable. By contrast, because of its oral bioavailability, miltefosine became the drug of choice for treating VL patients (27). The mechanism of antileishmanial function of miltefosine is not fully known. Reportedly, miltefosine interferes with lipid synthesis
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FIGURE 5. Antileishmanial activity of miltefosine is compromised in PAF receptor–deficient macrophages. Thioglycolate-elicited peritoneal macrophages from BALB/c and PAF receptor–deficient (PAF receptor–KO) mice were obtained 4–5 d after thioglycolate injection. Macrophages were infected with L. donovani promastigotes at a macrophage/parasite ratio of 1:10. The nonphagocytized parasites were washed with PBS, and some cultures were treated with the indicated concentrations of HPC for 4, 24, or 72 h. The cultures were fixed and stained with Giemsa stain, and the percentage of IM and number of amastigotes per IM were enumerated. The cultures were in triplicates. Statistical analysis was performed by two-way ANOVA followed by the Tukey test. The results are representative of three independent experiments performed with similar results. *p , 0.05.
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HPC WORKS THROUGH PAF RECEPTOR
or impairs the bioenergetic homeostasis of the parasite to cause parasite death by an apoptosis-like mechanism (5, 6). Miltefosine mandatorily required interactions with the plasma membrane of the parasite. Driven by its long fatty acyl chain, this drug inserts and accumulates into the phospholipid matrix, leading either to altered membrane dynamics through a detergent-like action (28) or to interference with cellular signaling, similar to the mechanism described in cancer cells (6). Furthermore, miltefosine insertion into the membrane is required for its recognition by the aminophospholipid translocase involved in the translocation of this drug into the cytoplasmic leaflet of the plasma membrane, facilitating its interaction with the intracellular targets (29). The incorporation of miltefosine into the lipid membrane may alter ligand-bound receptor diffusion and signaling intermediate recruitments to the receptor, causing interference with cellular signaling in cancer cells (7, 30). Although these observations supported the direct action of the drug on the parasite, some reports suggest that miltefosine’s immunomodulatory functions (9–12) contribute to the elimination of intracellular amastigotes, although the underlying mechanism for macrophage activation by miltefosine remains unknown. The studies reported in this article indicate that a significant component of the antileishmanial function of miltefosine is executed through PAF receptor. This observation was first supported by the structural semblances between miltefosine and PAF, affording HPC to work as a PAF receptor ligand, a possibility that was further suggested by MD simulation of miltefosine docking to PAF receptor. From a functional perspective, the reduced TNF-a production by BALB/cderived macrophages after the blockade of PAF receptor with an anti-PAF receptor Ab and the inability of PAF receptor–deficient macrophages to synthesize TNF-a in response to miltefosine emphasized miltefosine’s requirement for PAF receptor to exhibit its leishmanicidal functions. The importance of TNF-a in miltefosine-induced antileishmanial effects was stressed, as miltefosine failed to reduce the parasite load in the presence of the
anti–TNF-a Ab. It is quite possible that being a G-protein–coupled receptor, PAF receptor is able to activate the TNF-a converting enzyme, which can cleave membrane-bound pro–TNF-a to release TNF-a (P.R. Gangalum and B. Saha, unpublished observations). Although TNF-a may contribute to inducible NO synthase expression, other works addressing the antileishmanial mechanism of PAF on L. amazonensis do not substantiate a straightforward relationship between NO production driven by PAF and the elimination of Leishmania (13–16). These results lead to an important conclusion that an effective synergy emerges from the dual effects of miltefosine: direct killing of the parasite and restoration of a hostprotective response (31). Miltefosine was active, apparently through the PAF receptor, as early as 4 h after macrophage infection. We observed that 3 h after the addition of miltefosine to L. donovani– infected macrophages (IM), a substantial amount of reactive oxygen species is produced and that production of the reactive oxygen species by these macrophages was inhibited by a PAF antagonist (P.R. Gangalum, R. Dey, S. Majumdar, and B. Saha, unpublished observations). Hence, it is possible that L. donovani is being killed by macrophages exposed to miltefosine by the oxidative burst, triggered by miltefosine via PAF receptor. The involvement of the PAF receptor in triggering this effector mechanism is further corroborated by the fact that this early leishmanicidal activity was not observed in PAF receptor–deficient macrophages. As L. donovani is highly susceptible to reactive oxygen species (32), the observations indicate that the miltefosine-induced oxidative burst is perhaps responsible for its PAF receptor–mediated leishmanicidal function. We also found evidence for a direct effect of miltefosine on parasites inside macrophages at later time points of in vitro infection, when miltefosine promoted parasite death in PAF receptor–knockout (KO) macrophages. Hence, miltefosine would act both via PAF receptor (as early as 4 h post infection of macrophages) or directly on the parasite. IFN-g production by Th1-type CD4+T cells (12) is crippled by Leishmania through reduced IFN-gR expression and enhanced
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FIGURE 6. Antileishmanial activity of miltefosine is compromised in PAF receptor–deficient mice. (A) BALB/c and PAF receptor–KO mice were infected with L donovani as described above. At the indicated times, mice were sacrificed, and quantification of parasites in liver and spleen was performed by limiting dilution. Results are the mean of five mice per time po7int in each group (6SD). Results are of one experiment of two performed with similar results. *Statistical difference of significance. (B) WT BALB/c and PAF receptor–KO mice were infected with L donovani as described in Materials and Methods. At 14 d post infection, daily treatment with vehicle (PBS) or 20 mg/kg body weight of miltefosine (HPC) was initiated and proceeded for 2 wk, when mice were sacrificed. Quantification of parasites was performed by limiting dilution (1:4 serial dilutions) in livers, spleens, and bone marrow. Results are the mean of five mice per time point in each group (mean 6 SD). Results are from one of three experiments performed independently and with similar results. *Statistical difference.
The Journal of Immunology
Disclosures The authors have no financial conflicts of interest.
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Platelet activating factor receptor-deficient mice present delayed interferon-gamma upregulation and high susceptibility to Leishmania amazonensis infection. Microbes Infect. 8: 2569– 2577. 17. Shivahare, R., P. Vishwakarma, N. Parmar, P. K. Yadav, W. Haq, M. Srivastava, S. Gupta, and S. Kar. 2014. Combination of liposomal CpG oligodeoxynucleotide 2006 and miltefosine induces strong cell-mediated immunity during experimental visceral leishmaniasis. PLoS ONE 9: e94596 Available at: http:// journals.plos.org/plosone/article?id=10.1371/journal.pone.0094596. 18. Mukhopadhyay, D., N. K. Das, S. Roy, S. Kundu, J. N. Barbhuiya, and M. Chatterjee. 2011. Miltefosine effectively modulates the cytokine milieu in Indian post kala-azar dermal leishmaniasis. J. Infect. Dis. 204: 1427–1436. 19. Ishii, S., T. Kuwaki, T. Nagase, K. Maki, F. Tashiro, S. Sunaga, W. H. Cao, K. Kume, Y. Fukuchi, K. Ikuta, et al. 1998. Impaired anaphylactic responses with intact sensitivity to endotoxin in mice lacking a platelet-activating factor receptor. J. Exp. Med. 187: 1779–1788. 20. Berendsen, H. J. C., V. D. Spoel, and R. V. Drunen. 1995. GROMACS: a message-passing parallel molecular dynamics implementation. Comput. Phys. Commun. 95: 43–56. 21. van Aalten, D. M., R. Bywater, J. B. Findlay, M. Hendlich, R. W. Hooft, and G. Vriend. 1996. PRODRG, a program for generating molecular topologies and unique molecular descriptors from coordinates of small molecules. J. Comput. Aided Mol. Des. 10: 255–262. 22. Breneman, C. M., and K. B. Wiberg. 1990. Determining atom-centered monopoles from molecular ectrostatic potentials. The need for high sampling density in formamide conformational analysis. J. Comput. Chem. 11: 361–373. 23. Essmannetal, U., L. Perera, M. L. Berkowitz, T. Darden, H. Lee, and L. G. Pedersen. 1995. A smooth particle mesh Ewald method. J. Chem. Phys. 103: 8577–8593. 24. Hornillos, V., E. Carrillo, L. Rivas, F. Amat-Guerri, and A. U. Acun˜a. 2008. Synthesis of BODIPY-labeled alkylphosphocholines with leishmanicidal activity, as fluorescent analogues of miltefosine. Bioorg. Med. Chem. Lett. 18: 6336– 6339. 25. Murray, H. W., A. Jungbluth, E. Ritter, C. Montelibano, and M. W. Marino. 2000. Visceral leishmaniasis in mice devoid of tumor necrosis factor and response to treatment. Infect. Immun. 68: 6289–6293. 26. Tumang, M. C., C. Keogh, L. L. Moldawer, D. C. Helfgott, R. Teitelbaum, J. Hariprashad, and H. W. Murray. 1994. Role and effect of TNF-alpha in experimental visceral leishmaniasis. J. Immunol. 153: 768–775. 27. Croft, S. L., R. A. Neal, W. Pendergast, and J. H. Chan. 1987. The activity of alkyl phosphorylcholines and related derivatives against Leishmania donovani. Biochem. Pharmacol. 36: 2633–2636. 28. Moreira, R. A., S. A. Mendanha, K. S. Fernandes, G. G. Matos, L. Alonso, M. L. Dorta, and A. Alonso. 2014. Miltefosine increases lipid and protein dynamics in Leishmania amazonensis membranes at concentrations similar to those needed for cytotoxicity activity. Antimicrob. Agents Chemother. 58: 3021–3028. 29. Pe´rez-Victoria, F. J., F. Gamarro, M. Ouellette, and S. Castanys. 2003. Functional cloning of the miltefosine transporter. A novel P-type phospholipid translocase from Leishmania involved in drug resistance. J. Biol. Chem. 278: 49965–49971. 30. Arthur, G., and R. Bittman. 1998. The inhibition of cell signaling pathways by antitumor ether lipids. Biochim. Biophys. Acta 1390: 85–102. 31. Saha, B., G. Das, H. Vohra, N. K. Ganguly, and G. C. Mishra. 1995. Macrophage-T cell interaction in experimental visceral leishmaniasis: failure to express costimulatory molecules on Leishmania-infected macrophages and its implication in the suppression of cell-mediated immunity. Eur. J. Immunol. 25: 2492– 2498. 32. Murray, H. W., and C. F. Nathan. 1999. Macrophage microbicidal mechanisms in vivo: reactive nitrogen versus oxygen intermediates in the killing of intracellular visceral Leishmania donovani. J. Exp. Med. 189: 741–746. 33. Bhardwaj, N., L. E. Rosas, W. P. Lafuse, and A. R. Satoskar. 2005. Leishmania inhibits STAT1-mediated IFN-gamma signaling in macrophages: increased tyrosine phosphorylation of dominant negative STAT1beta by Leishmania mexicana. Int. J. Parasitol. 35: 75–82. 34. Goncalves, R., X. Zhang, H. Cohen, A. Debrabant, and D. M. Mosser. 2011. Platelet activation attracts a subpopulation of effector monocytes to sites of Leishmania major infection. J. Exp. Med. 208: 1253–1265.
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STAT-1 inactivation by SHP-1 (12, 33). As a result, a diminished IFN-g responsiveness ensues. As the anti-PAF receptor Ab reduced miltefosine-induced IFN-g production while increasing IL-4 production and parasite growth in a macrophage and T cell coculture setup, we cannot rule out that PAF receptor also plays a role in miltefosine’s antileishmanial function through T cells, as well. It is an intriguing observation, as PAF receptor modulation of T cell response is not reported to date. Therefore, the role of PAF receptor in miltefosine modulation of T cell responses requires an independent and detailed investigation. An alternative explanation for our observations could be an autocrine antileishmanial function of the miltefosine-induced PAF in macrophages. However, as the parasite loads both in untreated macrophages from BALB/c mice and in PAF receptor–deficient mice were comparable, the role of intrinsic PAF in antileishmanial function appears unlikely. If miltefosine, as demonstrated in this study, can act as a ligand to PAF receptor, this may lead to the activation of platelets, cells with a decreased density under progressive leishmaniasis and with high PAF receptor expression. In fact, for L. major, activation of platelets is required for the migration of a specific set of monocytes highly effective in the elimination of Leishmania (34). At the same tune, miltefosinedependent activation of platelets, known to be highly involved in inflammation, may underlie some limitations and contraindications concerning the use of miltefosine in pregnant women. Altogether, our results provide an account of the possible role of PAF receptor in leishmanicidal activity and pathophysiology in miltefosine-treated VL patients. These findings may contribute to a more rational therapy for this disease as well as to improving the design for a new generation of analogs.
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Pallavi R. Gangalum, Waldionê de Castro, Leda Q. Vieira, Ranadhir Dey, Luis Rivas, Shailza Singh, Subrata Majumdar and Bhaskar Saha
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The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 9650 Rockville Pike, Bethesda, MD 20814-3994. Copyright © 2015 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606.
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J Immunol published online 15 May 2015 http://www.jimmunol.org/content/early/2015/05/15/jimmun ol.1401890
Published May 15, 2015, doi:10.4049/jimmunol.1401890 The Journal of Immunology
Platelet-Activating Factor Receptor Contributes to Antileishmanial Function of Miltefosine Pallavi R. Gangalum,*,1 Waldioneˆ de Castro,†,1 Leda Q. Vieira,† Ranadhir Dey,‡ Luis Rivas,x Shailza Singh,* Subrata Majumdar,‡ and Bhaskar Saha*
V
isceral leishmaniasis (VL), a fatal disease with an annual worldwide incidence of 0.5 million new cases (1), is caused by infection with the protozoan species Leishmania infantum, L. chagasi, and L. donovani, the last-named with the highest relevance in terms of mortality and number of patients. Treatment with antimonial drugs has declined rapidly with increased resistance of the parasite. One alternative is liposomal amphotericin B, which requires parenteral administration and is prohibitively expensive (2). So, a new orally bioavailable antileishmanial drug was a pressing need, when miltefosine [hexadecylphosphocholine (HPC)], an alkyl-phosphocholine originally developed as an anticancer drug (3), was proved to be an effective antileishmanial agent in clinics (4). Miltefosine is reported to
*National Centre for Cell Science, Ganeshkhind, Pune 411007, India; †Nucleo de Pesquisa em Cieˆncias Biolo´gicas, Universidade Federal de Ouro Preto, CEP 35400-000 Ouro Preto, Brazil; ‡Bose Institute, Kolkata 700 054, India; and xCentro de Investigaciones Biolo´gicas, Consejo Superior de Investigaciones Cientificas, Unidad Metabolo´ mica, Interacciones y Bioana´lisis, Universidad CEU San Pablo, 28040 Madrid, Spain 1
P.R.G. and W.d.C. share equal contribution to this work.
Received for publication July 24, 2014. Accepted for publication March 30, 2015. This work was supported by partial funding provided by Fundac¸ao de Amparo a` Pesquisa do Estado de Minas Gerais Process CBBAPQ-00082-08; L.R. was supported by Fondo de Investigaciones Sanitarias and VI PN de I+D+I 2008-2011, Instituto de Salud Carlos III PI12-02706, and Subdireccio´n General de Redes y Centros de Investigacio´n Cooperativa-FEDER RD12/0018/0007; P.R.G. and R.D. were Council of Scientific and Industrial Research fellows; W.d.C. and L.Q.V. are Conselho Nacional de Pesquisas fellows. Address correspondence and reprint requests to Dr. Bhaskar Saha, National Centre for Cell Science, Ganeshkhind, Pune 411007, India. E-mail address: sahab@nccs. res.in Abbreviations used in this article: CSA, crude soluble Ag; HPC, hexadecylphosphocholine; IM, infected macrophage; KO, knockout; MD, molecular dynamic; PAF, platelet-activating factor; UIM, uninfected macrophage; VL, visceral leishmaniasis; WT, wild-type. Copyright Ó 2015 by The American Association of Immunologists, Inc. 0022-1767/15/$25.00 www.jimmunol.org/cgi/doi/10.4049/jimmunol.1401890
trigger apoptosis-like effects (5), lipid-dependent cell signaling in cancer cells (6), and impaired phospholipid synthesis in the parasite (7), as well as acting as an immunomodulator, promoting macrophage activation (8–11). Together with our previous report on the host cell signaling–dependent antileishmanial activity of miltefosine (12), these observations imply that miltefosine may work through a cell surface receptor. HPC shared important structural features with platelet-activating factor (PAF), such as a single long fatty acyl chain and a phosphocholine polar head. Although PAF enhanced killing of L. amazonensis, some of its antagonists inhibited the parasite clearance in mouse macrophages (13–15). Accordingly, PAF receptor (PAF receptor) is perhaps the most feasible candidate to serve as an HPC receptor. Indeed, PAF receptor–deficient mice were more susceptible to L. amazonensis infection and showed a delayed IFN-g production and higher arginase activity, suggesting an essential role for PAF receptor signaling in the control of L. amazonensis infection in mice (16). Thus, consistent with its structural similarities to PAF, our hypothesis was that HPC had at least partial requirement for PAF receptor to exert its leishmanicidal function through immunomodulation of macrophages, the obligate host cell for Leishmania. Therefore, we examined whether PAF receptor blockade or PAF receptor deficiency reduced the antileishmanial functions of miltefosine. Our observations showed that an anti-PAF receptor Ab significantly reduced the miltefosine-triggered Th1 response, TNF-a production, and parasite killing in macrophages. In corroboration of these observations, miltefosine-induced L. donovani clearance was significantly impaired in PAF receptor–deficient macrophages and mice. Thus, PAF receptor contributes to miltefosine-induced antileishmanial functions. The PAF receptor–dependent component of HPC perhaps accounts for the heightened inflammation observed in miltefosine-treated mice and patients (17, 18). Altogether, involvement of PAF receptor in the miltefosine-induced
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Miltefosine [hexadecylphosphocholine (HPC)] is the only orally bioavailable drug for the disease visceral leishmaniasis, which is caused by the protozoan parasite Leishmania donovani. Although miltefosine has direct leishmanicidal effects, evidence is mounting for its immune system–dependent effects. The mechanism of such indirect antileishmanial effects of miltefosine remains to be discovered. As platelet-activating factor and HPC share structural semblances and both induce killing of intracellular Leishmania, we surmised that platelet-activating factor (PAF) receptor had a significant role in the antileishmanial function of miltefosine. The proposition was supported by molecular dynamic simulation of HPC docking into PAF receptor and by comparison of its leishmanicidal function on PAF receptor–deficient macrophages and mice under HPC treatment. We observed that compared with wild-type macrophages, the PAF receptor–deficient macrophages showed 1) reduced binding of a fluorescent analog of HPC, 2) decreased TNF-a production, and 3) lower miltefosine-induced killing of L. donovani. Miltefosine exhibited significantly compromised leishmanicidal function in PAF receptor–deficient mice. An anti-PAF receptor Ab led to a significant decrease in miltefosine-induced intracellular Leishmania killing and IFN-g production in a macrophage–T cell coculture system. These results indicate significant roles for PAF receptor in the leishmanicidal activity of HPC. The findings open new avenues for a more rational understanding of the mechanism of action of this drug as well as for improved therapeutic strategies. The Journal of Immunology, 2015, 194: 000–000.
2 elimination of intracellular parasites is a novel finding that stresses the dual role of miltefosine as a direct antiparasitic effector and as an immunomodulator that may expand its range of activities on intracellular pathogens.
Materials and Methods Animals, parasite infection, and reagents
to address the binding of miltefosine to PAF receptor–deficient or wildtype (WT) macrophages. Thioglycolate-elicited macrophages from naive WT or PAF receptor–deficient mice were cultured on cover slips in RPMI 1640–10% FCS in six-well tissue culture plates for 24 h. The cover slips with the adherent cells were placed in a perfusion chamber. The perfusion was made using perfusion solution (1% fatty acid–free BSA in PBS) or miltefosine–BODIPY (0.8 mM) in the same perfusion solution. After 1 min, miltefosine–BODIPY was added directly to the imaging chamber via a capillary tube. After 7 min, infusion was restarted to stabilize the fluorescence. The nonbound miltefosine–BODIPY was removed by washing the cells with perfusion solution. All confocal microscopy images were collected with a Zeiss LSM 510 confocal microscope using a 363, 1.4 numerical aperture objective lens with excitation at 488 nm and emission l-max at 526 nm.
Statistical analyses Each experiment was performed at least three times. The in vitro cultures were set in triplicates, and the in vivo experiments were performed with five mice per group. The Student t test was performed to ascertain the significance of the differences between the means of the control and the experimental groups. Alternatively, statistical analysis was performed by ANOVA (two-way ANOVA) followed by the Tukey test.
Results Miltefosine fulfills the conformational requirements to act as a ligand for the PAF receptor As miltefosine and PAF shared a significant structural similarity— both containing a hexadecyl chain and the phosphocholine group (Fig. 1A)—we first modeled miltefosine to examine if it was conformationally similar to PAF. As they exhibited a significant conformational similarity (Fig. 1B), both molecules were docked into the murine PAF receptor (Fig. 1C). During the first 9 ns of the dynamics, the conformation of PAF in the PAF receptor binding cavity becomes quite different from that for miltefosine. Relevant
Macrophage culture and L. donovani infection in vitro Thioglycolate-elicited macrophages (99% pure, as assessed by FACS) from BALB/c or PAF receptor–deficient mice were cultured in 16-well tissue culture slides and infected with promastigotes at a 1:10 macrophage/ promastigote ratio for 12 h. The cultures were incubated for 60 h without treatment. In some cases, 48-h infected cultures were treated with the indicated concentrations of miltefosine for 24 h. The cultures were terminated, fixed with methanol, and stained with Giemsa. The parasites were counted under a light microscope (E600; Nikon, Tokyo, Japan) and expressed as the number of amastigotes per 100 macrophages (12). For comparing miltefosine’s leishmanicidal effects on PAF receptor–deficient and BALB/c macrophages, the cells were infected with promastigotes at a ratio of 1:10 for 4 h. The extracellular promastigotes were washed out, and the cultures were treated with the indicated concentrations of miltefosine for different periods (4 h, 24 h, 72 h) and processed as stated above. Macrophage–T cell coculture. Thioglycolate-elicited BALB/c-derived macrophages were infected with L. donovani promastigotes for 48 h. The uninfected macrophages (UIM) and L. donovani–infected macrophages (IM) were treated with HPC in the presence or absence of anti-PAF receptor Ab for 24 h. The macrophages were washed and pulsed with leishmanial crude soluble Ag (CSA), 10 mg/ml, for 2 h followed by coculture with CD4+ T cells from 10-d L. donovani–infected BALB/c mice. The cells were cultured for 24 h. Confocal microscopy for binding of miltefosine–BODIPY to PAF receptor– deficient and wild-type macrophages. Miltefosine–BODIPY (11-(49,49difluoro-19,39,59,79-tetramethyl-49-bora-39a,49a-diaza-s-indacen-29-yl) undecyl-phosphocholine) was synthesized, as previously described (24). This fluorescent analog of miltefosine showed antiparasitic activity similar to that of miltefosine. This analog was used in confocal time-lapse imaging
FIGURE 1. Structural semblances between miltefosine (HPC) and PAF. (A) Chemical structure: both phosphocholine head and a hexadecyl chain appear in both molecules. (B) Molecular modeling of HPC (upper panel) and PAF (lower panel); hexadecyl chain protruded in a similar manner for both molecules, although PAF is bulkier at the middle owing to its glycerol backbone. (C) MD simulation predicts comparable interactions between PAF receptor and HPC (left panel) or PAF (right panel).
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BALB/c mice were originally obtained from The Jackson Laboratory (Bar Harbor, ME) and subsequently bred in the Institute’s (National Centre for Cell Science) experimental animal facility in individually ventilated cages (Thoren Caging Systems, Hazleton, PA). PAF receptor–deficient mice (19) on the BALB/c background were a kind gift from Prof. Mauro Martins Teixeira (Departamento de Bioquı´mica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil) and were reared under SPF conditions at Instituto de Cieˆncias Biolo´gicas. L. donovani (MHOM/ IN/80/DD8) was maintained in vitro in RPMI 1640 medium, supplemented with 10% FCS (Life Technologies, Grand Island, NY), and virulence was maintained by passage through BALB/c mice, as described earlier (12). Stationary phase promastigotes (2 3 107 per mouse, i.v.) were used for infection of mice. Mice were treated with miltefosine (20 mg/kg body weight) for 7 d consecutively by gavage 2 wk after L. donovani infection. Parasite burden in mice was assessed by limiting dilution and expressed as the negative log of the titer (16). Experimental protocols were approved by the ethical committee CETEA/UFMG protocol 150/09. Miltefosine was purchased from Sigma-Aldrich (St. Louis, MO) and AG Scientific (San Diego, CA). Anti–TNF-a Ab was from BD Biosciences, and anti-PAF receptor Ab was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). PAF receptor–HPC and PAF receptor–PAF interaction by molecular dynamic simulation. The molecular dynamic (MD) simulations were performed using the GROMACS package version 4.5.3 with the GROMOS96 force field (20). The molecular topology file was generated with PRODRG (21). The partial atomic charges were determined using the CHelpG method (22) implemented in the Gaussian09 program with the DFT/ B3LYP/6-311G** basis set. Electrostatic interactions between charged ˚ were calculated explicitly, and long-range electrostatic groups within 9 A interactions were calculated using the particle mesh Ewald method (23) ˚ and a fourth-order spline interpolation. A cutoff with a grid width of 1.2 A ˚ was applied for the Lennard–Jones interactions. Nudistance of 14 A merical integration of the equations of motion used a time step of 2 fs with atomic coordinates saved every 1 ps for further analysis. The molecular docking program AutoDock 4.0 was employed to probe the possible binding sites of the energy-minimum conformations of the PAF receptor derived from the MD simulations.
HPC WORKS THROUGH PAF RECEPTOR
The Journal of Immunology
An anti-PAF receptor Ab inhibits miltefosine-induced leishmanicidal activity in macrophages To verify whether PAF receptor is required for the leishmanicidal activity of miltefosine, the effect of an anti-PAF receptor Ab on parasite elimination and TNF-a production by the macrophage was tested. Both activities (Fig. 2A, 2B) were inhibited by the anti-PAF receptor Ab in a dose-dependent manner. In response to miltefosine, PAF receptor–deficient macrophages failed to enhance the production of TNF-a (Fig. 2C), a cytokine required for miltefosine-induced antileishmanial activity (Fig. 2D), and the anti-PAF receptor Ab reduced the TNF-a–dependent antileishmanial activity of miltefosine (Fig. 2D) in BALB/c macrophages. Whereas the TNF-a–inducing function of HPC was completely inhibited by the anti-PAF receptor Ab (Fig. 2B) and the absence of PAF receptor on macrophages (Fig. 2C), both anti– TNF-a Ab and anti-PAF receptor Ab inhibited the leishmanicidal function of HPC completely (Fig. 2D). Taken together, these observations suggest that PAF receptor–mediated induction of TNF-a is a key factor in the HPC-induced leishmanicidal func-
tion. TNF-a production in response to miltefosine was completely impaired in PAF receptor–deficient macrophages. Although TNF-a was previously shown to be a strong antileishmanial cytokine (25, 26), the involvement of TNF-a in miltefosine-induced PAF receptor–mediated antileishmanial function was never implicated. In this article, our observations report for the first time, to our knowledge, that PAF receptor is involved in miltefosine-induced TNF-a production and subsequent antileishmanial functions. These data suggested that PAF receptor contributed to the antileishmanial activity of miltefosine. The anti-PAF receptor Ab reduces IFN-g production by CD4+ T cells elicited with leishmanial Ags Miltefosine exhibits proinflammatory effects and host-protective antileishmanial functions (12). As the latter was inhibited by PAF receptor blockade, we examined whether this was a consequence of impairment of the miltefosine-induced Th1 functions (12) under these conditions. Therefore, BALB/c-derived macrophages were pulsed with Leishmania CSA and cocultured with CD4+ T cells, isolated from the spleen of BALB/c mice 10 d after L. donovani infection, in the presence of anti-PAF receptor Ab or its control isotype Ab. The anti-PAF receptor Ab significantly reduced miltefosine-induced IFN-g, but increased IL-4, production by leishmanial Ag–specific T cells (Fig. 3A). The parallel culture examining leishmanicidal activity showed that the PAF receptor blockade significantly reduced the leishmanicidal efficacy of HPC (Fig. 3B). These data suggested a significant role for PAF receptor in miltefosine-enhanced T cell IFN-g production, perhaps through the induction of IL-12 (12), which is required for L. donovani elimination. PAF receptor–deficient macrophages showed a significantly impaired miltefosine binding The above-stated results suggested binding of miltefosine to the PAF receptor–deficient macrophages. Therefore, we examined miltefosine–BODIPY binding to WT and PAF receptor–deficient macrophages. The fluorescence associated with PAF receptor– deficient macrophages was significantly lower (,50%) than for
FIGURE 2. Modulation of antileishmanial activity of macrophages by PAF receptor. (A) BALB/c-derived thioglycolate-elicited macrophages were infected with L. donovani promastigotes for 48 h, followed by treatment with miltefosine (HPC) in the presence of the indicated doses of the anti-PAF receptor Ab. An isotype-matched Ab did not have any effect on the antileishmanial effect of HPC (data not plotted). *p , 0.001. (B) TNF-a production, as assessed by ELISA, from the BALB/c-derived thioglycolate-elicited macrophages 24 h after miltefosine treatment was significantly inhibited by the antiPAF receptor Ab in a dose-dependent manner. **p , 0.001. (C) HPC-induced TNF-a production by the thioglycolate-elicited WT (BALB/c) and PAF receptor–deficient macrophages. Thioglycolate-elicited macrophages were infected with L. donovani promastigotes for 4 h. The UIM and infected IM were treated with miltefosine (HPC) for 24 h. (D) BALB/c-derived peritoneal macrophages were infected with L. donovani promastigotes for 48 h, followed by treatment with anti-PAF receptor or anti–TNF-a Ab for 24 h. Following the treatment, the parasite loads in macrophages were calculated and expressed as indicated. Each of these experiments was performed three times. Results from one representative experiment are shown in this figure. Bars stand for means 6 SD. The significance of difference between the means of (A) and (A) + (C) is p , 0.005, whereas the difference between the means of “Control” and (A) + (C) is not significant (p . 0.1). *p , 0.01, **p , 0.001.
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changes were noticed in the dihedral angles of the drugs and in the way miltefosine and PAF receptor interact with PAF receptor. Whereas PAF binding relied mostly on hydrophobic interactions established with the aromatic side chains of W-102, F-107, and W-232, with hydrogen bonds established between their hydroxyl groups of PAF with N-3.79 and W-232 of PAF receptor (Fig. 1C), miltefosine binding is driven by much stronger electrostatic and hydrogen bonding interactions with the protonated H-3.92. The role of H-3.92 in stabilizing the bent miltefosine conformation in the PAF receptor binding cavity was more important, as the N-4.93 at the same position in the PAF-PAF receptor was not involved in any interaction with PAF. In agreement with the conformational similarities and capacity to be recognized by PAF receptor, PAF and miltefosine additionally shared biological activities as well, including their strong antileishmanial activity (12, 14, 15) or p38 MAPK and ERK-1/2 phosphorylation in macrophages (P.R. Gangalum and B. Saha, unpublished observations).
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HPC WORKS THROUGH PAF RECEPTOR
BALB/c macrophages (Fig. 4A–C), stressing the importance of PAF receptor in the binding of miltefosine onto murine macrophages. The residual binding could be due to nonspecific adsorption of miltefosine–BODIPY through its fatty acyl chains to the membrane. PAF receptor deficiency significantly reduced the leishmanicidal function of miltefosine Next, we tested whether the WT and the PAF receptor–deficient macrophages differed in their intrinsic capacities to kill the par-
asite. No significant differences were observed either in the percentage of infected or in the number of amastigotes for untreated WT or PAF receptor–deficient macrophages (Fig. 5). However, the PAF receptor–deficient macrophages showed consistently higher parasite loads than did the WT macrophages after miltefosine treatment (Fig. 5). Miltefosine executed its leishmanicidal function in BALB/c macrophages as early as 4 h post infection, but such activity was significantly compromised in PAF receptor– deficient macrophages. This early antileishmanial function is perhaps mediated through oxidative burst, as this early oxidative
FIGURE 4. Visualization of miltefosine–BODIPY binding at PAF receptor by confocal microscopy. (A) Kinetics of miltefosine–BODIPY binding to macrophages, carried out as described in Materials and Methods. Macrophages from WT and PAF receptor–KO mice on the BALB/c background were used. Measurements were taken every 7 s from 70 macrophages. Graph is a representative experiment from five performed, but the average of two experiments. After 7 min of incubation, we restarted the infusion to wash out the nonbound miltefosine–BODIPY. (B) Peak of the fluorescent intensity of 20 macrophages. Graph is a representative experiment of five performed. White and black bars represent BALB/c and PAF receptor–deficient, respectively. Results are expressed as fluorescence intensity (arbitrary units) and error bars of the fluorescence changes as mean 6SEM. (C) Representative confocal images of miltefosine–BODIPY-bound BALB/c and PAF receptor–deficient macrophages. Control: fluorescence before miltefosine–BODIPY addition. Miltefosine–BODIPY pictures were taken 3 min after miltefosine–BODIPY addition. D.I.C., differential contrast, bright-field aspect of cultures.
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FIGURE 3. Miltefosine-induced Th1 response is reduced by anti-PAF receptor Ab. (A) BALB/c-derived thioglycolate-elicited macrophages were infected with L. donovani promastigotes for 48 h. The UIM and IM were treated with miltefosine (HPC) in the presence or absence of anti-PAF receptor Ab for 24 h. The macrophages were washed and pulsed with leishmanial CSA (10 mg/ml) for 2 h, followed by coculture with CD4+ T cells from 10-d L. donovani–infected BALB/c mice. The cells were cultured for 24 h, and the resulting supernatants were assessed for IL-4 and IFN-g content by ELISA. (B) Under the same experimental conditions, macrophages infected with L. donovani promastigotes in vitro were treated with miltefosine (HPC)(3.2 mM) in the presence or absence of anti-PAF receptor Ab or isotype and cocultured with CD4+ T cells from the BALB/c mice 10 d post infection at a ratio of 1MФ:3T cells. At 72 h later, the parasite load in macrophages, as shown, was enumerated. The experiments were performed three times; one representative set of data is shown. The error bars represent mean 6SD. *p , 0.001.
The Journal of Immunology
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burst was inhibited by a PAF antagonist (P.R. Gangalum, R. Dey, S. Majumdar, and B. Saha, unpublished observations). Nonetheless, these observations substantiated the requirement of PAF receptor expression in the macrophage for full anti-leishmanial activity of miltefosine. PAF receptor deficiency significantly reduces the antileishmanial efficacy of miltefosine in mice Once we substantiated the feasibility of miltefosine as ligand for PAF receptor, we tested whether this was functionally linked to the final outcome of Leishmania infection in a mouse model. To this end, BALB/c mice (WT) and PAF receptor–deficient mice on the BALB/c background were infected with L. donovani followed by miltefosine treatment. The kinetics of the hepatic and splenic parasite burden in untreated BALB/c was comparable to that in PAF receptor–deficient mice (Fig. 6A), but not after miltefosine treatment (20 mg/kg body weight). A substantial reduction of parasite load in the spleen and full clearance of Leishmania from the liver were observed in WT BALB/c mice, but not in PAF receptor–deficient mice (Fig. 6B). A lower dose of miltefosine (10 mg/kg body weight) also diminished parasite burdens in both
mouse strains, but with much lower efficiency in PAF receptor– deficient mice (W. de Castro and L.Q. Vieira, unpublished observations). These data prove the importance of PAF receptor for miltefosine-dependent leishmanicidal activity.
Discussion The antimony-based drugs used for the treatment of VL have toxic side effects. Their prolonged use has led not only to the emergence of antimony-resistant parasites (2) but also to reduced leishmanicidal efficacy (4). For their optimal antileishmanial effects, these drugs require a robust host immune response (4), but L. donovani infection impairs the effective orchestration of the immune response. Therefore, finding less toxic drugs capable of restoring host-protective immune response to the parasite but maintaining an intrinsic activity on the parasite has been a pressing need. The quest for new drugs led to amphotericin B and paromomycin (4), both not orally bioavailable. By contrast, because of its oral bioavailability, miltefosine became the drug of choice for treating VL patients (27). The mechanism of antileishmanial function of miltefosine is not fully known. Reportedly, miltefosine interferes with lipid synthesis
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FIGURE 5. Antileishmanial activity of miltefosine is compromised in PAF receptor–deficient macrophages. Thioglycolate-elicited peritoneal macrophages from BALB/c and PAF receptor–deficient (PAF receptor–KO) mice were obtained 4–5 d after thioglycolate injection. Macrophages were infected with L. donovani promastigotes at a macrophage/parasite ratio of 1:10. The nonphagocytized parasites were washed with PBS, and some cultures were treated with the indicated concentrations of HPC for 4, 24, or 72 h. The cultures were fixed and stained with Giemsa stain, and the percentage of IM and number of amastigotes per IM were enumerated. The cultures were in triplicates. Statistical analysis was performed by two-way ANOVA followed by the Tukey test. The results are representative of three independent experiments performed with similar results. *p , 0.05.
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HPC WORKS THROUGH PAF RECEPTOR
or impairs the bioenergetic homeostasis of the parasite to cause parasite death by an apoptosis-like mechanism (5, 6). Miltefosine mandatorily required interactions with the plasma membrane of the parasite. Driven by its long fatty acyl chain, this drug inserts and accumulates into the phospholipid matrix, leading either to altered membrane dynamics through a detergent-like action (28) or to interference with cellular signaling, similar to the mechanism described in cancer cells (6). Furthermore, miltefosine insertion into the membrane is required for its recognition by the aminophospholipid translocase involved in the translocation of this drug into the cytoplasmic leaflet of the plasma membrane, facilitating its interaction with the intracellular targets (29). The incorporation of miltefosine into the lipid membrane may alter ligand-bound receptor diffusion and signaling intermediate recruitments to the receptor, causing interference with cellular signaling in cancer cells (7, 30). Although these observations supported the direct action of the drug on the parasite, some reports suggest that miltefosine’s immunomodulatory functions (9–12) contribute to the elimination of intracellular amastigotes, although the underlying mechanism for macrophage activation by miltefosine remains unknown. The studies reported in this article indicate that a significant component of the antileishmanial function of miltefosine is executed through PAF receptor. This observation was first supported by the structural semblances between miltefosine and PAF, affording HPC to work as a PAF receptor ligand, a possibility that was further suggested by MD simulation of miltefosine docking to PAF receptor. From a functional perspective, the reduced TNF-a production by BALB/cderived macrophages after the blockade of PAF receptor with an anti-PAF receptor Ab and the inability of PAF receptor–deficient macrophages to synthesize TNF-a in response to miltefosine emphasized miltefosine’s requirement for PAF receptor to exhibit its leishmanicidal functions. The importance of TNF-a in miltefosine-induced antileishmanial effects was stressed, as miltefosine failed to reduce the parasite load in the presence of the
anti–TNF-a Ab. It is quite possible that being a G-protein–coupled receptor, PAF receptor is able to activate the TNF-a converting enzyme, which can cleave membrane-bound pro–TNF-a to release TNF-a (P.R. Gangalum and B. Saha, unpublished observations). Although TNF-a may contribute to inducible NO synthase expression, other works addressing the antileishmanial mechanism of PAF on L. amazonensis do not substantiate a straightforward relationship between NO production driven by PAF and the elimination of Leishmania (13–16). These results lead to an important conclusion that an effective synergy emerges from the dual effects of miltefosine: direct killing of the parasite and restoration of a hostprotective response (31). Miltefosine was active, apparently through the PAF receptor, as early as 4 h after macrophage infection. We observed that 3 h after the addition of miltefosine to L. donovani– infected macrophages (IM), a substantial amount of reactive oxygen species is produced and that production of the reactive oxygen species by these macrophages was inhibited by a PAF antagonist (P.R. Gangalum, R. Dey, S. Majumdar, and B. Saha, unpublished observations). Hence, it is possible that L. donovani is being killed by macrophages exposed to miltefosine by the oxidative burst, triggered by miltefosine via PAF receptor. The involvement of the PAF receptor in triggering this effector mechanism is further corroborated by the fact that this early leishmanicidal activity was not observed in PAF receptor–deficient macrophages. As L. donovani is highly susceptible to reactive oxygen species (32), the observations indicate that the miltefosine-induced oxidative burst is perhaps responsible for its PAF receptor–mediated leishmanicidal function. We also found evidence for a direct effect of miltefosine on parasites inside macrophages at later time points of in vitro infection, when miltefosine promoted parasite death in PAF receptor–knockout (KO) macrophages. Hence, miltefosine would act both via PAF receptor (as early as 4 h post infection of macrophages) or directly on the parasite. IFN-g production by Th1-type CD4+T cells (12) is crippled by Leishmania through reduced IFN-gR expression and enhanced
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FIGURE 6. Antileishmanial activity of miltefosine is compromised in PAF receptor–deficient mice. (A) BALB/c and PAF receptor–KO mice were infected with L donovani as described above. At the indicated times, mice were sacrificed, and quantification of parasites in liver and spleen was performed by limiting dilution. Results are the mean of five mice per time po7int in each group (6SD). Results are of one experiment of two performed with similar results. *Statistical difference of significance. (B) WT BALB/c and PAF receptor–KO mice were infected with L donovani as described in Materials and Methods. At 14 d post infection, daily treatment with vehicle (PBS) or 20 mg/kg body weight of miltefosine (HPC) was initiated and proceeded for 2 wk, when mice were sacrificed. Quantification of parasites was performed by limiting dilution (1:4 serial dilutions) in livers, spleens, and bone marrow. Results are the mean of five mice per time point in each group (mean 6 SD). Results are from one of three experiments performed independently and with similar results. *Statistical difference.
The Journal of Immunology
Disclosures The authors have no financial conflicts of interest.
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STAT-1 inactivation by SHP-1 (12, 33). As a result, a diminished IFN-g responsiveness ensues. As the anti-PAF receptor Ab reduced miltefosine-induced IFN-g production while increasing IL-4 production and parasite growth in a macrophage and T cell coculture setup, we cannot rule out that PAF receptor also plays a role in miltefosine’s antileishmanial function through T cells, as well. It is an intriguing observation, as PAF receptor modulation of T cell response is not reported to date. Therefore, the role of PAF receptor in miltefosine modulation of T cell responses requires an independent and detailed investigation. An alternative explanation for our observations could be an autocrine antileishmanial function of the miltefosine-induced PAF in macrophages. However, as the parasite loads both in untreated macrophages from BALB/c mice and in PAF receptor–deficient mice were comparable, the role of intrinsic PAF in antileishmanial function appears unlikely. If miltefosine, as demonstrated in this study, can act as a ligand to PAF receptor, this may lead to the activation of platelets, cells with a decreased density under progressive leishmaniasis and with high PAF receptor expression. In fact, for L. major, activation of platelets is required for the migration of a specific set of monocytes highly effective in the elimination of Leishmania (34). At the same tune, miltefosinedependent activation of platelets, known to be highly involved in inflammation, may underlie some limitations and contraindications concerning the use of miltefosine in pregnant women. Altogether, our results provide an account of the possible role of PAF receptor in leishmanicidal activity and pathophysiology in miltefosine-treated VL patients. These findings may contribute to a more rational therapy for this disease as well as to improving the design for a new generation of analogs.
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