72
Poster Presentations / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 3 (2013) 67–99
PP012. Table 1. Umbilical cord morphology. PE (n = 31)
FGR (n = 26)
PTB (n = 24)
Controls (n = 24)
Overall P-value
43.25⁄
50.09
46.08
50.94
P = 0.006
(19–75)
(27–69)
(20–64)
(24–86)
74.06⁄
68.65⁄
85.88⁄
112.63
(37.84–131.21)
(31.87–145.35)
(55.80–109.69)
(64.89–182.60)
4.39⁄
5.55
6.80
7.20
(1.66–17.43)
(1.63–10.45)
(2.36–13.69)
(4.27–13.12)
2.29⁄
2.52⁄
3.11
3.46
(1.62–3.44)
(1.62–3.79)
(1.93–5.69)
(2.45–5.26)
7.18⁄
7.32⁄
7.53
8.99
(2.88–13.85)
(4.16–12.37)
(4.85–16.23)
(5.59–17.41)
Macroscopy Length (cm)
Microscopy Total cord area (mm2) Vein wall area (mm2) Artery wall area (mm2) Vein elastic fibres (%)
P < 0.001 P = 0.020 P < 0.001 P = 0.022
ANOVA, mean (range). ⁄ P < 0.05 compared to controls.
Conclusion: Our study demonstrates umbilical cord vascular abnormalities in PE, FGR and PTB, suggesting early vascular damage. Future studies should focus on similar structural changes in foetal vasculature and associations with cardiovascular disease in later life. doi:10.1016/j.preghy.2013.04.040
PP013. Single nucleotide polymorphisms of the maternal cystathionine-b-synthase gene are associated with preeclampsia (PE) Holwerda Kim, Weedon-Fekjær Susanne, Staff Annetine, Nolte Ilja, Goor Harry van, Lely Titia, Faas Marijke Introduction: Cystathionine-b-synthase (CBS) produces the vasodilatory and anti-inflammatory hydrogen sulfide (H2S) by conversion of homocysteine (Hcy). H2S is involved in placental vascular tone regulation. Previously, we showed that woman with PE have hyperhomocysteinemia and decreased placental CBS gene expression. Objectives: Aberrant CBS gene expression may play a role in hyperhomocysteinemia and decreased H2S and could be involved in pathogenesis of PE. We studied whether the presence of CBS single nucleotide polymorphisms (SNPs) is associated with the development of PE. Methods: Six CBS SNPs (rs12329764, rs2851391, rs234713, rs234706, rs1789953, rs11203172) were genotyped in 99 controls, 60 severe, and 39 mild PE cases. Severe and mild PE cases were additionally subdivided into late(>34 weeks) and early-onset (
Poster Presentations / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 3 (2013) 67–99
PP012. Table 1. Umbilical cord morphology. PE (n = 31)
FGR (n = 26)
PTB (n = 24)
Controls (n = 24)
Overall P-value
43.25⁄
50.09
46.08
50.94
P = 0.006
(19–75)
(27–69)
(20–64)
(24–86)
74.06⁄
68.65⁄
85.88⁄
112.63
(37.84–131.21)
(31.87–145.35)
(55.80–109.69)
(64.89–182.60)
4.39⁄
5.55
6.80
7.20
(1.66–17.43)
(1.63–10.45)
(2.36–13.69)
(4.27–13.12)
2.29⁄
2.52⁄
3.11
3.46
(1.62–3.44)
(1.62–3.79)
(1.93–5.69)
(2.45–5.26)
7.18⁄
7.32⁄
7.53
8.99
(2.88–13.85)
(4.16–12.37)
(4.85–16.23)
(5.59–17.41)
Macroscopy Length (cm)
Microscopy Total cord area (mm2) Vein wall area (mm2) Artery wall area (mm2) Vein elastic fibres (%)
P < 0.001 P = 0.020 P < 0.001 P = 0.022
ANOVA, mean (range). ⁄ P < 0.05 compared to controls.
Conclusion: Our study demonstrates umbilical cord vascular abnormalities in PE, FGR and PTB, suggesting early vascular damage. Future studies should focus on similar structural changes in foetal vasculature and associations with cardiovascular disease in later life. doi:10.1016/j.preghy.2013.04.040
PP013. Single nucleotide polymorphisms of the maternal cystathionine-b-synthase gene are associated with preeclampsia (PE) Holwerda Kim, Weedon-Fekjær Susanne, Staff Annetine, Nolte Ilja, Goor Harry van, Lely Titia, Faas Marijke Introduction: Cystathionine-b-synthase (CBS) produces the vasodilatory and anti-inflammatory hydrogen sulfide (H2S) by conversion of homocysteine (Hcy). H2S is involved in placental vascular tone regulation. Previously, we showed that woman with PE have hyperhomocysteinemia and decreased placental CBS gene expression. Objectives: Aberrant CBS gene expression may play a role in hyperhomocysteinemia and decreased H2S and could be involved in pathogenesis of PE. We studied whether the presence of CBS single nucleotide polymorphisms (SNPs) is associated with the development of PE. Methods: Six CBS SNPs (rs12329764, rs2851391, rs234713, rs234706, rs1789953, rs11203172) were genotyped in 99 controls, 60 severe, and 39 mild PE cases. Severe and mild PE cases were additionally subdivided into late(>34 weeks) and early-onset (
