704667 research-article2017
BBI0010.1177/1177932217704667Bioinformatics and Biology InsightsAgrawal et al
Prepartal Energy Intake Alters Blood Polymorphonuclear Leukocyte Transcriptome During the Peripartal Period in Holstein Cows
Bioinformatics and Biology Insights Volume 11: 1–17 © The Author(s) 2017 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav DOI: 10.1177/1177932217704667 https://doi.org/10.1177/1177932217704667
A Agrawal1, MJ Khan1, DE Graugnard1, M Vailati-Riboni1, SL Rodriguez-Zas1, JS Osorio2 and JJ Loor1 1Division
of Nutritional Sciences, Department of Animal Sciences, University of Illinois at Urbana–Champaign, Urbana, IL, USA. 2Department of Dairy Science, South Dakota State University, Brookings, SD, USA.
ABSTRACT: In the dairy industry, cow health and farmer profits depend on the balance between diet (ie, nutrient composition, daily intake) and metabolism. This is especially true during the transition period, where dramatic physiological changes foster vulnerability to immunosuppression, negative energy balance, and clinical and subclinical disorders. Using an Agilent microarray platform, this study examined changes in the transcriptome of bovine polymorphonuclear leukocytes (PMNLs) due to prepartal dietary intake. Holstein cows were fed a highstraw, control-energy diet (CON; NEL = 1.34 Mcal/kg) or overfed a moderate-energy diet (OVE; NEL = 1.62 Mcal/kg) during the dry period. Blood for PMNL isolation and metabolite analysis was collected at −14 and +7 days relative to parturition. At an analysis of variance false discovery rate
BBI0010.1177/1177932217704667Bioinformatics and Biology InsightsAgrawal et al
Prepartal Energy Intake Alters Blood Polymorphonuclear Leukocyte Transcriptome During the Peripartal Period in Holstein Cows
Bioinformatics and Biology Insights Volume 11: 1–17 © The Author(s) 2017 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav DOI: 10.1177/1177932217704667 https://doi.org/10.1177/1177932217704667
A Agrawal1, MJ Khan1, DE Graugnard1, M Vailati-Riboni1, SL Rodriguez-Zas1, JS Osorio2 and JJ Loor1 1Division
of Nutritional Sciences, Department of Animal Sciences, University of Illinois at Urbana–Champaign, Urbana, IL, USA. 2Department of Dairy Science, South Dakota State University, Brookings, SD, USA.
ABSTRACT: In the dairy industry, cow health and farmer profits depend on the balance between diet (ie, nutrient composition, daily intake) and metabolism. This is especially true during the transition period, where dramatic physiological changes foster vulnerability to immunosuppression, negative energy balance, and clinical and subclinical disorders. Using an Agilent microarray platform, this study examined changes in the transcriptome of bovine polymorphonuclear leukocytes (PMNLs) due to prepartal dietary intake. Holstein cows were fed a highstraw, control-energy diet (CON; NEL = 1.34 Mcal/kg) or overfed a moderate-energy diet (OVE; NEL = 1.62 Mcal/kg) during the dry period. Blood for PMNL isolation and metabolite analysis was collected at −14 and +7 days relative to parturition. At an analysis of variance false discovery rate
