1298 Nucleic Acids Research, Vol. 18, No. 5

© Oxford University Press 1990

Primary structure of cDNA sequence

mouse

chromogranin B deduced from

Christian G.Linard, Majambu Mbikay, Nabil G.Seidah1 and Michel Chretien* J.A. de Seve Laboratories of Molecular and 1Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada Submitted January 24, 1990

EMBL accession no. X51429

The primary structure of mouse chromogranin B (CGB) was deduced from the sequence of cDNAs obtained after screening a pituitary cDNA library with synthetic oligonucleotides corresponding to nucleotides 173 to 288 of human CGB (1). Mouse pre-CGB is 677 aa long and begins with a 20-residue putative signal peptide; it contains two Cysteine residues (arrows) at positions + 16 and +37, 18 pairs of basic residues (asterisks), a Tyrosine sulfate site (open circle) and no potential Nglycosylation site. Between human and mouse CGBs, there is an overall identity of 70% in nucleotide sequence and of 61 % in amino acid sequence (bold characters); but regions of higher conservation are observed around the sulfatable Tyrosine (underlined), at the amino-terminal (aa -20 to +72: 77%) and at the carboxyl terminal (aa 491 to 657: 81 %).

ACKNOWLEDGEMENT Funded by MRC Canada. REFERENCE 1. Benedum,U.M., Lamouroux,A., Konecki,D.S., Rosa,P., Hille,A., Baeuerle,P.A., Frank,R., Lottspeich,F., Mallet,J. and Huttner,W.B. (1987) EMBO J. 5, 1203-1211.

M Q P A M L L G L L G A A A L A A V S I A P V GGCGGACGCGCACCCGCCGAGCTCCTCCTACACTCAGCTGGCGAGAGGCACCATGCAGCC GGCTATGCTCCTCGGCCTCCTGGGCGCAGCGGCTCTGGCCGCTGTCAGCTCAGCTCCAGT

120

K SB D N R D H N E E M V P T I T P K C R Q V L K K V T R C II K V L S N A L B GGATAACAGGGATCACAATGAAGAAATGGTGACTCGCTGCATTATCGAAGTCCTCTCAAA TGCCCTATCCAAGTCCAGTGTTCCAACGATCACCCCTGAGTGCCGGCAAGTCCTGAAGAA

44 240

S G K E V K G E E K G E N Q N S K F E V R L L R D P A D A S G T R W A S S R E D GAGTGGAAAAGAGGTCAAAGGTGAAGAGAAAGGTGAAAACCAGAACTCGAAGTTTGAAGT ACGGTTGCTAAGAGACCCAGCCGATGCCTCAGGAACCCGCTGGGCCTCCAGTAGGGAGGA

84 360

NW T E G G G H S R E G V D D Q E S L R P S N A G A P V E D S Q G Q T K V G N E TGCAGGCGCTCCAGTAGAAGACTCTCAAGGCCAGACGAAGGTAGGCAATGAGAAGTGGAC AGAAGGAGGAGGACACAGCCGAGAGGGAGTGGATGATCAAGAGAGTCTCCGTCCCTCCAA

480

Q Q A S K E A K I Y H S E E R V G K E R E E E G K I Y P M G E H R E D A G E E CCAACAAGCGTCCAAAGAAGCAAAGATATACCATTCGGAAGAGAGGGTGGGAAAAGAGAG GGAGAAAGAAGAAGGCAAGATTTACCCAATGGGGGAGCACAGGGAAGATGCTGGTGAAGA

164 600

K K H I E D S G E K P N T F S N K R S E A S A K K K D E S V A R A D A H S M E 1 GAAGAAACACATTGAAGATTCTGGAGAGAAGCCGAACACCTTCTCCAACAAAAGAAGCGA GGCTTCCGCTAAGAAAAAAGATGAGTCAGTGGCCAGAGCAGATGCACACTCCATGGAGC'1T

204 720

E K K T H S R E Q S S Q E S G K E T R R Q E K P Q E L T D Q D Q S Q E E S Q E G GGAGGAGAAGACACACAGTAGGGAGCAAAGCAGCCAGGAGAGTGGAGAGGAGACAAGGAG GCAGGAGAAACCCCAGGAGCTTACCGACCAGGACCAGAGCCAGGAAGAATCCCAGGAAGcV

840

E E G E E G E E G E E G E E D S A S E V T K R R P R H H H G R S G S N K S S Y F CGAGGAAGGTGAGGAAGGCGAGGAAGGCGAGGAAGGCGAGGAAGACTCAGCCTCTGAAGT AACCAAACGCAGGCCCAGACACCACCACGGGAGAAGCGGGTCCAACAAGTCCTCTTACGA

284 960

G H P L S E E R R P S P K E S K E A D V A T V R L G E K R S H H L. A H Y R A S E AGGGCATCCTCTATCTGAGGAAAGGAGACCCTCTCCCAAGGAGTCCAAGGAGGCAGATGT GGCCACAGTCCG'rTTAGGGGAAAAAAGGAGTCACCATCI'AGCTCACT'ACAGGGCTTCAGA

1080

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324

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364 1200

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404 1 320

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CTACCCTG'rTCGCGAAAGCCCAATl'AV, A I'ACV-V-VCAAAAAGGTACCCACAAAV;CAAATrGGC'A

444 1440

E Q E K N Y L N Y G E E G D Q G R W w Q Q L E Q L G P L E S R E E 'V.! R F i' D P Q AGAGCAGGAGAAAAACTACCTCAACTATGGCGAGGAAGGGGACCAAGGGAGATGGT(;GCA GCAGGAAGAGCAGCTAGGACCGGAAGAAAGCAGGGAGGAAGTAAGGT'I'TCCAGAU('AV- A('A

484 1 560

H

L

s

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TAAGAGAAACCACCCCGACTCTGAACTGGAAAGCACAGCAAACAGACATGGT'GAAGAAAC CGAGGAAGAGAGGAGC'TACGAVGVV6VVVG'AAAT1VV6ACGCCAACACAGAGGCAGGGGGCT'GGV A G A H S A 1. D T R E E K R L L D E G H AGCAGGAGCCCATTCTGCTCTTGACACCAGAGAAGAAAAAAGGCTCTTGGATGAAGGCCA

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564 1800

ATATGAGAAGAGAAGCTTTGCCAGGGCCCCTCAGCTCGACTTGAAACGGCAATATGA'TGG AGTGGCCGAGTTGGACCAGCTGCTTCACTACAGGAAGAAGGCAGACGAATTTCC('GATT'T

G

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604 1920

Y D S K E Q M G P H Q E A N D E K A R A D Q R V L T A Ft E K K E L E N L A A N D CTACGACTCGGAGGAGCAGATGGGGCCTCACCAGGAGGCAAACGATGAAAAGGCCAGGGC TGACCAGAGAGTTCTGACGGCGGAAGAGAAAAAGGAACTGGAGAACTTGGCTGCGATGGA

644 2040

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ATATGAGCCCTATCCCATCAC'I'GAAAAGAGGAAGAGGTTAGGGGCGCTGTTCAACCCVS'I'A CTTTGACCCTCTCCAGTGGAAGAATAGTGACTTTGAGAAAAGAGGCAACCCAGATGACAG TTTTCTTGAGGATGAAGGTGAAGACAGAAATGGGGTGACCTTGACTGAGAAGAATTCCTT CCCAGAGTACAACTATGACTGGTGGGAGAGAAGGCCCTTCTCAGAGGATGTGAATTGGGG

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*

TCTGGAACTCCAGAAGATAGCGGAAAAGTTCAGCCAGCGGGGCTGACAGTTGGAGAGACG AGCCT'TTCACTGAAGGACACCATTGATTCACCCACAGGCAGAAAGCAGAATTTACTGTTT

To whom

124

S

AGAAGAACCTGAATATGGGGAAGAATCGAGAAGC'rAT'CGGGGACTACAGTACCGGGG'T'AG AGGAAGCGAAGAGGACAGAGCCCCAAGGCCT'('GGAGTG'AGGAGAGTCAGGAAAGGGAGTA

*

+4

524 1680

657 2 160

ATTAAATGTTTGATGCAGTTGGAAATATCATTTATCTTTGCCAGAATGCTAAT'GCCA'I'GTV GACTAGCG'T'GACTrTGTAGCGTAT'I'T'V'TTCTGCAAAATAGATGTTTCACGTGTCTGTGACA

2280

ATGACCGTGTTACTGTTGTTGAAACACAAACAATAAAAAGATTTACCTGA- (pol y-A)

233 1

correspondence

should be addressed