Journal of Immunological Methods, 153 (1992) 261-262
261
© 1992 ElsevierSciencePublishers B.V. All rights reserved 01122-1759/92/$115.00
JIM 06459 Software notice
PROBIT: weighted probit regression analysis for estimation of biological activity M. Bailey, N.A. Williams, A.D. Wilson and C.R. Stokes Department of Veterinary.Medicine, Unirersity of Bristol, Langford House, Lungford. Bristol, BSI8 7DU, UK
PROBIT estimates biologic activity by weighted probit regression analysis and comparison with a known standard. The program is intended to speed up calculation of the concentration of cytokines in large numbers of cell superaatants. Data are input from either sequential or free-form text files created with a spreadsheet or text editor. This allows transfer of data from a beta-counter equipped with a suitable terminal without retyping. Results are displayed a n d / o r printed as relative potency (% of standard) and 50% effective dose (ED50).
Description The following example illustrates PROBIT. It assumes that the data has been loaded. The required form of the data is described in the primary information document, the file probit.txt, available as part of the package. The program first calculates the replicate means, finds the maximum and minimum values of the standard, converts to percentages, and then to probits. The user determines which values to use for regression analysis, first for the standard, and subse-
quently for samples. The resulting information is displayed in Fig. 1 for a sample data set.
Hardware/software requirements Systems: IBM PC or compatible, under PCD O S / M S - D O S . It is adequately fast on a 4.77 MHz IBM XT with 512 K. No special graphics adaptor or mathematics coprocessor is required.
Availability Correspondence to: M. Bailey, Department ol Veterinary Medicine, Universityof Bristol, Langford House, Langford, Bristol, BSI8 7DU, UK.
From the journal's ftp site, jim.mscs.mu.edu, under the directory pub/Bailey.
262
STJ~D~ []
3 4 5 6 7 8 9
eeH
-0.30103 -0.60206 -0.90309 -1.20412 -1.50815 -1.80618 -2.10721 -2.40824
105437.0 132176.5 124333.5 96167.0 94777.5 60732.5 44804.5 29149.0 7155.3
First value tot regression 3 Last ValUe £or regression 5 Provisional slope 1.54254234735 Intercept 7.71818285714 Heteregeneity: chl-sqr. 0.085, C o r r e c t e d s l o p e m 1.48552425315
Intercept ED50 -
1/
t ~ux
erobit
78.61 100o00 93.73 71.20 70.09 42.85 30.11 17.59 0.00
d.f.
5.7926 6.5301 5.5592 5.5273 4.5211 4.4785 4.0693
-
4
7.60130905872
56.3315258295
............................................................
SAMPLE Log
]
10 - 0 . 3 0 1 0 3 11 - 0 . 9 0 3 0 9 12 - ] . 5 0 5 1 5
CPM 30297.0 12469.0 4023.0
% Max
Probit
18.51 4.25 -2.51
4.1035 3.2831
F i r s t v a l u e for r e g r e s s i o n 10 L a s t v a l u e for r e g r e s s i o n 11 Heterogeneity: chl-sqr. -- 0.000, d.f. Slope 1.48582425315 d e f i n e d by s t a n d a r d Intercept m 4.57391908923 EO50 - 11 0.515697067602 P o t e n c y -- 0 . 9 1 7 2 4 3 1 6 5 0 4 1 % o~ s t a n d a r d
o
............................................................
References Finney, D.J. (1971) Probit Analysis, 3rd edn. Cambridge University Press, Cambridge, England.
Goulden, C.H., (1952) Methods of Statistical Analysis, 2nd edn. Wiley, New York
261
© 1992 ElsevierSciencePublishers B.V. All rights reserved 01122-1759/92/$115.00
JIM 06459 Software notice
PROBIT: weighted probit regression analysis for estimation of biological activity M. Bailey, N.A. Williams, A.D. Wilson and C.R. Stokes Department of Veterinary.Medicine, Unirersity of Bristol, Langford House, Lungford. Bristol, BSI8 7DU, UK
PROBIT estimates biologic activity by weighted probit regression analysis and comparison with a known standard. The program is intended to speed up calculation of the concentration of cytokines in large numbers of cell superaatants. Data are input from either sequential or free-form text files created with a spreadsheet or text editor. This allows transfer of data from a beta-counter equipped with a suitable terminal without retyping. Results are displayed a n d / o r printed as relative potency (% of standard) and 50% effective dose (ED50).
Description The following example illustrates PROBIT. It assumes that the data has been loaded. The required form of the data is described in the primary information document, the file probit.txt, available as part of the package. The program first calculates the replicate means, finds the maximum and minimum values of the standard, converts to percentages, and then to probits. The user determines which values to use for regression analysis, first for the standard, and subse-
quently for samples. The resulting information is displayed in Fig. 1 for a sample data set.
Hardware/software requirements Systems: IBM PC or compatible, under PCD O S / M S - D O S . It is adequately fast on a 4.77 MHz IBM XT with 512 K. No special graphics adaptor or mathematics coprocessor is required.
Availability Correspondence to: M. Bailey, Department ol Veterinary Medicine, Universityof Bristol, Langford House, Langford, Bristol, BSI8 7DU, UK.
From the journal's ftp site, jim.mscs.mu.edu, under the directory pub/Bailey.
262
STJ~D~ []
3 4 5 6 7 8 9
eeH
-0.30103 -0.60206 -0.90309 -1.20412 -1.50815 -1.80618 -2.10721 -2.40824
105437.0 132176.5 124333.5 96167.0 94777.5 60732.5 44804.5 29149.0 7155.3
First value tot regression 3 Last ValUe £or regression 5 Provisional slope 1.54254234735 Intercept 7.71818285714 Heteregeneity: chl-sqr. 0.085, C o r r e c t e d s l o p e m 1.48552425315
Intercept ED50 -
1/
t ~ux
erobit
78.61 100o00 93.73 71.20 70.09 42.85 30.11 17.59 0.00
d.f.
5.7926 6.5301 5.5592 5.5273 4.5211 4.4785 4.0693
-
4
7.60130905872
56.3315258295
............................................................
SAMPLE Log
]
10 - 0 . 3 0 1 0 3 11 - 0 . 9 0 3 0 9 12 - ] . 5 0 5 1 5
CPM 30297.0 12469.0 4023.0
% Max
Probit
18.51 4.25 -2.51
4.1035 3.2831
F i r s t v a l u e for r e g r e s s i o n 10 L a s t v a l u e for r e g r e s s i o n 11 Heterogeneity: chl-sqr. -- 0.000, d.f. Slope 1.48582425315 d e f i n e d by s t a n d a r d Intercept m 4.57391908923 EO50 - 11 0.515697067602 P o t e n c y -- 0 . 9 1 7 2 4 3 1 6 5 0 4 1 % o~ s t a n d a r d
o
............................................................
References Finney, D.J. (1971) Probit Analysis, 3rd edn. Cambridge University Press, Cambridge, England.
Goulden, C.H., (1952) Methods of Statistical Analysis, 2nd edn. Wiley, New York
