BIOLOGY
OF
REPRODUCTION
20, 779-792
(1979)
Progesterone Synthesis and Fine Structure Dissociated Monkey (Macaca mu latta) Luteal Cells Maintained in Culture J. GULYAS,
BELA
and
RICHARD GARY
Section Endocrinology National
Institute
of
L. STOUFFER’
D. HODGEN
on
and
Endocrinology,
Reproduction
Child
National
of
Research
Health
and
Institutes
Bethesda,
Development,
Health,
of
Maryland
Branch,
Human
20205
ABSTRACT
The
process
of functional
regression
of the primate
corpus
well understood, yet it remains an important determinant ceptive actions. Accordingly, the feasibility of maintaining long term studies was explored. When midluteal phase
luteum in the menstrual cycle is not in fertility management, including contrarhesus monkey luteal cells in culture for corpus luteum (CL) was dispersed with
in Ham’s FlO (FlO) medium, 60% of the initial 5 X io cells plated attached. Unattached cells were removed in media changes on Day 2 and 4. Trypan blue exclusion showed that 60-80% of unattached cells were dead or dying. At 10 days of culture, the number of cells per dish in FlO was comparable to the number of cells when D-valine was substituted for L-valine (D-val-F10) and when 100 ng hCG/ml was added to FlO. The number of cells was slightly higher when cultured in Hank’s M199 (M199) medium. Progesterone (P) production in FlO medium declined markedly between Day 2 and Day 6, but remained detectable until Day 10. Although hCG increased P production (P
OF
REPRODUCTION
20, 779-792
(1979)
Progesterone Synthesis and Fine Structure Dissociated Monkey (Macaca mu latta) Luteal Cells Maintained in Culture J. GULYAS,
BELA
and
RICHARD GARY
Section Endocrinology National
Institute
of
L. STOUFFER’
D. HODGEN
on
and
Endocrinology,
Reproduction
Child
National
of
Research
Health
and
Institutes
Bethesda,
Development,
Health,
of
Maryland
Branch,
Human
20205
ABSTRACT
The
process
of functional
regression
of the primate
corpus
well understood, yet it remains an important determinant ceptive actions. Accordingly, the feasibility of maintaining long term studies was explored. When midluteal phase
luteum in the menstrual cycle is not in fertility management, including contrarhesus monkey luteal cells in culture for corpus luteum (CL) was dispersed with
in Ham’s FlO (FlO) medium, 60% of the initial 5 X io cells plated attached. Unattached cells were removed in media changes on Day 2 and 4. Trypan blue exclusion showed that 60-80% of unattached cells were dead or dying. At 10 days of culture, the number of cells per dish in FlO was comparable to the number of cells when D-valine was substituted for L-valine (D-val-F10) and when 100 ng hCG/ml was added to FlO. The number of cells was slightly higher when cultured in Hank’s M199 (M199) medium. Progesterone (P) production in FlO medium declined markedly between Day 2 and Day 6, but remained detectable until Day 10. Although hCG increased P production (P
