IN NEURAL
PROSTAGLANDINS N.
PAPANICOLAOU,
Th.
J.
CREST-TU!!OURS
F1OUNTOKALAKIS,
BARIETY
and
P.
Ph.
Groupe
de
Recherches
sur
la
Centre
de
Recherches
sur
1'Hypertension
I.N.S.E.R.M.
U
28
-
HOPITAL
TCHERBAKOFF,
MILLIE7
Pathologie
Renale
et
vasculaire
Arterielle,
BROUSSAIS,
P A
Ii I S
75014
and C.
BOHUON,
Institut
6.
TCHERRIA,
0.
ROUSSY,
Gustave
SCHWEISGOUTH
94800
VILLEJUIF
ABSTRACT Prostaglandin were
estimated
in
and tumour
phaeochromocytomas
and
Prostaglandins associated
were and
Prostaglandin were
in
The
hypothesis
not
reach
gical present
as
has
other to
well
been
arterial
be
as
as
present
incidence
of
in
chronic
catecholamine,
made
than that
chronic
phaeochromocytomas synthesis
concentrations from
to
hypertension
while give
tumours
both
diarrhoea.
the
other
tumours.
prostaglandins rise
to
do
pharmacolo-
prostaglandins
contribute
release,
tumours.
concentrations in
diarrhoea., may
and/or
four
neural-crest
with
circulation
such
catecholamine higher
found
taken
phaeochromocytomas
effects, in
five
non-associated F,
higher
catecholamine tissues
to
resultinq
associated
with
a
F
higher in
a
these
tumours.
PROSTAGLANDINS SEPTEMBER
1975
VOL. 10 NO. 3
405
PROSTAGLANDINS
INTRODUCTION Although gic
origin
phaeochromocytomas
neuroblastomas,
neuroblastomas,
hypertension
phaeochromocytoma crest
but
tumours,
ciation only
while
with
with
Prostaglandins
mas,
but
their
of
with
chronic
crest
features
a g
for
of
tissue
dissolved When of
the the
in
detail to
PGA2,
in
2 ml
of
Krebs
of
the
response
eluate
than
that
half
was
to
The
assayed. determined metry
406
(4
100
of
diluted
tissular
by -
mg
column
reported
the
of
obtained
at
standard with
10
concentration chromatography
to g of
from
the
quantibeen
TLC
areas
spots
were
bioassay.
first
tissue)
solution
used
and
before
of
Usally
have
prostaglandins, Krebs
ca-
surqery
tumours.
standard
solution-just organs
and
procedures
Eluates
PGF?,
and
neural-
prostaglandin
neural
to
the
secretion
prostaglandins
assay
occuren-
associated
tumour.The
and
remains
investigate
tissues
(3).
taken
syndromes
tumours
of
elsewhere PGE2
assoand
tissues
identification
(corresponding
further
we
further
each
of
in
phaeochromocyto-
tumour
functioning
estimation
corresponding
tumour
sympathetic
from
neural-
occur
prostaglandin
chromatographic
bioassay
described
To
analysis
taken
to
and
study
in
these
was
extraction,
tative
the an
with
other
ganglioneuroblastoma
(2).
did
patients
phaeochromocytoma.
in
various
concentrations
number
of
a
embryolo-
ganglio-
ganglioneuroblastoma and
between of
we
techolamine
20
in
in
known
assayed
the
diarrhoea
origin,
from
in
relationship
clinical
is
a previous
In
common
with
neuroblastomas
prostaglandins
possible
common
and
been
role
(1).
a
and
patients
diarrhoea
have
ganglioneuromas,
ce
in
neuroblastoma
from
uncertain
is
rare
ganglioneuroma
rarely
have
ganglioneuromas
half was
the and
more second
then
catecholamines
and
rewas
spectrophotofluoro-
6).
SEPTEMBER
1975 VOL. 10 NO. 3
PROSTAGLANDINS
RESULTS The amounts could
are
while
no
in four
associated
with
as well
noradrenaline
as
than
chronic yere
in the
0)
*r w Ia P
-
L
%
s E
aJ
z
B ia)
. .
z
Cl
_c L
(mmHg)
2 .r
:
cases.
and/or
n
Detectable
found
in most
both
E and
associated
Prostaglandin
adrenaline higher
neural-crest
tumour
of prostaglandins
Prostaglandins
in tumours
and
(b) ;1 a
F were
diarrhoea.
other
table.
amounts
considerably
Prostaglandins
:: t
E and
to be present
concentrations,
in the
identifiable
be detected found
tomas
shown
of prostaglandins
tissues, were
results
and
A F
and
nor
F levels, dopamine
in phaeachromocy-
tumours.
Catecholamines
in Tumour
tissues
Prostaglandins
Catecholamines
(rig/g tissue)
(rg/g
PGA
PGE
PGF
tissues)
NA
A
DA
100/60
Yes
4.0
26.0
6.0
1.3
1.9
0.9
GN
95/50
Yes
N D 15.0
10.0
0.1
2.0
1.4
1
NB
90/50
No
N D
8.0
20.0
0.2
N D
14.8
A.G.
4
NB
100/50
No
6.0
12.0
10.0
0.1
ND
0.3
A.P.
1
NB
95/60
No
5.0
7.0
4.0
0.4
1.9
4.3
B.M.
60
PC
27G/150
No
N 3 52.0
107.0
1.0
211.0
16.0
G.G.
37
PC
230/130
No 20.0
10.0
37.0
1360.0
1.0
11.2
N.G.
33
PC
270/15D
No 30.0
12.0
50.0
1725.0
2403.0
50.5
L.J.
40
PC
245/120
No
N D
33.0
331?.0
6996.0
124.0
G.N.
6
GNB
R.V.
2
C.P.
GNB NB
: Ganglioneuroblastoma : Neuroblastoma
SEPTEMBER
1975
; PC
N D
; GN
: Ganglioneuroma
: Phaeochromocytoma
VOL. 10 NO. 3
;
;
407
PROSTAGLANDINS
1iA : bioradrenaline N
D
: Non
; A : Adrenaline
: Dopamine
; DA
;
detectable.
0)
: Ilean
(b)
: Up
values
from
several
measurements
in
a
supine
position. to
10
contain
liquid
stools
blood,
indigested
pus
per
day.
cells,
Stools
did
pathogenic
not
bacteria
or
food. DISCUSSION
High found that
in
not
or
with
other
such
substance
do
must
be
not
tissues
represent generation.
tumours
both
suggests
that the
gical
effect.
knowledge ly
animal
unchanged
humoral
ved
in
and
their 8).
the
tissue
lo),
to is
(11, factor
12). other
occasional
in
In
passage there
its
diarrhoea
to
A
with
almost
the
pulmonary
that
pass
through
role
of
in
the
this
lung
qroup has
tumours,
been
therefore,
might
chronic
not
completethe
self-existence
of
do
pharmacolo-
evidence
protaglandins
development
rate,
in
tumours
throuqh is
a
prosta-
with
are
of
in
accordance
neural-crest than
levels
in
rise
F
do
secretion
by
give
physiological since
its
is
stud:/
prostaglandins
and
prostaglandins the
neoplams
tissue
contained
single
such
present
capacity
of
E
the
with
non-associated
Although
controversial,
questioned
the
suggestion
species (9,
remains
a
(7,
to
been
evidence
concentration
presence
circulation
on
bed
the
represent
prostaglandins
inactivated
most
The
This
related
Although
prostaglandins
that
vascular
rather
arterial
of
have
the
patients
prostaglandin
associated
reach
results
necessarely that
glandin
F
However,
be
in
hypothesis.
noted
E and
amine-peptide-secreting The
a
may
observed
inconclusive. favour
(1).
secretion
occasionaly
tumours
prostaglandins
tumours
prostaglandin
still
of
neural-crest
diarrhoea
it
levels
be
invol-
diarrhoeal
syndrome.
408
SEPTEMBER
1975
VOL. 10 NO. 3
PROSTAGLANDINS
no
Evidently, the
possible
prostaglandin
mas,
can
be
drawn
could
associated tic
volume
of
lation
these
rather in
of
the
sodium
strengthened
exerted sites
normotensive
by
a
ry
output
our
to
tion.
Thus,
14,
modify
glandins
(13)
the
storage
nes
has
is
inhibit
facilitate
been
A
are
(19).
that
prostaglandins
nes of
into
to
a
release the
F
the
higher of
1975
into
A
and
urina-
are circulaprostaF
difference of to
the
fact only
with
could,
hyperten-
therefore,
phaeochromocytomas
catecholamine
resulting these
in
catecholani-
tumours
be may
synthesis
and/or
active
catecholami-
pharmacologically
VOL. 10 NO. 3
accompanied
the
associated
in
and
prostaglandins
oroducing
with
rat
that
release
hypothesis
associated
is
explanation
present
circulation,
hypertension
SEPTEMBER
an
release
the
evidence
commonly
speculative
sion
higher
as
is
prostaglandins
release. in
catecholamines
phaeochromocytomas
contribute
and
regu-
haemodynaaics
whereas
noradrenaline
proposed
all
17),
by
suggestion
in
release
blood
peripheral
prostaglandin
However,
(16,
and
and
This
experimental
mechanism
from
renal
pressure
homoeasta-
of
subjects
catecholanine
there
E
of
the
expansion
hypertensive
15).
neural
pressure
synthesis
that
the
present
blood
decrease
their
space
increase
(13,
in
fact,
balance.
findings
and
parallel
known
a
of
a
the
functioning
blood
by
water
of
changes In
of
phaeochromoc,vto-
data
in
to
on
extracellular
in
the
tumours.
and
by
following
in
contained
prostaglandins
seems
resistance
from
unrelated
regarding
implication
concentrations
solely
be
with
role
that
F
Prostaglandins
tumours
conclusion,
physio-pathological
high
study.
clear-cut
in
a
hiqher
turnours.
to
incidence
PROSTAGLANDINS
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