Advanced Pharmaceutical Bulletin
Adv Pharm Bull, 2014, 4(Suppl 2), 493-499 doi: 10.5681/apb.2014.073 http://apb.tbzmed.ac.ir
Research Article
Protective Effects of Crocus Sativus L. Extract and Crocin against Chronic-Stress Induced Oxidative Damage of Brain, Liver and Kidneys in Rats Ahmad Reza Bandegi1,2§, Ali Rashidy-Pour1§, Abbas Ali Vafaei1*, Behshid Ghadrdoost1 1
Laboratory of Learning and Memory, Research Center and Department of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran. 2 Laboratory of Endocrine Research, Department of Biochemistry, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Article History: Received: 20 February 2014 Revised: 26 November 2014 Accepted: 29 November 2014 ePublished: 31 December 2014
Keywords: Chronic stress Brain Liver Kidney Saffron Extract and Crocin Oxidative stress
Abstract Purpose: Chronic stress has been reported to induce oxidative damage of the brain. A few studies have shown that Crocus Sativus L., commonly known as saffron and its active constituent crocin may have a protective effect against oxidative stress. The present work was designed to study the protective effects of saffron extract and crocin on chronic – stress induced oxidative stress damage of the brain, liver and kidneys. Methods: Rats were injected with a daily dose of saffron extract (30 mg/kg, IP) or crocin (30 mg/kg, IP) during a period of 21 days following chronic restraint stress (6 h/day). In order to determine the changes of the oxidative stress parameters following chronic stress, the levels of the lipid peroxidation product, malondialdehyde (MDA), the total antioxidant reactivity (TAR), as well as antioxidant enzyme activities glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD) were measured in the brain, liver and kidneys tissues after the end of chronic stress. Results: In the stressed animals that receiving of saline, levels of MDA, and the activities of GPx, GR, and SOD were significantly higher (P
Adv Pharm Bull, 2014, 4(Suppl 2), 493-499 doi: 10.5681/apb.2014.073 http://apb.tbzmed.ac.ir
Research Article
Protective Effects of Crocus Sativus L. Extract and Crocin against Chronic-Stress Induced Oxidative Damage of Brain, Liver and Kidneys in Rats Ahmad Reza Bandegi1,2§, Ali Rashidy-Pour1§, Abbas Ali Vafaei1*, Behshid Ghadrdoost1 1
Laboratory of Learning and Memory, Research Center and Department of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran. 2 Laboratory of Endocrine Research, Department of Biochemistry, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Article History: Received: 20 February 2014 Revised: 26 November 2014 Accepted: 29 November 2014 ePublished: 31 December 2014
Keywords: Chronic stress Brain Liver Kidney Saffron Extract and Crocin Oxidative stress
Abstract Purpose: Chronic stress has been reported to induce oxidative damage of the brain. A few studies have shown that Crocus Sativus L., commonly known as saffron and its active constituent crocin may have a protective effect against oxidative stress. The present work was designed to study the protective effects of saffron extract and crocin on chronic – stress induced oxidative stress damage of the brain, liver and kidneys. Methods: Rats were injected with a daily dose of saffron extract (30 mg/kg, IP) or crocin (30 mg/kg, IP) during a period of 21 days following chronic restraint stress (6 h/day). In order to determine the changes of the oxidative stress parameters following chronic stress, the levels of the lipid peroxidation product, malondialdehyde (MDA), the total antioxidant reactivity (TAR), as well as antioxidant enzyme activities glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD) were measured in the brain, liver and kidneys tissues after the end of chronic stress. Results: In the stressed animals that receiving of saline, levels of MDA, and the activities of GPx, GR, and SOD were significantly higher (P
