Cellular Physiology and Biochemistry
Cell Physiol Biochem 2014;33:1643-1653 DOI: 10.1159/000362947 Published online: May 16, 2014
© 2014 S. Karger AG, Basel www.karger.com/cpb
Rui et al.: Radix Extract Protects Retinal Laser Injury Accepted: April Pseudostellariae 28, 2014 1421-9778/14/0336-1643$39.50/0 This is an Open Access article licensed under the terms of the Creative Commons AttributionNonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only.
Original Paper
Protective Effects of Radix Pseudostellariae Extract Against Retinal Laser Injury Guo Ruia,e Wei Weia,e Wang Yuliangb,e Li Kaib Cui Xiaobingc Zhu Changled Shen Longshud Department of Ophthalmology, Nanjing University of Chinese Medicine, bDepartment of Ophthalmology, the First Affiliated Hospital of Nanjing University of Chinese Medicine, cCollege of Pharmacy, Nanjing University of Chinese Medicine, dDepartment of Pathology, the First Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China; eThese authors contributed equally to this work (co-first author) a
Key Words Radix Pseudostellariae • Frequency-doubled 532 nm Nd:YAG laser • Retina Abstract Background: This study aimed to analyze the protective effects of a saponin extract from Radix Pseudostellariae (RP) on retinal laser injury based on a retinal photocoagulation model. Methods: Fifty-eight rabbits were randomly divided into three groups: Group A (saponin extract orally), Group B (physiological saline), and Group C (control). The animals were sacrificed 1 day, 7 days, 14 days, and 30 days after photocoagulation and lesions were evaluated with fundus photography, light microscopy, and electron microscopy. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured, and expression levels of c-fos and Bax genes were also determined. Results: The lesion sizes in Group A were smaller than in Group B. The levels of SOD in Group B were significantly lower than in groups A and C (P
Cell Physiol Biochem 2014;33:1643-1653 DOI: 10.1159/000362947 Published online: May 16, 2014
© 2014 S. Karger AG, Basel www.karger.com/cpb
Rui et al.: Radix Extract Protects Retinal Laser Injury Accepted: April Pseudostellariae 28, 2014 1421-9778/14/0336-1643$39.50/0 This is an Open Access article licensed under the terms of the Creative Commons AttributionNonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only.
Original Paper
Protective Effects of Radix Pseudostellariae Extract Against Retinal Laser Injury Guo Ruia,e Wei Weia,e Wang Yuliangb,e Li Kaib Cui Xiaobingc Zhu Changled Shen Longshud Department of Ophthalmology, Nanjing University of Chinese Medicine, bDepartment of Ophthalmology, the First Affiliated Hospital of Nanjing University of Chinese Medicine, cCollege of Pharmacy, Nanjing University of Chinese Medicine, dDepartment of Pathology, the First Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China; eThese authors contributed equally to this work (co-first author) a
Key Words Radix Pseudostellariae • Frequency-doubled 532 nm Nd:YAG laser • Retina Abstract Background: This study aimed to analyze the protective effects of a saponin extract from Radix Pseudostellariae (RP) on retinal laser injury based on a retinal photocoagulation model. Methods: Fifty-eight rabbits were randomly divided into three groups: Group A (saponin extract orally), Group B (physiological saline), and Group C (control). The animals were sacrificed 1 day, 7 days, 14 days, and 30 days after photocoagulation and lesions were evaluated with fundus photography, light microscopy, and electron microscopy. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured, and expression levels of c-fos and Bax genes were also determined. Results: The lesion sizes in Group A were smaller than in Group B. The levels of SOD in Group B were significantly lower than in groups A and C (P
