Accepted Manuscript Title: Radiation therapy alters microhardness and microstructure of enamel and dentin of permanent human teeth Author: Ligia Maria Napolitano Gonc¸alves Regina Guenka Palma-Dibb Francisco Wanderley Garcia Paula-Silva Harley Francisco de Oliveira Paulo Nelson-Filho L´ea Assed Bezerra da Silva Alexandra Mussolino de Queiroz PII: DOI: Reference:
S0300-5712(14)00155-9 http://dx.doi.org/doi:10.1016/j.jdent.2014.05.011 JJOD 2300
To appear in:
Journal of Dentistry
Received date: Revised date: Accepted date:
26-11-2013 21-5-2014 23-5-2014
Please cite this article as: Gonc¸alves LMN, Palma-Dibb RG, Paula-Silva FWG, Oliveira HF, Nelson-Filho P, Silva LAB, Queiroz AM, Radiation therapy alters microhardness and microstructure of enamel and dentin of permanent human teeth, Journal of Dentistry (2014), http://dx.doi.org/10.1016/j.jdent.2014.05.011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Radiation therapy alters microhardness and microstructure of enamel and dentin of permanent human teeth
cr
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Radiation therapy alters enamel and dentin of permanent teeth
Ligia Maria Napolitano Gonçalves,1 Regina Guenka Palma-Dibb,2 Francisco Wanderley
an
Bezerra da Silva,2 Alexandra Mussolino de Queiroz2*
us
Garcia Paula-Silva,2 Harley Francisco de Oliveira,3 Paulo Nelson-Filho,2 Léa Assed
Pediatric Dentistry MSc Student and 2Pediatric Dentistry Graduate Program Professor
M
1
at School of Dentistry of Ribeirao Preto at University of Sao Paulo. 3
Ac ce p
te
d
Professor at School of Medicine of Ribeirao Preto at University of Sao Paulo.
*Corresponding author:
Faculdade de Odontologia de Ribeirão Preto da Universidade de São Paulo. Avenida do Café, s/n. 14040-904. Ribeirão Preto. SP. Brazil. Phone: 55 (16) 3602-4116. Fax: 55 (16) 3602-4102. e-mail: [email protected]
Key Words: radiotherapy; permanent dentition; microhardness; scanning electron microscopy.
Page 1 of 23
Abstract Purpose: To evaluate, in vitro, the effects of ionizing radiation on the mechanical and
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micro-morphological properties of enamel and dentin of permanent teeth. Material and Methods: Enamel and dentin microhardness (n=12 hemi-sections) was evaluated at
cr
three depths (superficial, middle and deep) prior to (control) and after every 10 Gy
radiation dose up to a cumulative dose of 60 Gy by means of longitudinal
us
microhardness. Data were analyzed using two-way analysis of variance and Tukey’s test at a significance level of 5%. Enamel and dentin morphology was assessed by
an
scanning electron microscopy (SEM) for semi-quantitative analysis (n=8 hemisections). Data were analyzed using Kruskal Wallis and Dunn’s or Fisher exact tests at
M
a significance level of 5%. Results: The application of ionizing radiation did not change the overall enamel microhardness, although an increase in superficial enamel
d
microhardness was observed. The micro-morphological analysis of enamel revealed that
te
irradiation did not influence rod structure but interprismatic structure became more
Ac ce p
evident. Dentin microhardness decreased after 10, 20, 30, 50 and 60 Gy cumulative doses (p
S0300-5712(14)00155-9 http://dx.doi.org/doi:10.1016/j.jdent.2014.05.011 JJOD 2300
To appear in:
Journal of Dentistry
Received date: Revised date: Accepted date:
26-11-2013 21-5-2014 23-5-2014
Please cite this article as: Gonc¸alves LMN, Palma-Dibb RG, Paula-Silva FWG, Oliveira HF, Nelson-Filho P, Silva LAB, Queiroz AM, Radiation therapy alters microhardness and microstructure of enamel and dentin of permanent human teeth, Journal of Dentistry (2014), http://dx.doi.org/10.1016/j.jdent.2014.05.011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Radiation therapy alters microhardness and microstructure of enamel and dentin of permanent human teeth
cr
ip t
Radiation therapy alters enamel and dentin of permanent teeth
Ligia Maria Napolitano Gonçalves,1 Regina Guenka Palma-Dibb,2 Francisco Wanderley
an
Bezerra da Silva,2 Alexandra Mussolino de Queiroz2*
us
Garcia Paula-Silva,2 Harley Francisco de Oliveira,3 Paulo Nelson-Filho,2 Léa Assed
Pediatric Dentistry MSc Student and 2Pediatric Dentistry Graduate Program Professor
M
1
at School of Dentistry of Ribeirao Preto at University of Sao Paulo. 3
Ac ce p
te
d
Professor at School of Medicine of Ribeirao Preto at University of Sao Paulo.
*Corresponding author:
Faculdade de Odontologia de Ribeirão Preto da Universidade de São Paulo. Avenida do Café, s/n. 14040-904. Ribeirão Preto. SP. Brazil. Phone: 55 (16) 3602-4116. Fax: 55 (16) 3602-4102. e-mail: [email protected]
Key Words: radiotherapy; permanent dentition; microhardness; scanning electron microscopy.
Page 1 of 23
Abstract Purpose: To evaluate, in vitro, the effects of ionizing radiation on the mechanical and
ip t
micro-morphological properties of enamel and dentin of permanent teeth. Material and Methods: Enamel and dentin microhardness (n=12 hemi-sections) was evaluated at
cr
three depths (superficial, middle and deep) prior to (control) and after every 10 Gy
radiation dose up to a cumulative dose of 60 Gy by means of longitudinal
us
microhardness. Data were analyzed using two-way analysis of variance and Tukey’s test at a significance level of 5%. Enamel and dentin morphology was assessed by
an
scanning electron microscopy (SEM) for semi-quantitative analysis (n=8 hemisections). Data were analyzed using Kruskal Wallis and Dunn’s or Fisher exact tests at
M
a significance level of 5%. Results: The application of ionizing radiation did not change the overall enamel microhardness, although an increase in superficial enamel
d
microhardness was observed. The micro-morphological analysis of enamel revealed that
te
irradiation did not influence rod structure but interprismatic structure became more
Ac ce p
evident. Dentin microhardness decreased after 10, 20, 30, 50 and 60 Gy cumulative doses (p
