Radioimmunoassay for Luteinizing Hormone-Releasing Hormone (LHRH): Its Application to the Measurement of LHRH in Ovine and Human Plasma1*2'3 HELEN A. JONAS, HENRY G. BURGER, IAN A. CUMMING, JOHN K. FINDLAY, AND DAVID M. DE KRETSER, WITH THE TECHNICAL ASSISTANCE OF JULIE ROBINSON
Medical Research Centre, Prince Henry's Hospital, Melbourne, 3004, Department of Agriculture, Victoria and the Reproduction Research Section, University of Melbourne, S. S. Cameron Laboratory, Werribee 3030, Victoria, Australia ABSTRACT. A radioimmunoassay for LHRH, modified from the assay of Nett et al. (J Clin Endocrinol Metab 36: 880, 1973) and characterized in more detail, was applied to the measurement of endogenous plasma LHRH in the peripheral circulation of ewes and women around the time of gonadotropin release. Sensitivity was 1.2 pg/tube. Withinand between-assay coefficients of variation were 10% and 25%, respectively, over' the range 10-100 pg/tube. Minimal cross reaction (
Medical Research Centre, Prince Henry's Hospital, Melbourne, 3004, Department of Agriculture, Victoria and the Reproduction Research Section, University of Melbourne, S. S. Cameron Laboratory, Werribee 3030, Victoria, Australia ABSTRACT. A radioimmunoassay for LHRH, modified from the assay of Nett et al. (J Clin Endocrinol Metab 36: 880, 1973) and characterized in more detail, was applied to the measurement of endogenous plasma LHRH in the peripheral circulation of ewes and women around the time of gonadotropin release. Sensitivity was 1.2 pg/tube. Withinand between-assay coefficients of variation were 10% and 25%, respectively, over' the range 10-100 pg/tube. Minimal cross reaction (
