Data in Brief 14 (2017) 123–131
Contents lists available at ScienceDirect
Data in Brief journal homepage: www.elsevier.com/locate/dib
Data Article
Raw data of the effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity Alarcón-Herrera Norberto a, Flores-Maya Saúl a, Bellido Belén b, García-Bores Ana M.c, Mendoza Ernesto b, Ávila-Acevedo Guillermo c, Hernández-Echeagaray Elizabeth b,n a Laboratorio de Recursos Naturales, UBIPRO, FES-Iztacala, Universidad, Nacional Autónoma de México, México b Laboratorio de Neurofisiología del desarrollo y la neurodegeneración UBIMED, FES-Iztacala, Universidad, Nacional Autónoma de México, México c Laboratorio de Fitoquímica, UBIPRO, FES-Iztacala, Universidad, Nacional Autónoma de México, México
a r t i c l e i n f o
abstract
Article history: Received 9 May 2017 Received in revised form 12 June 2017 Accepted 5 July 2017 Available online 12 July 2017
The raw data showed in this article comes from the published research article entitled “Protective effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity” Food Chem Toxicol. 2017 May 3. pii: S0278-6915(17)30226-0. http://dx. doi.org/DOI:10.1016/j.fct.2017.04.048. [1]. Data illustrates antitoxic and antigenotoxic effects of Chlorogenic acid (CGA) on toxicity and genotoxicity produced by the in vivo treatment with mitochondria toxin 3-Nitropropionic acid (3-NP) in mice. Toxicity and genotoxicity was evaluated in erythrocytes of peripheral blood through the micronuclei assay. Data was share at the Elsevier repository under the reference number FCT9033. & 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: 3-NP CGA Toxicity Genotoxicity Data
n
DOI of original article: http://dx.doi.org/10.1016/j.fct.2017.04.048 Corresponding author. E-mail address: [email protected] (H.-E. Elizabeth).
http://dx.doi.org/10.1016/j.dib.2017.07.004 2352-3409/& 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
124
A.-H. Norberto et al. / Data in Brief 14 (2017) 123–131
Specifications Table Subject area More specific subject area Type of data How data was acquired
Data format Experimental factors
Experimental features Data source location Data accessibility
Biology Toxicology Tables Data from erythrocytes from peripheral blood, stained with H&E were classified in Normochromatic, Polychromatic or Polychromatic with micronuclei cells. 1000 cells per each condition were counted with the aid of by the aid of an optic microscope (Leica, Microsystems AG). Raw data In order to determine toxicity and genotoxicity of 3-NP, as well as protective effects of CGA, 6 experimental groups were evaluated; Negative control, Control (PB), 3-NP, CGA, 3-NP þ CA, P/CA, 3-NP þ CA and P/CA, 3-NP Each treatment lasted for 5 days except those where 5 days of pretreatment was present. To evaluate toxic and genotoxic effect of 3-NP and the antitoxic and antigenotoxic effects of CGA in erythrocytes. México City, México Data are available in this article and were place also in a public repository provided by Elsevier submission system, under reference number FCT9033
Value of the data
Data displays Normochromatic, Polychromatic or Polychromatic cells with micronuclei in the different experimental conditions and can be used by other research groups.
Toxicity and Genotoxicity were measured in peripheral blood by using the micronuclei assay. Τhese data are important because few studies have carried out to evaluate toxicity and genotoxicity of 3-NP which can be found in plants like sugar cane that are eaten by cattle and humans.
Data about CGA protective effects are important because CGA is found in a variety of food products which can help to protect the health of living organisms.
1. Data Raw data presented in this paper gives information about protective role of CGA on toxic and genotoxic effects of 3-NP in erythrocytes from peripheral blood. Data are shown in Tables 1–7 which illustrate the effect in each evaluated time.
2. Experimental design, materials and methods To evaluate toxic effect of 3-NP and the antitoxic effect of Chlorogenic acid, mice were randomly assigned to one of the following experimental groups: negative control, ifosfamide (positive control), buffer, 3-nitropropionic acid (3-NP), Chlorogenic acid (CGA), 3-NP þ Chlorogenic acid without pretreatment (3-NP þ CA, W/P), 3-NP þ Chlorogenic acid together with pretreatment of CA per 5 days (P/CA, 3-NP þ CA), pretreatment with CA for 5 days and later treatment of 3-NP alone, (P/CA, 3-NP). All groups were treated for 5 days with i.p. doses of 3-NP (15 mg/kg), CGA (100 mg/kg).
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Table 1 Data obtained at 24 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 24 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
808 803 784 798 813
192 197 216 202 187
5 5 4 2 2
19.2 19.7 21.6 20.2 18.5
0.5 0.5 0.4 0.2 0.2
Treatment Animal 1 2 3 4 5
Ifosfamide CN 860 875 801 855 831
PC 149 125 199 145 169
PMNC 21 22 25 20 13
%TOX 14.9 12.5 19.9 14.5 16.9
%GEN 2.1 2.2 2.5 2 1.3
Treatment Animal 1 2 3 4 5
Buffer CN 811 787 771 814 805
PC 189 213 229 186 195
PMNC 3 5 2 4 3
%TOX 18.9 21.3 22.9 18.6 19.5
%GEN 0.3 0.5 0.2 0.4 0.3
Treatment Animal 1 2 3 4 5
3-NP CN 878 889 895 917 933
PC 122 111 105 83 97
PMNC 20 19 15 15 13
%TOX 12.2 11.1 10.5 8.3 9.7
%GEN 2 1.9 1.5 1.5 1.3
Treatment Animal 1 2 3 4 5
CGA CN 835 828 817 810 812
PC 165 172 183 190 188
PMNC 3 5 4 3 2
%TOX 16.5 17.2 18.3 19 18.8
%GEN 0.3 0.5 0.4 0.3 0.2
Treatment Animal 1 2 3 4 5
3NP þ CGA CN 843 840 835 810 812
PC 157 160 165 190 188
PMNC 10 10 13 11 12
%TOX 15.7 16.0 16.5 19.0 18.8
%GEN 1 1 1.3 1.1 1.2
Treatment
P/CGA, 3NP þ CGA CN 797 813 801 796 807 830 839 848 885 855
PMNC 6 8 3 3 5 11 9 10 8 8
%TOX 20.3 18.7 19.9 20.4 19.3 17 16.1 15.2 11.5 14.5
%GEN 0.6 0.8 0.3 0.3 0.5 1.1 0.9 1 0.8 0.8
Animal 1 2 3 4 5 1 2 3 4 5
PC 203 187 199 204 193 170 161 152 115 145
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A.-H. Norberto et al. / Data in Brief 14 (2017) 123–131
Table 2 Data obtained at 48 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 48 h Treatment Animal
Negative control CN
PC
PMC
%TOX
%GEN
1 2 3 4 5
789 783 810 801 810
211 217 190 194 190
5 3 6 5 1
21.1 21.7 19 19.4 19
0.5 0.3 0.6 0.5 0.1
Treatment Animal 1 2 3 4 5
Ifosfamide CN 857 862 877 859 865
PC 143 148 123 141 135
PMNC 15 17 15 18 18
%TOX 14.3 14.8 12.3 14.2 13.5
%GEN 1.5 1.7 1.5 1.8 1.8
Treatment Animal 1 2 3 4 5
Buffer CN 817 807 760 820 790
PC 183 193 240 180 210
PMNC 5 6 5 5 5
%TOX 18.3 19.3 24 18 21
%GEN 0.5 0.6 0.5 0.5 0.5
Treatment Animal 1 2 3 4 5
3-NP CN 870 891 816 850 877
PC 130 109 184 150 123
PMNC 11 10 13 10 10
%TOX 13 10.9 18.4 15 12.3
%GEN 1.1 1 1.3 1 1
Treatment Animal 1 2 3 4 5 Treatment Animal 1 2 3 4 5
CGA CN 793 806 807 800 810 3-NP þ CGA CN 847 855 833 832 827
PC 207 194 193 200 190
PMNC 4 7 5 6 5
%TOX 20.7 19.4 19.3 20 19
%GEN 0.4 0.7 0.5 0.6 0.5
PC 153 165 167 168 183
PMNC 10 11 10 11 12
%TOX 15.3 16.5 16.7 16.8 18.3
%GEN 1 1.1 1 1.1 1.2
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 797 830 794 788 799
PC 203 170 206 212 201
PMNC 2 4 2 6 5
%TOX 20.3 17 20.6 21.2 20.1
%GEN 0.2 0.4 0.2 0.6 0.5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 857 818 870 876 820
PC 163 182 130 128 180
PMNC 7 8 10 2 6
%TOX 16.3 18.2 13 12.8 18
%GEN 0.7 0.8 1 0.2 0.6
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Table 3 Data obtained at 72 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 72 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
794 799 799 791 795
205 201 201 209 205
3 3 3 2 3
20.5 20.1 20.1 20.9 20.5
0.3 0.3 0.3 0.2 0.3
Treatment Animal 1 2 3 4 5
Ifosfamide CN 832 838 751 885 783
PC 168 162 249 175 217
PMNC 25 26 25 24 17
%TOX 16.8 16.2 24.9 17.5 21.7
%GEN 2.5 2.6 2.5 2.5 1.7
Treatment Animal 1 2 3 4 5
Buffer CN 803 801 756 826 800
PC 197 199 244 174 200
PMNC 5 4 5 4 5
%TOX 19.7 19.9 24.4 17.4 20
%GEN 0.5 0.4 0.5 0.4 0.5
Treatment Animal 1 2 3 4 5
3-NP CN 879 862 860 838 855
PC 121 138 140 162 145
PMNC 20 17 17 19 17
%TOX 12.1 13.8 14 16.2 14.5
%GEN 2 1.7 1.7 1.9 1.7
Treatment Animal 1 2 3 4 5
CGA CN 799 827 785 808 800
PMNC 4 5 4 8 6
%TOX 20.1 17.3 21.5 19.2 20
%GEN 0.4 0.5 0.4 0.8 0.6
Treatment Animal 1 2 3 4 5
3-NP þ CGA CN 820 821 816 810 815
PMNC 9 9 12 11 10
%TOX 18 17.9 18.4 19 8.5
%GEN 0.9 0.9 1.2 1.1 1
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 790 795 796 789 797
PC 210 205 204 211 203
PMNC 5 3 2 3 4
%TOX 21 20.5 20.4 21.1 20.3
%GEN 0.5 0.3 0.2 0.3 0.4
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 846 832 868 857 849
PC 154 168 132 143 151
PMNC 7 7 10 5 9
%TOX 15.4 16.8 13.2 14.3 15.1
%GEN 0.7 0.7 1 0.5 0.9
PC 201 173 215 192 200 PC 180 179 184 190 185
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Table 4 Data obtained at 96 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 96 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
787 813 817 793 799
213 187 183 207 201
5 3 1 6 5
21.3 18.7 18.3 20.7 20.1
0.5 0.3 0.1 0.6 0.5
Treatment Animal 1 2 3 4 5
Buffer CN 804 792 788 810 803
PC 196 208 212 190 197
PMNC 6 5 3 4 5
%TOX 19.6 20.8 21.2 19 19.7
%GEN 0.6 0.5 0.3 0.4 0.5
Treatment Animal 1 2 3 4 5
3-NP CN 855 880 848 872 847
PC 145 120 152 128 153
PMNC 12 22 11 13 13
%TOX 14.5 12 15.2 12.8 15.3
%GEN 1.2 2.2 1.1 1.3 1.3
Treatment Animal 1 2 3 4 5
CGA CN 803 801 810 784 797
PC 197 199 190 216 203
PMNC 5 4 6 6 5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 796 799 801 789 795
PC 204 201 199 211 205
PMNC 3 4 4 4 5
%TOX 20.4 20.1 19.9 21.1 20.5
%GEN 0.3 0.4 0.4 0.4 0.5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 825 834 796 820 853
PC 175 166 204 180 147
PMNC 7 5 6 7 10
%TOX 17.5 16.6 20.4 18 14.7
%GEN 0.7 0.5 0.6 0.7 1
%TOX 19.7 19.9 19 21.6 20.3
%GEN 0.5 0.4 0.6 0.6 0.5
Extraction and Characterization of CGA: Aerial parts of B. scordioides (300 g) were dried, ground and extracted with hexane and methanol in succession. The methanolic portion was evaporated under reduced pressure at 55 °C to obtain a syrup residue (30 g). CGA was isolated from methanolic extract by open column chromatography using SiO2 [2]. Micronuclei Assay: Samples of peripheral blood were obtained from the mice caudal vein at 24 h, 48 h, 72 h, 96 h, 120 h and 144 h after starting each treatment in each experimental group. A drop of blood was placed on a glass slide (3 slides per mice) and fixed with methanol for further H&E staining for 10 min.
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Table 5 Data obtained at 120 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 120 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
803 769 796 803 805
197 231 204 193 195
3 5 2 3 3
19.7 23.1 20.4 19.3 19.5
0.3 0.5 0.2 0.3 0.3
Treatment Animal 1 2 3 4 5
Buffer CN 805 816 766 808 789
PC 195 184 234 192 211
PMNC 3 4 5 3 4
%TOX 19.5 18.4 23.4 19.2 21.1
%GEN 0.3 0.4 0.5 0.3 0.4
Treatment Animal 1 2 3 4 5
3-NP CN 888 873 868 863 871
PC 112 127 132 137 129
PMNC 10 16 17 16 15
%TOX 11 12.7 13.2 13.7 12.9
%GEN 1 1.6 1.7 1.6 1.5
Treatment CGA Animal 1 2 3 4 5
CN 820 793 779 800 808
PC 180 207 221 200 192
PMNC 4 5 4 6 10
%TOX 18 20.7 22.1 20 19.2
%GEN 0.4 0.5 0.4 0.6 1
PMNC 17 14 15 16 15
%TOX 17.6 19.1 18.3 17.9 18.3
%GEN 1.7 1.4 1.5 1.6 1.5
Treatment Animal 1 2 3 4 5
3-NP þ CGA CN 824 809 817 821 817
PC 176 191 183 179 183
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 787 819 785 800 771
PC 213 181 215 200 229
PMNC 3 3 3 4 4
%TOX 21.3 18.1 21.5 20 22.9
%GEN 0.3 0.3 0.3 0.4 0.4
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 816 824 783 829 858
PC 184 176 217 171 142
PMNC 9 8 7 7 12
%TOX 18.4 17.6 21.7 17.1 14.2
%GEN 0.9 0.8 0.8 0.7 1.2
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Table 6 Data obtained at 144 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 144 h (6 days) Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
802 793 799 802 816
198 207 201 198 184
2 3 0 3 2
19.8 20.7 20.1 19.8 18.4
0.2 0.3 0 0.3 0.2
Treatment Animal 1 2 3 4 5
Buffer CN 806 809 773 819 807
PC 194 191 227 181 193
PMNC 2 4 5 4 3
%TOX 19.4 19.2 22.7 18.1 19.3
%GEN 0.2 0.4 0.5 0.4 0.3
Treatment Animal 1 2 3 4 5
3-NP CN 851 877 837 840 845
PC 149 123 163 160 155
PMNC 19 16 19 17 17
%TOX 14.9 12.3 16.3 16 15.5
%GEN 1.9 1.6 1.9 1.7 1.7
Treatment Animal 1 2 3 4 5
3-NP þ CGA CN 833 814 821 836 820
PC 167 186 179 165 180
PMNC 11 12 15 13 15
%TOX 6.7 18.6 17.9 16.5 18
%GEN 1.1 1.2 1.5 1.3 1.5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 770 795 767 766 753
PC 230 205 233 234 247
PMNC 2 3 3 3 4
%TOX 23 20.5 23.3 23.4 24.7
%GEN 0.3 0.3 0.3 0.3 0.4
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 830 817 813 825 827
PC 170 183 187 175 173
PMNC 5 6 12 12 7
%TOX 17 18.3 18.7 17.5 17.3
%GEN 0.5 0.6 1.2 1.2 0.7
Polychromatic and normochromatic cells of peripheral blood were counted with the aid of an optic microscope (Leica, Microsystems AG). Celle were classified in normochromatic (CN), polychromatic or (PC), polychromatic with micronuclei cells (MNC) in 1000 observed cells per each experimental condition in every evaluated time. Genotoxicity index (%) and toxicity index (%) was calculated through the Hayashi and cols., method [3].
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Table 7 Global Toxicity and Genotoxicity obtained by averaging data condition for every time evaluated are display in this table. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/ CGA). Global Toxicity Time
Negative Control
Ifosfamide
Buffer
3-NP
CGA
3-NP þ CGA
P/CGA, 3-NP þ CGA
P/CGA, 3-NP
24 h 48 h 72 h 96 h 120 h
19.84 20.04 20.42 19.82 20.4
15.74 13.82 19.42
20.24 20.12 20.28 20.06 20.32
10.36 13.92 14.12 13.96 12.7
17.96 19.68 19.62 20.1 20
17.2 16.72 16.36 17.86 18.24
19.72 19.84 20.66 20.4 20.76
14.86 15.66 14.96 17.44 17.8
Global Genotoxicity Time
Negative Control
Ifosfamide
Buffer
3-NP
CGA
3-NP þ CGA
P/CGA, 3-NP þ CGA
P/CGA, 3-NP
24 h 48 h 72 h 96 h 120 h
0.36 0.4 0.28 0.4 0.32
2.02 1.66 2.36
0.34 0.52 0.46 0.46 0.38
1.64 1.08 1.8 1.42 1.48
0.34 0.54 0.54 0.52 0.58
1.12 1.08 1.02 1.66 1.54
0.5 0.38 0.34 0.4 0.34
0.92 0.66 0.76 0.7 0.88
Funding sources This work was supported by CONACyT Grant no. 180660 to EHE.
Transparency document. Supporting information Transparency data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2017.07.004.
References [1] N. Alarcón-Herrera, S. Flores-Maya, B. Bellido, A.M. García-Bores, E. Mendoza, G. Ávila-Acevedo, E. Hernández-Echeagaray, Protective effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity, Food Chem. Toxicol. (2017), http://dx.doi.org/10.1016/j.fct.2017.04.048. [2] M.L. Suárez-Quiroz, A. Alonso-Campos, G. Valerio-Alfaro, O. González-Ríos, P. Villeneuve, M.C. Figueroa-Espinoza, Isolation of green coffee chlorogenic acids using activated carbon, J. Food Comp. Anal. 33 (2014) 55–58. [3] M. Hayashi, T. Morita, Y. Kodama, T. Sofuni, M. Ishidate Jr, The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides, Mutat. Res. 245 (1990) 245–249.
Contents lists available at ScienceDirect
Data in Brief journal homepage: www.elsevier.com/locate/dib
Data Article
Raw data of the effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity Alarcón-Herrera Norberto a, Flores-Maya Saúl a, Bellido Belén b, García-Bores Ana M.c, Mendoza Ernesto b, Ávila-Acevedo Guillermo c, Hernández-Echeagaray Elizabeth b,n a Laboratorio de Recursos Naturales, UBIPRO, FES-Iztacala, Universidad, Nacional Autónoma de México, México b Laboratorio de Neurofisiología del desarrollo y la neurodegeneración UBIMED, FES-Iztacala, Universidad, Nacional Autónoma de México, México c Laboratorio de Fitoquímica, UBIPRO, FES-Iztacala, Universidad, Nacional Autónoma de México, México
a r t i c l e i n f o
abstract
Article history: Received 9 May 2017 Received in revised form 12 June 2017 Accepted 5 July 2017 Available online 12 July 2017
The raw data showed in this article comes from the published research article entitled “Protective effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity” Food Chem Toxicol. 2017 May 3. pii: S0278-6915(17)30226-0. http://dx. doi.org/DOI:10.1016/j.fct.2017.04.048. [1]. Data illustrates antitoxic and antigenotoxic effects of Chlorogenic acid (CGA) on toxicity and genotoxicity produced by the in vivo treatment with mitochondria toxin 3-Nitropropionic acid (3-NP) in mice. Toxicity and genotoxicity was evaluated in erythrocytes of peripheral blood through the micronuclei assay. Data was share at the Elsevier repository under the reference number FCT9033. & 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: 3-NP CGA Toxicity Genotoxicity Data
n
DOI of original article: http://dx.doi.org/10.1016/j.fct.2017.04.048 Corresponding author. E-mail address: [email protected] (H.-E. Elizabeth).
http://dx.doi.org/10.1016/j.dib.2017.07.004 2352-3409/& 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
124
A.-H. Norberto et al. / Data in Brief 14 (2017) 123–131
Specifications Table Subject area More specific subject area Type of data How data was acquired
Data format Experimental factors
Experimental features Data source location Data accessibility
Biology Toxicology Tables Data from erythrocytes from peripheral blood, stained with H&E were classified in Normochromatic, Polychromatic or Polychromatic with micronuclei cells. 1000 cells per each condition were counted with the aid of by the aid of an optic microscope (Leica, Microsystems AG). Raw data In order to determine toxicity and genotoxicity of 3-NP, as well as protective effects of CGA, 6 experimental groups were evaluated; Negative control, Control (PB), 3-NP, CGA, 3-NP þ CA, P/CA, 3-NP þ CA and P/CA, 3-NP Each treatment lasted for 5 days except those where 5 days of pretreatment was present. To evaluate toxic and genotoxic effect of 3-NP and the antitoxic and antigenotoxic effects of CGA in erythrocytes. México City, México Data are available in this article and were place also in a public repository provided by Elsevier submission system, under reference number FCT9033
Value of the data
Data displays Normochromatic, Polychromatic or Polychromatic cells with micronuclei in the different experimental conditions and can be used by other research groups.
Toxicity and Genotoxicity were measured in peripheral blood by using the micronuclei assay. Τhese data are important because few studies have carried out to evaluate toxicity and genotoxicity of 3-NP which can be found in plants like sugar cane that are eaten by cattle and humans.
Data about CGA protective effects are important because CGA is found in a variety of food products which can help to protect the health of living organisms.
1. Data Raw data presented in this paper gives information about protective role of CGA on toxic and genotoxic effects of 3-NP in erythrocytes from peripheral blood. Data are shown in Tables 1–7 which illustrate the effect in each evaluated time.
2. Experimental design, materials and methods To evaluate toxic effect of 3-NP and the antitoxic effect of Chlorogenic acid, mice were randomly assigned to one of the following experimental groups: negative control, ifosfamide (positive control), buffer, 3-nitropropionic acid (3-NP), Chlorogenic acid (CGA), 3-NP þ Chlorogenic acid without pretreatment (3-NP þ CA, W/P), 3-NP þ Chlorogenic acid together with pretreatment of CA per 5 days (P/CA, 3-NP þ CA), pretreatment with CA for 5 days and later treatment of 3-NP alone, (P/CA, 3-NP). All groups were treated for 5 days with i.p. doses of 3-NP (15 mg/kg), CGA (100 mg/kg).
A.-H. Norberto et al. / Data in Brief 14 (2017) 123–131
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Table 1 Data obtained at 24 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 24 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
808 803 784 798 813
192 197 216 202 187
5 5 4 2 2
19.2 19.7 21.6 20.2 18.5
0.5 0.5 0.4 0.2 0.2
Treatment Animal 1 2 3 4 5
Ifosfamide CN 860 875 801 855 831
PC 149 125 199 145 169
PMNC 21 22 25 20 13
%TOX 14.9 12.5 19.9 14.5 16.9
%GEN 2.1 2.2 2.5 2 1.3
Treatment Animal 1 2 3 4 5
Buffer CN 811 787 771 814 805
PC 189 213 229 186 195
PMNC 3 5 2 4 3
%TOX 18.9 21.3 22.9 18.6 19.5
%GEN 0.3 0.5 0.2 0.4 0.3
Treatment Animal 1 2 3 4 5
3-NP CN 878 889 895 917 933
PC 122 111 105 83 97
PMNC 20 19 15 15 13
%TOX 12.2 11.1 10.5 8.3 9.7
%GEN 2 1.9 1.5 1.5 1.3
Treatment Animal 1 2 3 4 5
CGA CN 835 828 817 810 812
PC 165 172 183 190 188
PMNC 3 5 4 3 2
%TOX 16.5 17.2 18.3 19 18.8
%GEN 0.3 0.5 0.4 0.3 0.2
Treatment Animal 1 2 3 4 5
3NP þ CGA CN 843 840 835 810 812
PC 157 160 165 190 188
PMNC 10 10 13 11 12
%TOX 15.7 16.0 16.5 19.0 18.8
%GEN 1 1 1.3 1.1 1.2
Treatment
P/CGA, 3NP þ CGA CN 797 813 801 796 807 830 839 848 885 855
PMNC 6 8 3 3 5 11 9 10 8 8
%TOX 20.3 18.7 19.9 20.4 19.3 17 16.1 15.2 11.5 14.5
%GEN 0.6 0.8 0.3 0.3 0.5 1.1 0.9 1 0.8 0.8
Animal 1 2 3 4 5 1 2 3 4 5
PC 203 187 199 204 193 170 161 152 115 145
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Table 2 Data obtained at 48 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 48 h Treatment Animal
Negative control CN
PC
PMC
%TOX
%GEN
1 2 3 4 5
789 783 810 801 810
211 217 190 194 190
5 3 6 5 1
21.1 21.7 19 19.4 19
0.5 0.3 0.6 0.5 0.1
Treatment Animal 1 2 3 4 5
Ifosfamide CN 857 862 877 859 865
PC 143 148 123 141 135
PMNC 15 17 15 18 18
%TOX 14.3 14.8 12.3 14.2 13.5
%GEN 1.5 1.7 1.5 1.8 1.8
Treatment Animal 1 2 3 4 5
Buffer CN 817 807 760 820 790
PC 183 193 240 180 210
PMNC 5 6 5 5 5
%TOX 18.3 19.3 24 18 21
%GEN 0.5 0.6 0.5 0.5 0.5
Treatment Animal 1 2 3 4 5
3-NP CN 870 891 816 850 877
PC 130 109 184 150 123
PMNC 11 10 13 10 10
%TOX 13 10.9 18.4 15 12.3
%GEN 1.1 1 1.3 1 1
Treatment Animal 1 2 3 4 5 Treatment Animal 1 2 3 4 5
CGA CN 793 806 807 800 810 3-NP þ CGA CN 847 855 833 832 827
PC 207 194 193 200 190
PMNC 4 7 5 6 5
%TOX 20.7 19.4 19.3 20 19
%GEN 0.4 0.7 0.5 0.6 0.5
PC 153 165 167 168 183
PMNC 10 11 10 11 12
%TOX 15.3 16.5 16.7 16.8 18.3
%GEN 1 1.1 1 1.1 1.2
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 797 830 794 788 799
PC 203 170 206 212 201
PMNC 2 4 2 6 5
%TOX 20.3 17 20.6 21.2 20.1
%GEN 0.2 0.4 0.2 0.6 0.5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 857 818 870 876 820
PC 163 182 130 128 180
PMNC 7 8 10 2 6
%TOX 16.3 18.2 13 12.8 18
%GEN 0.7 0.8 1 0.2 0.6
A.-H. Norberto et al. / Data in Brief 14 (2017) 123–131
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Table 3 Data obtained at 72 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 72 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
794 799 799 791 795
205 201 201 209 205
3 3 3 2 3
20.5 20.1 20.1 20.9 20.5
0.3 0.3 0.3 0.2 0.3
Treatment Animal 1 2 3 4 5
Ifosfamide CN 832 838 751 885 783
PC 168 162 249 175 217
PMNC 25 26 25 24 17
%TOX 16.8 16.2 24.9 17.5 21.7
%GEN 2.5 2.6 2.5 2.5 1.7
Treatment Animal 1 2 3 4 5
Buffer CN 803 801 756 826 800
PC 197 199 244 174 200
PMNC 5 4 5 4 5
%TOX 19.7 19.9 24.4 17.4 20
%GEN 0.5 0.4 0.5 0.4 0.5
Treatment Animal 1 2 3 4 5
3-NP CN 879 862 860 838 855
PC 121 138 140 162 145
PMNC 20 17 17 19 17
%TOX 12.1 13.8 14 16.2 14.5
%GEN 2 1.7 1.7 1.9 1.7
Treatment Animal 1 2 3 4 5
CGA CN 799 827 785 808 800
PMNC 4 5 4 8 6
%TOX 20.1 17.3 21.5 19.2 20
%GEN 0.4 0.5 0.4 0.8 0.6
Treatment Animal 1 2 3 4 5
3-NP þ CGA CN 820 821 816 810 815
PMNC 9 9 12 11 10
%TOX 18 17.9 18.4 19 8.5
%GEN 0.9 0.9 1.2 1.1 1
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 790 795 796 789 797
PC 210 205 204 211 203
PMNC 5 3 2 3 4
%TOX 21 20.5 20.4 21.1 20.3
%GEN 0.5 0.3 0.2 0.3 0.4
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 846 832 868 857 849
PC 154 168 132 143 151
PMNC 7 7 10 5 9
%TOX 15.4 16.8 13.2 14.3 15.1
%GEN 0.7 0.7 1 0.5 0.9
PC 201 173 215 192 200 PC 180 179 184 190 185
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A.-H. Norberto et al. / Data in Brief 14 (2017) 123–131
Table 4 Data obtained at 96 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 96 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
787 813 817 793 799
213 187 183 207 201
5 3 1 6 5
21.3 18.7 18.3 20.7 20.1
0.5 0.3 0.1 0.6 0.5
Treatment Animal 1 2 3 4 5
Buffer CN 804 792 788 810 803
PC 196 208 212 190 197
PMNC 6 5 3 4 5
%TOX 19.6 20.8 21.2 19 19.7
%GEN 0.6 0.5 0.3 0.4 0.5
Treatment Animal 1 2 3 4 5
3-NP CN 855 880 848 872 847
PC 145 120 152 128 153
PMNC 12 22 11 13 13
%TOX 14.5 12 15.2 12.8 15.3
%GEN 1.2 2.2 1.1 1.3 1.3
Treatment Animal 1 2 3 4 5
CGA CN 803 801 810 784 797
PC 197 199 190 216 203
PMNC 5 4 6 6 5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 796 799 801 789 795
PC 204 201 199 211 205
PMNC 3 4 4 4 5
%TOX 20.4 20.1 19.9 21.1 20.5
%GEN 0.3 0.4 0.4 0.4 0.5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 825 834 796 820 853
PC 175 166 204 180 147
PMNC 7 5 6 7 10
%TOX 17.5 16.6 20.4 18 14.7
%GEN 0.7 0.5 0.6 0.7 1
%TOX 19.7 19.9 19 21.6 20.3
%GEN 0.5 0.4 0.6 0.6 0.5
Extraction and Characterization of CGA: Aerial parts of B. scordioides (300 g) were dried, ground and extracted with hexane and methanol in succession. The methanolic portion was evaporated under reduced pressure at 55 °C to obtain a syrup residue (30 g). CGA was isolated from methanolic extract by open column chromatography using SiO2 [2]. Micronuclei Assay: Samples of peripheral blood were obtained from the mice caudal vein at 24 h, 48 h, 72 h, 96 h, 120 h and 144 h after starting each treatment in each experimental group. A drop of blood was placed on a glass slide (3 slides per mice) and fixed with methanol for further H&E staining for 10 min.
A.-H. Norberto et al. / Data in Brief 14 (2017) 123–131
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Table 5 Data obtained at 120 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 120 h Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
803 769 796 803 805
197 231 204 193 195
3 5 2 3 3
19.7 23.1 20.4 19.3 19.5
0.3 0.5 0.2 0.3 0.3
Treatment Animal 1 2 3 4 5
Buffer CN 805 816 766 808 789
PC 195 184 234 192 211
PMNC 3 4 5 3 4
%TOX 19.5 18.4 23.4 19.2 21.1
%GEN 0.3 0.4 0.5 0.3 0.4
Treatment Animal 1 2 3 4 5
3-NP CN 888 873 868 863 871
PC 112 127 132 137 129
PMNC 10 16 17 16 15
%TOX 11 12.7 13.2 13.7 12.9
%GEN 1 1.6 1.7 1.6 1.5
Treatment CGA Animal 1 2 3 4 5
CN 820 793 779 800 808
PC 180 207 221 200 192
PMNC 4 5 4 6 10
%TOX 18 20.7 22.1 20 19.2
%GEN 0.4 0.5 0.4 0.6 1
PMNC 17 14 15 16 15
%TOX 17.6 19.1 18.3 17.9 18.3
%GEN 1.7 1.4 1.5 1.6 1.5
Treatment Animal 1 2 3 4 5
3-NP þ CGA CN 824 809 817 821 817
PC 176 191 183 179 183
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 787 819 785 800 771
PC 213 181 215 200 229
PMNC 3 3 3 4 4
%TOX 21.3 18.1 21.5 20 22.9
%GEN 0.3 0.3 0.3 0.4 0.4
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 816 824 783 829 858
PC 184 176 217 171 142
PMNC 9 8 7 7 12
%TOX 18.4 17.6 21.7 17.1 14.2
%GEN 0.9 0.8 0.8 0.7 1.2
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Table 6 Data obtained at 144 h measurements. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/CGA). 144 h (6 days) Treatment Animal
Negative Control CN
PC
PMNC
%TOX
%GEN
1 2 3 4 5
802 793 799 802 816
198 207 201 198 184
2 3 0 3 2
19.8 20.7 20.1 19.8 18.4
0.2 0.3 0 0.3 0.2
Treatment Animal 1 2 3 4 5
Buffer CN 806 809 773 819 807
PC 194 191 227 181 193
PMNC 2 4 5 4 3
%TOX 19.4 19.2 22.7 18.1 19.3
%GEN 0.2 0.4 0.5 0.4 0.3
Treatment Animal 1 2 3 4 5
3-NP CN 851 877 837 840 845
PC 149 123 163 160 155
PMNC 19 16 19 17 17
%TOX 14.9 12.3 16.3 16 15.5
%GEN 1.9 1.6 1.9 1.7 1.7
Treatment Animal 1 2 3 4 5
3-NP þ CGA CN 833 814 821 836 820
PC 167 186 179 165 180
PMNC 11 12 15 13 15
%TOX 6.7 18.6 17.9 16.5 18
%GEN 1.1 1.2 1.5 1.3 1.5
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP þ CGA CN 770 795 767 766 753
PC 230 205 233 234 247
PMNC 2 3 3 3 4
%TOX 23 20.5 23.3 23.4 24.7
%GEN 0.3 0.3 0.3 0.3 0.4
Treatment Animal 1 2 3 4 5
P/CGA, 3-NP CN 830 817 813 825 827
PC 170 183 187 175 173
PMNC 5 6 12 12 7
%TOX 17 18.3 18.7 17.5 17.3
%GEN 0.5 0.6 1.2 1.2 0.7
Polychromatic and normochromatic cells of peripheral blood were counted with the aid of an optic microscope (Leica, Microsystems AG). Celle were classified in normochromatic (CN), polychromatic or (PC), polychromatic with micronuclei cells (MNC) in 1000 observed cells per each experimental condition in every evaluated time. Genotoxicity index (%) and toxicity index (%) was calculated through the Hayashi and cols., method [3].
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Table 7 Global Toxicity and Genotoxicity obtained by averaging data condition for every time evaluated are display in this table. Normochromatic erythrocyte cells (CN), Polychromatic erythrocyte cells (PC), Polychromatic cells with micronuclei (PMNC), Toxicity (TOX), Genotoxicity (Gen), 3-Nitropropionic acid (3-NP), Chlorogenic acid (CGA), Pretreatment of Chlorogenic acid (P/ CGA). Global Toxicity Time
Negative Control
Ifosfamide
Buffer
3-NP
CGA
3-NP þ CGA
P/CGA, 3-NP þ CGA
P/CGA, 3-NP
24 h 48 h 72 h 96 h 120 h
19.84 20.04 20.42 19.82 20.4
15.74 13.82 19.42
20.24 20.12 20.28 20.06 20.32
10.36 13.92 14.12 13.96 12.7
17.96 19.68 19.62 20.1 20
17.2 16.72 16.36 17.86 18.24
19.72 19.84 20.66 20.4 20.76
14.86 15.66 14.96 17.44 17.8
Global Genotoxicity Time
Negative Control
Ifosfamide
Buffer
3-NP
CGA
3-NP þ CGA
P/CGA, 3-NP þ CGA
P/CGA, 3-NP
24 h 48 h 72 h 96 h 120 h
0.36 0.4 0.28 0.4 0.32
2.02 1.66 2.36
0.34 0.52 0.46 0.46 0.38
1.64 1.08 1.8 1.42 1.48
0.34 0.54 0.54 0.52 0.58
1.12 1.08 1.02 1.66 1.54
0.5 0.38 0.34 0.4 0.34
0.92 0.66 0.76 0.7 0.88
Funding sources This work was supported by CONACyT Grant no. 180660 to EHE.
Transparency document. Supporting information Transparency data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2017.07.004.
References [1] N. Alarcón-Herrera, S. Flores-Maya, B. Bellido, A.M. García-Bores, E. Mendoza, G. Ávila-Acevedo, E. Hernández-Echeagaray, Protective effects of Chlorogenic acid in 3-Nitropropionic acid induced toxicity and genotoxicity, Food Chem. Toxicol. (2017), http://dx.doi.org/10.1016/j.fct.2017.04.048. [2] M.L. Suárez-Quiroz, A. Alonso-Campos, G. Valerio-Alfaro, O. González-Ríos, P. Villeneuve, M.C. Figueroa-Espinoza, Isolation of green coffee chlorogenic acids using activated carbon, J. Food Comp. Anal. 33 (2014) 55–58. [3] M. Hayashi, T. Morita, Y. Kodama, T. Sofuni, M. Ishidate Jr, The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides, Mutat. Res. 245 (1990) 245–249.
