0021-972X/91/7206-1328$03.00/0 Journal of Clinical Endocrinology and Metabolism Copyright © 1991 by The Endocrine Society
Vol. 72, No. 6 Printed in U.S.A.
Recombinant Human Thyroid-Stimulating Hormone: Initial Bioactivity Assessment using Human Fetal Thyroid Cells* GEROLD K. HUBERf, PO FONG, ERLINDA S. CONCEPCION, AND TERRY F. DAVIES Division of Endocrinology, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029
0.55, similar to that of purified pituitary hTSH standards. Furthermore, rec-hTSH induced thyroid epithelial cell growth, as evidenced by a decrease in thyroid cell doubling time from 54 ± 2.1 to 31 ± 1.7 h (P < 0.005). Hence, rec-hTSH is a potent glycoprotein hormone preparation when measured in a homologous human thyroid cell culture system. Rec-hTSH could serve as a future definitive International Standard and has the potential for a useful diagnostic and therapeutic reagent. (
Vol. 72, No. 6 Printed in U.S.A.
Recombinant Human Thyroid-Stimulating Hormone: Initial Bioactivity Assessment using Human Fetal Thyroid Cells* GEROLD K. HUBERf, PO FONG, ERLINDA S. CONCEPCION, AND TERRY F. DAVIES Division of Endocrinology, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029
0.55, similar to that of purified pituitary hTSH standards. Furthermore, rec-hTSH induced thyroid epithelial cell growth, as evidenced by a decrease in thyroid cell doubling time from 54 ± 2.1 to 31 ± 1.7 h (P < 0.005). Hence, rec-hTSH is a potent glycoprotein hormone preparation when measured in a homologous human thyroid cell culture system. Rec-hTSH could serve as a future definitive International Standard and has the potential for a useful diagnostic and therapeutic reagent. (
