THROMBOSIS RESEARCH 67; 655-663, 1992 0049-3848/92 $5.00 + .OO Printed in the USA. Copyright (c) 1992 Pergamon Press Ltd. All rights reserved.
REDIJCED t.‘l~NC”l‘IONS OF INTRACELLULAR Ca?+ IN AGGREGATION, SECRETION AND PROTEIN PHOSPHORYLATION OF PERMEABILIZED PLATELETS FROhl STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS
hl. Il\cth 11. T. Onda 2’. I. Tomita 2) and T. Tomita 1) l ) Ciradu~~k School 01‘Hcalth Scicnces. 2) School of Pharmaceutical Sciences, Univcrsity Shiluoka,
F3- I . Yada. Shi/uoka
ot
4137. Japan.
(Received 12.5.1992; accepted in revised form 17.7.1992 by Editor A. Takada)
ABSTRACT mcl 37kDu protein (P47) phosphorylation by various Aggrcgation. \ccretion agoniuts \uch as thrombin. ADP and ionophore A33 187 were markedly reduced in platelcts from strokc-prone spontaneously hypertensive rats (SHRSP) compared with thosc ocagc-matched Wistar Kyoto rat (WKY) platelets, suggesting defeetive functions of intraccllular Ca?+ in SHRSP platelets (Tomita et al. Hypertension 19x9: I-l: iO3-315). To clarify tbe mechanism of the platelet hypofunctilons, saponin pcrmeabili/.cd platelets were prepared to compare the responses of platelct\ (‘ram both rats in varying concentrations of extracellular Ca?+. The Icakagc of laclate dehydrogenase from saponin (IS pg/ml)-treated platelets was approx. 5 !; of total activity: the dcgree of the leakage in both platelets did nol diffcr. In sqonin-trcated plstelets, extracellular Ca?+ alone did not induce either or sccretion in both strains. Howevcr, in the presence of 1-oleoyl-zagg7 ~.x
REDIJCED t.‘l~NC”l‘IONS OF INTRACELLULAR Ca?+ IN AGGREGATION, SECRETION AND PROTEIN PHOSPHORYLATION OF PERMEABILIZED PLATELETS FROhl STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS
hl. Il\cth 11. T. Onda 2’. I. Tomita 2) and T. Tomita 1) l ) Ciradu~~k School 01‘Hcalth Scicnces. 2) School of Pharmaceutical Sciences, Univcrsity Shiluoka,
F3- I . Yada. Shi/uoka
ot
4137. Japan.
(Received 12.5.1992; accepted in revised form 17.7.1992 by Editor A. Takada)
ABSTRACT mcl 37kDu protein (P47) phosphorylation by various Aggrcgation. \ccretion agoniuts \uch as thrombin. ADP and ionophore A33 187 were markedly reduced in platelcts from strokc-prone spontaneously hypertensive rats (SHRSP) compared with thosc ocagc-matched Wistar Kyoto rat (WKY) platelets, suggesting defeetive functions of intraccllular Ca?+ in SHRSP platelets (Tomita et al. Hypertension 19x9: I-l: iO3-315). To clarify tbe mechanism of the platelet hypofunctilons, saponin pcrmeabili/.cd platelets were prepared to compare the responses of platelct\ (‘ram both rats in varying concentrations of extracellular Ca?+. The Icakagc of laclate dehydrogenase from saponin (IS pg/ml)-treated platelets was approx. 5 !; of total activity: the dcgree of the leakage in both platelets did nol diffcr. In sqonin-trcated plstelets, extracellular Ca?+ alone did not induce either or sccretion in both strains. Howevcr, in the presence of 1-oleoyl-zagg7 ~.x
