Accepted Manuscript Regular activity breaks combined with physical activity improves postprandial plasma triglyceride, non-esterified fatty acid and insulin responses in healthy, normal weight adults: a randomized crossover trial Ashleigh R. Homer, MSc, Stephen P. Fenemor, MPhEd, Tracy L. Perry, PhD, Nancy J. Rehrer, PhD, Claire M. Cameron, PhD, C Murray Skeaff, PhD, Meredith C. Peddie, PhD PII:

S1933-2874(17)30351-3

DOI:

10.1016/j.jacl.2017.06.007

Reference:

JACL 1137

To appear in:

Journal of Clinical Lipidology

Received Date: 8 March 2017 Revised Date:

7 June 2017

Accepted Date: 7 June 2017

Please cite this article as: Homer AR, Fenemor SP, Perry TL, Rehrer NJ, Cameron CM, Skeaff CM, Peddie MC, Regular activity breaks combined with physical activity improves postprandial plasma triglyceride, non-esterified fatty acid and insulin responses in healthy, normal weight adults: a randomized crossover trial, Journal of Clinical Lipidology (2017), doi: 10.1016/j.jacl.2017.06.007. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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ACCEPTED MANUSCRIPT

ACCEPTED MANUSCRIPT Title: Regular activity breaks combined with physical activity improves postprandial plasma triglyceride, non-esterified fatty acid and insulin responses in healthy, normal weight adults: a randomized crossover trial Short title: Breaking prolonged sitting and postprandial metabolism

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Authors: Ashleigh R Homer MSc, Stephen P Fenemor MPhEd, Tracy L Perry PhD, Nancy J Rehrer PhD, Claire M Cameron PhD, C Murray Skeaff PhD, Meredith C Peddie PhD

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Author Affiliations: Department of Human Nutrition, University of Otago (ARH, TLP, CMS, MCP) School of Physical Education, Sport and Exercise Sciences (SPF, NJR) Department of Preventive and Social Medicine (CMC)

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Corresponding Author: Meredith C Peddie, Department of Human Nutrition, University of Otago, PO Box 56, Dunedin, New Zealand. Phone: +64 3 479 8157, email: [email protected] Sources of Support: This research was generously supported by grants from: The National Heart Foundation of New Zealand, Lotteries Health Research, University of Otago. MCP is supported by a fellowship from The National Heart Foundation of New Zealand.

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Conflict of Interest: None

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ACCEPTED MANUSCRIPT Background: Compared to prolonged sitting, regular activity breaks immediately lower postprandial glucose and insulin, but not triglyceride responses. Postprandial triglycerides can be lowered by physical activity but the effect is often delayed by ~12-24 h

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Objective: To determine whether regular activity breaks affect postprandial triglyceride response in a delayed manner similar to physical activity.

Methods: In a randomized crossover trial, 36 adults (BMI 23.9 kg·m2 (SD

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3.9)) completed four two-day interventions: 1. Prolonged Sitting (SIT); 2.

Prolonged Sitting with 30 min of continuous walking (60% VO2max), at the end

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of Day 1 (SIT+PAD1); 3. Sitting with 2 min of walking (60% VO2max) every 30 min (RAB); 4. A combination of the continuous walking and regular activity breaks in 2 and 3 above (RAB+PAD1). Postprandial plasma triglyceride, nonesterified fatty acids (NEFA), glucose, and insulin responses were measured

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in venous blood over 5 h on Day 2.

Results: Compared to SIT, both RAB (difference: -43.61 mg·dL-1·5h; 95% CI –83.66 to -2.67; p=0.035) and RAB+PAD1 (-65.86 mg·dL-1·5h; 95% CI -112.14

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to -19.58; p=0.005) attenuated triglyceride tAUC. RAB+PAD1 produced the greatest reductions in insulin tAUC (-23%; 95% CI -12 to -31%; p