Vol.
170,
July
31, 1990
No.
2, 1990
BIOCHEMICAL
BIOPHYSICAL
RESEARCH
COMMUNICATIONS Pages
REGULATlON ALKALINIZATION
MARK
AND
OF cGMP
PRODUCTION
IN CULTURED RAT INNER DUCT CELLS
S. SISKIND,
EDWARD
A. ALEXANDER,
BY INTRACELLULAR MEDULLARY
AND
JOHN
COLLECTING
H. SCHWARTZ
THORNDIKE MEMORIAL LABORATORY, RENAL SECTION, BOSTON HOSPITAL, 818 HARRISON AVE., BOSTON, MA 02118 Received
June
15,
8604366
CITY
1990
We evaluated the relationship between cell pH and cGMP production in cultured rat renal inner medullary collecting duct cells. The cGMP level, 21%, was not different in control vs. alkalinized cells, 4e17 fmol/mg protein (~~0.5). 10-f fM atrial natriureitic peptide (ANF) enhanced cGMP production in alkalinized cells, 42634 vs. 141+9*. Conversely, alkalinization inhibited 10’4M nitroprusside (SNP) induced cGMP formation, 29+9 vs. 332?67*. Phosphodiesterase inhibition abolished the difference in cGMP production by ANF but did not reverse the inhibitory effect of alkalinization on SNP induced cGMP production. In rat renal inner medullary collecting duct cells, cellular alkalinization plays a significant role in the regulation of guanylate cyclase 01990 Academic Press, Inc. mediated cGMP production. l =p
170,
July
31, 1990
No.
2, 1990
BIOCHEMICAL
BIOPHYSICAL
RESEARCH
COMMUNICATIONS Pages
REGULATlON ALKALINIZATION
MARK
AND
OF cGMP
PRODUCTION
IN CULTURED RAT INNER DUCT CELLS
S. SISKIND,
EDWARD
A. ALEXANDER,
BY INTRACELLULAR MEDULLARY
AND
JOHN
COLLECTING
H. SCHWARTZ
THORNDIKE MEMORIAL LABORATORY, RENAL SECTION, BOSTON HOSPITAL, 818 HARRISON AVE., BOSTON, MA 02118 Received
June
15,
8604366
CITY
1990
We evaluated the relationship between cell pH and cGMP production in cultured rat renal inner medullary collecting duct cells. The cGMP level, 21%, was not different in control vs. alkalinized cells, 4e17 fmol/mg protein (~~0.5). 10-f fM atrial natriureitic peptide (ANF) enhanced cGMP production in alkalinized cells, 42634 vs. 141+9*. Conversely, alkalinization inhibited 10’4M nitroprusside (SNP) induced cGMP formation, 29+9 vs. 332?67*. Phosphodiesterase inhibition abolished the difference in cGMP production by ANF but did not reverse the inhibitory effect of alkalinization on SNP induced cGMP production. In rat renal inner medullary collecting duct cells, cellular alkalinization plays a significant role in the regulation of guanylate cyclase 01990 Academic Press, Inc. mediated cGMP production. l =p
