RESEARCH ARTICLE
Relationship between Heat-Labile Enterotoxin Secretion Capacity and Virulence in Wild Type Porcine-Origin Enterotoxigenic Escherichia coli Strains Prageeth Wijemanne1, Jun Xing1,2, Emil M. Berberov1,3, David B. Marx4, David H. Francis5, Rodney A. Moxley1*
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1 School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America, 2 Canada Immunoassay Development, Windsor, Ontario, Canada, 3 Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, 4 Department of Statistics, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America, 5 Department of Veterinary Science, South Dakota State University, Brookings, South Dakota, United States of America * [email protected]
OPEN ACCESS Citation: Wijemanne P, Xing J, Berberov EM, Marx DB, Francis DH, Moxley RA (2015) Relationship between Heat-Labile Enterotoxin Secretion Capacity and Virulence in Wild Type Porcine-Origin Enterotoxigenic Escherichia coli Strains. PLoS ONE 10(3): e0117663. doi:10.1371/journal.pone.0117663 Academic Editor: Gireesh Rajashekara, The Ohio State University, UNITED STATES Received: August 13, 2014 Accepted: December 30, 2014 Published: March 13, 2015 Copyright: © 2015 Wijemanne et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: Support was provided by the United States Department of Agriculture Multi-State Research Project NC1202, NEB-39-167. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Abstract Heat-labile enterotoxin (LT) is an important virulence factor secreted by some strains of enterotoxigenic Escherichia coli (ETEC). The prototypic human-origin strain H10407 secretes LT via a type II secretion system (T2SS). We sought to determine the relationship between the capacity to secrete LT and virulence in porcine-origin wild type (WT) ETEC strains. Sixteen WT ETEC strains isolated from cases of severe diarrheal disease were analyzed by GM1ganglioside enzyme-linked immunosorbent assay to measure LT concentrations in culture supernatants. All strains had detectable LT in supernatants by 2 h of culture and 1 strain, which was particularly virulent in gnotobiotic piglets (3030-2), had the highest LT secretion level all porcine-origin WT strains tested (P
Relationship between Heat-Labile Enterotoxin Secretion Capacity and Virulence in Wild Type Porcine-Origin Enterotoxigenic Escherichia coli Strains Prageeth Wijemanne1, Jun Xing1,2, Emil M. Berberov1,3, David B. Marx4, David H. Francis5, Rodney A. Moxley1*
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1 School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America, 2 Canada Immunoassay Development, Windsor, Ontario, Canada, 3 Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, 4 Department of Statistics, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America, 5 Department of Veterinary Science, South Dakota State University, Brookings, South Dakota, United States of America * [email protected]
OPEN ACCESS Citation: Wijemanne P, Xing J, Berberov EM, Marx DB, Francis DH, Moxley RA (2015) Relationship between Heat-Labile Enterotoxin Secretion Capacity and Virulence in Wild Type Porcine-Origin Enterotoxigenic Escherichia coli Strains. PLoS ONE 10(3): e0117663. doi:10.1371/journal.pone.0117663 Academic Editor: Gireesh Rajashekara, The Ohio State University, UNITED STATES Received: August 13, 2014 Accepted: December 30, 2014 Published: March 13, 2015 Copyright: © 2015 Wijemanne et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: Support was provided by the United States Department of Agriculture Multi-State Research Project NC1202, NEB-39-167. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Abstract Heat-labile enterotoxin (LT) is an important virulence factor secreted by some strains of enterotoxigenic Escherichia coli (ETEC). The prototypic human-origin strain H10407 secretes LT via a type II secretion system (T2SS). We sought to determine the relationship between the capacity to secrete LT and virulence in porcine-origin wild type (WT) ETEC strains. Sixteen WT ETEC strains isolated from cases of severe diarrheal disease were analyzed by GM1ganglioside enzyme-linked immunosorbent assay to measure LT concentrations in culture supernatants. All strains had detectable LT in supernatants by 2 h of culture and 1 strain, which was particularly virulent in gnotobiotic piglets (3030-2), had the highest LT secretion level all porcine-origin WT strains tested (P
