0013-7227/91/1285-2660$03.00/0 Endocrinology Copyright © 1991 by The Endocrine Society
Vol. 128, No. 5 Printed in U.S.A.
RENAL IGF-1 mRNA LEVELS ARE ENHANCED FOLLOWING UNILATERAL NEPHRECTOMY IN IMMATURE BUT NOT ADULT RATS Susan E. Mulroney*+, Aviad Haramati*, Charles T. Roberts, Jr.*, and Derek LeRoith* Department of Physiology and Biophysics, Georgetown University School of Medicine, Washington, DC*, and Laboratory of Cellular and Molecular Physiology, Diabetes Branch, NIDDK, NIH, Bethesda, MD* ABSTRACT: The increase in IGF-1 gene expression following unilateral nephrectomy (UNX) in adult rats is controversial. In this study we have examined whether developmental differences exist in the effect of UNX on IGF1 gene expression. Immature (23 days) and adult (4 months) Wistar rats underwent a sham operation or left UNX, and were sacrificed 24 or 48 hrs later. IGF-1 mRNA levels were determined in left (control) and right (compensated) kidneys using solution hybridization/RNase protection assays. By 48 hrs post-UNX, remnant kidneys had grown -20±l% in adult rats (P
Vol. 128, No. 5 Printed in U.S.A.
RENAL IGF-1 mRNA LEVELS ARE ENHANCED FOLLOWING UNILATERAL NEPHRECTOMY IN IMMATURE BUT NOT ADULT RATS Susan E. Mulroney*+, Aviad Haramati*, Charles T. Roberts, Jr.*, and Derek LeRoith* Department of Physiology and Biophysics, Georgetown University School of Medicine, Washington, DC*, and Laboratory of Cellular and Molecular Physiology, Diabetes Branch, NIDDK, NIH, Bethesda, MD* ABSTRACT: The increase in IGF-1 gene expression following unilateral nephrectomy (UNX) in adult rats is controversial. In this study we have examined whether developmental differences exist in the effect of UNX on IGF1 gene expression. Immature (23 days) and adult (4 months) Wistar rats underwent a sham operation or left UNX, and were sacrificed 24 or 48 hrs later. IGF-1 mRNA levels were determined in left (control) and right (compensated) kidneys using solution hybridization/RNase protection assays. By 48 hrs post-UNX, remnant kidneys had grown -20±l% in adult rats (P
