Proc. Natl. Acad. Sci. USA Vol. 87, pp. 5026-5030, July 1990 Cell Biology
Interleukin 1 pretreatment decreases ischemia/reperfusion injury (02 radicals/neutrophils/antioxidants/vascular injury/heart)
JAMES M. BROWN*, CARL W. WHITEt, LANCE S. TERADAtt, MICHAEL A. GROSSO*, PAUL F. SHANLEYt, DAVID W. MULVIN*, ANIRBAN BANERJEE*, GLENN J. R. WHITMAN*, ALDEN H. HARKEN*, AND JOHN E. REPINEtt§ Departments of *Surgery and *Medicine and the tWebb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, CO 80262
Communicated by David W. Talmage, April 12, 1990
diets containing 0.7 g of sodium tungstate (ICN) per kg of body weight or standard diets (2). In some experiments, sodium molybdate (60 mg/kg in 5.0 mM potassium phosphate buffer, pH 7.4) was given i.p. 6 hr before heart isolation. Rats were injected i.p. with 30 ug of human recombinant IL-la per kg of body weight (specific activity, 3 x 108 units/mg by D10 assay with
Interleukin 1 pretreatment decreases ischemia/reperfusion injury (02 radicals/neutrophils/antioxidants/vascular injury/heart)
JAMES M. BROWN*, CARL W. WHITEt, LANCE S. TERADAtt, MICHAEL A. GROSSO*, PAUL F. SHANLEYt, DAVID W. MULVIN*, ANIRBAN BANERJEE*, GLENN J. R. WHITMAN*, ALDEN H. HARKEN*, AND JOHN E. REPINEtt§ Departments of *Surgery and *Medicine and the tWebb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, CO 80262
Communicated by David W. Talmage, April 12, 1990
diets containing 0.7 g of sodium tungstate (ICN) per kg of body weight or standard diets (2). In some experiments, sodium molybdate (60 mg/kg in 5.0 mM potassium phosphate buffer, pH 7.4) was given i.p. 6 hr before heart isolation. Rats were injected i.p. with 30 ug of human recombinant IL-la per kg of body weight (specific activity, 3 x 108 units/mg by D10 assay with
