IJSEM Papers in Press. Published November 5, 2014 as doi:10.1099/ijs.0.064428-0
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Rhizobium yantingense sp. nov., a mineral-weathering
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bacterium
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Wei Chen, Xia-Fang Sheng, Lin-Yan He, and Zhi Huang
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Running title: Rhizobium yantingense sp. nov.
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Subject category: New taxa in Proteobacteria
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Author for correspondence:
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Xia-Fang Sheng
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[email protected]
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Key Laboratory of Agricultural Environment Microbiology, Ministry of Agriculture, College of
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Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain H66T is
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KC934840. The GenBank/EMBL/DDBJ accession number for the recA, atpD and glnII sequences
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of strain H66T are KM029983, KM029981 and KM029982, respectively.
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A Gram negative, rod-shaped bacterial strain, H66T, was isolated from the surfaces of
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weathered rock (purple siltstone) which was found in Yanting, Sichuan Province of China.
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Cells of strain H66T were motile with peritrichous flagella. Phylogenetic analysis based on
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16S rRNA gene sequences indicated that strain H66T belongs to the genus Rhizobium. It
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closely relates to Rhizobium huautlense SO2T (98.1 %), Rhizobium alkalisoli CCBAU 01393T
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(98.0 %), and Rhizobium cellulosilyticum ALA10B2T (98.0 %). Analysis of housekeeping
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genes recA, glnII and atpD showed low levels of sequence similarity (
1
Rhizobium yantingense sp. nov., a mineral-weathering
2
bacterium
3
Wei Chen, Xia-Fang Sheng, Lin-Yan He, and Zhi Huang
4 5 6 7 8
Running title: Rhizobium yantingense sp. nov.
9
Subject category: New taxa in Proteobacteria
10
Author for correspondence:
11
Xia-Fang Sheng
12
[email protected]
13 14 15 16 17 18 19 20 21 22
Key Laboratory of Agricultural Environment Microbiology, Ministry of Agriculture, College of
23
Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China
24 25
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain H66T is
26
KC934840. The GenBank/EMBL/DDBJ accession number for the recA, atpD and glnII sequences
27
of strain H66T are KM029983, KM029981 and KM029982, respectively.
28 29 1
30 31
A Gram negative, rod-shaped bacterial strain, H66T, was isolated from the surfaces of
32
weathered rock (purple siltstone) which was found in Yanting, Sichuan Province of China.
33
Cells of strain H66T were motile with peritrichous flagella. Phylogenetic analysis based on
34
16S rRNA gene sequences indicated that strain H66T belongs to the genus Rhizobium. It
35
closely relates to Rhizobium huautlense SO2T (98.1 %), Rhizobium alkalisoli CCBAU 01393T
36
(98.0 %), and Rhizobium cellulosilyticum ALA10B2T (98.0 %). Analysis of housekeeping
37
genes recA, glnII and atpD showed low levels of sequence similarity (
